Dasatinib (DAS), apigenin (APG), and ibrutinib (IBR), were obtained from Sigma-Aldrich (St. Louis, MI, USA). Acetonitrile, formic acid, methanol, and ammonium acetate were purchased from BDH, Pool (UK) and antibodies anti-P-gp/MDR1/ABCB1, anti-BCRP/ABCG2, Anti-CYP3 A2, and anti-β-actin antibodies were obtained from Santacruz (USA).
Ammonium acetate
Manufactured by BD
Sourced in United Kingdom
Ammonium acetate is a chemical compound with the formula CH3COO-NH4+. It is a crystalline, colorless salt that is highly soluble in water. Ammonium acetate serves as a buffer solution and is commonly used in analytical laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by BD (1 mentions)
Ibrutinib, by Merck Group (1 mentions)
Acetonitrile, by BD (1 mentions)
Methanol, by BD (1 mentions)
Anti bcrp abcg2, by Santa Cruz Biotechnology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Glacial acetic acid, by BD (1 mentions)
High performance liquid chromatography (hplc), by Merck Group (1 mentions)
Homocysteine, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by BD (1 mentions)
Hydrochloric acid (hcl), by BD (1 mentions)
Sodium hydroxide (naoh), by BD (1 mentions)
2 9 dimethyl 1 10 phenanthroline neocuproine, by Merck Group (1 mentions)
Tert butyl hydroperoxide, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Sodium azide, by BD (1 mentions)
3 protocols using ammonium acetate
1
Quantitative Analysis of Drug Transporters
2
FST Assay and Neurotransmitter Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twenty eight groups of mice (n=8) were used and three sets of experiments were performed. In the first set, mice were subjected to FST and then 1h later, the serum and brain levels of F and NF were measured by liquid chromatography/mass spectrometry (LC/MS). Propranolol hydrochloride was used as an internal standard (IS) and ultrapure water (Purelab Classic water purification system, Elga, UK) was used. All solvents were of HPLC grade (Merck, Darmstadt, Germany). Ammonium acetate and glacial acetic acid were of analytical grade (BDH, Poole, England). In the second set, the influence of pretreatment with PCPA on the effects of drugs in the FST was tested. In the third set, effect of drugs (at the same doses used in the FST) on locomotor activity of mice was measured using the open field test.
3
Redox Regulation Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ammonium acetate, calcium chloride, copper(II) chloride, dipotassium phosphate (K2HPO4), hydrochloric acid, sodium dihydrogen phosphate dihydrate (NaH2PO4. 2H2O), hydrogen peroxide (30%), di-sodium hydrogen phosphate dihydrate (Na2HPO4.2H2O), sodium azide, sodium hydroxide, sodium nitrate, and trichloroactetic acid were purchased from BDH.
Bovine serum albumin, glutathione, homocysteine, neocuproine (2,9-dimethyl-1,10phenanthroline) and tert-butyl hydroperoxide were purchased from Sigma-Aldrich.
Bovine serum albumin, glutathione, homocysteine, neocuproine (2,9-dimethyl-1,10phenanthroline) and tert-butyl hydroperoxide were purchased from Sigma-Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!