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Hrp linked mouse igg

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HRP-linked mouse IgG is a laboratory reagent used to detect and quantify the presence of target proteins in biological samples. It consists of mouse immunoglobulin G (IgG) molecules covalently linked to the enzyme horseradish peroxidase (HRP). This reagent can be used in various immunoassay techniques, such as Western blotting and ELISA, to amplify and visualize the signal generated by the binding of the IgG to its target antigen.

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Lab products found in correlation

Hrp conjugated anti β actin, by Merck Group (2 mentions) Azd1208, by Adooq (2 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti pim2, by Cell Signaling Technology (1 mentions) Anti pim3, by Cell Signaling Technology (1 mentions) Anti pim1, by Cell Signaling Technology (1 mentions) Pim447, by Novartis (1 mentions) Anti irs 1, by Merck Group (1 mentions) Fitc conjugated anti human cd45, by BioLegend (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti sesn2, by Proteintech (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Anti phospho tsc2, by Cell Signaling Technology (1 mentions) Anti phospho gsk3β, by Cell Signaling Technology (1 mentions) Apc conjugated anti mouse cd45, by BioLegend (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions) Anti phospho 4ebp1, by Cell Signaling Technology (1 mentions) Anti ddit4, by Proteintech (1 mentions) Anti phospho nf kb p65, by Cell Signaling Technology (1 mentions)

4 protocols using hrp linked mouse igg

1

Probing Protein Kinase Signaling in Stem Cells

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Primary antibodies used for western blotting included anti‐PIM1 (Cell Signaling Technology, Cat # 2907), anti‐PIM2 (Cell Signaling Technology, Cat # 4730), anti‐PIM3 (Cell Signaling Technology, Cat # 4165), anti‐pIRS1‐S1101 (Cell Signaling Technology, Cat # S1101 Cat # 2385), anti‐insulin receptor substrate 1 (IRS1; Cell Signaling Technology, Cat # 06‐248), anti‐HA (Cell Signaling Technology, Cat # 14031), anti‐SOX2 [(E‐4) sc‐365823; Santa Cruz Biotech, Santa Cruz, CA, USA], anti‐OCT‐4 [(C‐10) sc‐5279; Santa Cruz Biotech], and anti‐NANOG [(5A10) sc‐134218; Santa Cruz Biotech]. HRP‐conjugated anti‐β‐actin (Cat # A3854) was purchased from Sigma‐Aldrich. HRP‐linked mouse IgG (Cat # NA931V) and rabbit IgG (Cat # NAV934V) were purchased from GE Healthcare Life Sciences (Princeton, NJ, USA).
Doxycycline (Dox) hydrochloride (Cat # D3447) was purchased from Sigma‐Aldrich. AZD1208 (Cat # A13203) was purchased from Adooq Bioscience (Irvine, CA, USA). Enza (MDV3100; Cat # S1250) was purchased from Selleckchem (Houston, TX, USA). PIM447 was a gift from Novartis (Basel, Switzerland).
Singh N., Padi S.K., Bearss J.J., Pandey R., Okumura K., Beltran H., Song J.H., Kraft A.S, & Olive V. (2020). PIM protein kinases regulate the level of the long noncoding RNA H19 to control stem cell gene transcription and modulate tumor growth. Molecular Oncology, 14(5), 974-990.
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2

