A standard hematoxylin and eosin staining technique was used for histological analyses of paraffin-embedded (5-μm) skin sections. For immunohistochemistry, paraffin sections were deparaffinized with xylene and rehydrated with ethanol. Antigen unmasking was performed by boiling the slides in citrate-buffer (pH = 6, Dako, Santa Clara, CA) in a microwave for 5 minutes. After blocking the sections with 10% normal horse serum for 1 hour, sections were incubated with an unconjugated rabbit anti-human anti-HSV-1 serum (1:300; B01141, Dako) overnight at 4 °C. Subsequently, sections were incubated with a biotin-conjugated anti-rabbit IgG for 60 minutes at room temperature with the Elite rabbit IgG Vectastain kit (Vector Laboratories, Burlingame, CA). Biotinylated antibodies were detected with horseradish peroxidase-streptavidin, and staining was visualized with amino-ethyl-carbazole (Dako). Finally, sections were counterstained with hematoxylin (Merck, Darmstadt, Germany), mounted with Aquatex (Merck), and examined with a conventional bright field microscope (Olympus AX70; Olympus, Tokyo, Japan). Rabbit serum (Vector Laboratories) was used as isotype control.
B01141
Manufactured by Agilent Technologies
The B01141 is a laboratory equipment product from Agilent Technologies. It is designed to perform specific functions in a laboratory setting. The core function of this product is to provide reliable and accurate measurements for research and analysis purposes.
Automatically generated - may contain errors
Lab products found in correlation
Vectastain elite rabbit igg kit, by Vector Laboratories (2 mentions)
Hematoxylin, by Merck Group (2 mentions)
Aquatex, by Merck Group (2 mentions)
Citrate buffer, by Agilent Technologies (2 mentions)
Rabbit serum, by Vector Laboratories (2 mentions)
Aminoethylcarbazole, by Agilent Technologies (2 mentions)
2 protocols using b01141
1
Histological Analysis of Skin Sections
2
Histological Analysis of Skin Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A standard hematoxylin and eosin staining technique was used for histological analyses of paraffin-embedded (5-μm) skin sections. For immunohistochemistry, paraffin sections were deparaffinized with xylene and rehydrated with ethanol. Antigen unmasking was performed by boiling the slides in citrate-buffer (pH = 6, Dako, Santa Clara, CA) in a microwave for 5 minutes. After blocking the sections with 10% normal horse serum for 1 hour, sections were incubated with an unconjugated rabbit anti-human anti-HSV-1 serum (1:300; B01141, Dako) overnight at 4 °C. Subsequently, sections were incubated with a biotin-conjugated anti-rabbit IgG for 60 minutes at room temperature with the Elite rabbit IgG Vectastain kit (Vector Laboratories, Burlingame, CA). Biotinylated antibodies were detected with horseradish peroxidase-streptavidin, and staining was visualized with amino-ethyl-carbazole (Dako). Finally, sections were counterstained with hematoxylin (Merck, Darmstadt, Germany), mounted with Aquatex (Merck), and examined with a conventional bright field microscope (Olympus AX70; Olympus, Tokyo, Japan). Rabbit serum (Vector Laboratories) was used as isotype control.
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