The SAA3–3′-UTR reporter plasmid, which contained the 5′-AATAAATACTTGTGAAATGCA-3′ sequence of 3′-UTR of SAA3, was purchased from GeneCopoeia. Primary-culture mouse chondrocytes were pretreated with hyaluronidase type I-S (Sigma) for 3 h in serum-free DMEM and transfected by incubation for 6 h with SAA3-3′-UTR reporter vector (0.05 μg) and Lipofectamine 2000, as described by the manufacturer. The cells were co-transfected with 0.05 μg of empty vector (Origene), WT-Regnase-1 expression vector (Origene), or D141N Regnase-1 (which lacks RNase activity) [13 (link)]. The cells were harvested at 24 h after treatment, and firefly luciferase and Renilla luciferase activities were measured using a Dual-Luciferase Assay System (Promega).
Saa3 3 utr reporter plasmid
Manufactured by GeneCopoeia
The SAA3–3′-UTR reporter plasmid is a laboratory tool used to study gene expression regulation. It contains the 3′-untranslated region (3′-UTR) of the SAA3 gene cloned downstream of a reporter gene. This plasmid can be used to investigate the regulatory role of the SAA3 3′-UTR in gene expression.
Automatically generated - may contain errors
2 protocols using saa3 3 utr reporter plasmid
1
Analyzing SAA3 3'UTR Regulation by Regnase-1
2
Regnase-1 Regulates SAA3 Expression
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The SAA3-3´-UTR reporter plasmid, which contained the 5´-AATAAATACTTGTGAAATGCA-3´ sequence of 3
´-UTR of SAA3, was purchased from GeneCopoeia. Primary-culture mouse chondrocytes were pretreated with hyaluronidase type I-S (Sigma) for 3 hours in serum-free DMEM, and transfected by incubation for 6 hours with SAA3-3´-UTR reporter vector (0.05 µg) and Lipofectamine 2000, as described by the manufacturer. The cells were co-transfected with 0.05 µg of empty vector (Origene), WT-Regnase-1 expression vector (Origene), or D141N Regnase-1 (which lacks RNase activity) [13] . The cells were harvested at 24 hours after treatment, and re y luciferase and Renilla luciferase activities were measured using a Dual-Luciferase Assay System (Promega).
´-UTR of SAA3, was purchased from GeneCopoeia. Primary-culture mouse chondrocytes were pretreated with hyaluronidase type I-S (Sigma) for 3 hours in serum-free DMEM, and transfected by incubation for 6 hours with SAA3-3´-UTR reporter vector (0.05 µg) and Lipofectamine 2000, as described by the manufacturer. The cells were co-transfected with 0.05 µg of empty vector (Origene), WT-Regnase-1 expression vector (Origene), or D141N Regnase-1 (which lacks RNase activity) [13] . The cells were harvested at 24 hours after treatment, and re y luciferase and Renilla luciferase activities were measured using a Dual-Luciferase Assay System (Promega).
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