Molecular Mechanisms Underlying mTOR Signaling Regulation

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The following antibodies were purchased from Cell Signaling Technology: anti-phospho- IRS1 (S1101 Cat # 2385), anti-phospho-S6 (S240/244, Cat # 5364), anti-phospho-TSC2 (S939 Cat # 3615), anti-TSC2 (Cat # 3990), anti-PTEN (Cat # 138G6), anti-Phospho-NF-kB p65 (S536 Cat # 93H1), anti-NF-kB p65 (Cat # D14E12), anti-phospho-4EBP1 (S65 Cat # 13443), anti-4EBP1(Cat # 9644), and anti-phospho-GSK3β (S9, Cat # 5558). Anti-IRS1 (Cat # 06–248) antibody was purchased from Merck Millipore. Anti-DDIT4 (Cat#10638–1-AP) and anti-SESN2 (Cat # 10795–1-AP) antibodies were purchased from ProteinTech Group. HRP conjugated anti-β-actin (Cat # A3854) was purchased from Sigma. HRP-linked mouse IgG (Cat # NA931V) and rabbit IgG (Cat # NAV934V) were purchased from GE Healthcare Life Sciences. IgG (Isotype control Cat # 400107), FITC conjugated anti-human CD45 (Cat # 304006), and APC conjugated anti-mouse CD45 (Cat # 103112) were purchased from BioLegend. Anti-S6 (Cat # SC74459) antibody was purchased from Santa Cruz laboratories. LGB-321 (Cat # A14420), AZD1208 (Cat # A13203), and IKK16 (# A12836) were purchased from Adooq bioscience. TNF-α (Cat # PHC3015) was purchased from Fisher scientific.
Supplementary Methods include a description of the Western Blot, RNA-Seq data analysis, GSEA analysis, RPPA, Active module analysis, Lentiviral production, the NF-κB reporter assay, and Statistics.
Lim J.T., Singh N., Leuvano L.A., Calvert V.S., Petricoin E.F., Teachey D.T., Lock R.B., Padi M., Kraft A.S, & Padi S.K. (2020). PIM kinase inhibitors block the growth of primary T-cell acute lymphoblastic leukemia: Resistance pathways identified by network modeling analysis. Molecular cancer therapeutics, 19(9), 1809-1821.
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3

Characterization of Myogenic Markers

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The following antibodies were used for immunofluorescence or western blot: pan-MyHC (MF20; DSHB), Titin (9D10; DSHB), MyHC-neo (N3.36; DSHB) (MHCN; Leica), MYH3 (F1.652; DSHB), MYH1/2 (SC-71; DSHB), α-actinin (MA122863; Thermofisher), Desmin (sc-23879; SCBT), ACTB (sc-4778; SCBT), OCT3/4 (C-10; SCBT), SOX2 (Y-17; SCBT), NANOG (H-2; SCBT), Alexa Fluor 488 Phalloidin (A12379; Thermofisher), Alexa fluor 555 goat anti-mouse IgG (A-21424; Thermo Fisher), Alexa fluor 488 goat anti-rabbit IgG (A-11008; Thermo Fisher), and mouse IgG HRP-linked (NA931; GE Healthcare).
Selvaraj S., Mondragon-Gonzalez R., Xu B., Magli A., Kim H., Lainé J., Kiley J., Mckee H., Rinaldi F., Aho J., Tabti N., Shen W, & Perlingeiro R.C. (2019). Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes. eLife, 8, e47970.
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4

Antibody Panel for Neural Cell Analysis

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The following antibodies were used for immunofluorescence (IF) and/or Western blot analysis: SMI31 – 1:1000 for WB and IF (Millipore, NE1022). MBP – 1:500 for WB and IF (Millipore, MAB382). β-catenin WB 1:500 (BD Biosciences, 610153). Active β-catenin (clone 8E7) (Millipore). α-bungarotoxin (Btx: to visualise NMJ, Invitrogen). F69-3G10 – 1:500 for WB (referred to as 3G10 in results) (Amsbio). F58-10E4 – 1:100 for IF (referred to as 10EA in results)(Amsbio). Sox2 – 1:1000 for Western blot (Abcam). WB secondary: Mouse IgG HRP-linked (GE Healthcare, NXA931). IF secondary: Alexa Fluor mouse IgG1, IgG2a (ThermoFischer Scientific, A-21121).
Whitehead M.J., McGonigal R., Willison H.J, & Barnett S.C. (2018). Heparanase attenuates axon degeneration following sciatic nerve transection. Scientific Reports, 8, 5219.
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