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2 protocols using trimyristin

1

Obese Mouse Model: Trimyristin Treatment

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The obese mouse model was generated by feeding C57BL/6J male mice (Jackson Laboratory, Bar Harbor, ME, USA) with a high-fat diet (HFD) for 12 weeks starting from 8 weeks of age [11] , [12] (link). For the trimyristin treatment, obese mice were fed the HFD containing 5%(wt/wt) trimyristin (J62819, Alfa Aesar, Tewksbury, MA, USA) for two weeks. Glucose and insulin tolerance tests were performed as described previously [11] , [12] (link). All mouse studies were conducted in accordance with federal guidelines and were approved by the Institutional Animal Care and Use Committee of the University of California, Irvine.
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2

Nanoparticle Formulation Development

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Trilaurin (TL), tristearin, myristic acid, glyceryl monostearate, ethyl acetate (EA), benzyl alcohol (BenzOH), triacetin (TA), butyl lactate (BL), polysorbate (20, 40, 80), sodium taurodeoxycholate (Na TdC), sodium taurocholate (Na TC), sodium glycocholate (Na GC), sodium cholate (Na C), Pluronic® F68, polyvinyl alcohol (PVA) 9000, PVA 14000, 1,2 propanediol, curcumin and Sepharose® CL 4B were purchased from Sigma (Dorset, UK). Trimyristin was from Alfa Aesar (Karlsruhe, Germany), glyceryl dibehenate was from Gattefossé (Weil am Rhein, Germany), Epikuron® 200 (lecithin-phosphatydil coline 92%) from Cargill (Minneapolis, MN, USA), and Cremophor® RH 60 (PEG-60 hydrogenated castor oil) from BASF (Ludwigshafen, Germany).
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