Verikine hs mouse ifnβ elisa kit

The mouse IFNα platinum ELISA kit (normal sensitivity; Affymetrix eBioscience, Vienna, Austria) or the Verikine-HS mouse IFNα All subtype ELISA kit (high sensitivity; Pbl Assay Science, Piscataway, NJ, USA) was used to measure serum IFNα concentrations from LCMV infected mice. The Verikine mouse IFNβ ELISA kit (normal sensitivity; Pbl Assay Science) or the Verikine-HS mouse IFNβ ELISA kit (high sensitivity; Pbl Assay Science) was used to measure serum IFNβ concentrations from LCMV infected mice. These are standard ELISA kits that were performed according to manufacturer’s instructions. Alternatively, mouse IFNα and IFNβ were also measured using the mouse interferon dual plex (#EPX02A-22187-901, Thermofisher Scientific). A cytokine multiplex array was also performed using the ProcartaPlex™ 26-Plex Panel 1 (EPX01A-26088-901), according to manufacturer’s instructions (ProcartaPlex, Thermofisher Scientific). Plates were read using a Bioplex 200 analyser (Biorad, Hercules, CA, USA).

IL1β and IFNβ concentration in culture media of stable KD myoblasts was measured using the IL1β (mouse) ELISA kit (Sigma, RAB0274-1KT) and the VeriKine-HS (mouse) IFNβ ELISA Kit (PBL Assay Science, 42410-1), respectively, following the manufacturer’s instruction. Prior to the assay, stable KD myoblasts were plated in 10 cm² dishes and allowed growth until reaching 90% confluency. 10 mL of culture mediums were collected and concentrated down to 400 uL using the Vivaspin 6 centrifugal concentrators (Sigma, Z614483), following the manufacturer’s instructions.