Escherichia coli XL10-Gold and BL21(DE3) Gold cells were purchased from Stratagene. EZ-Run protein ladder was purchased from Fisher Bioreagents and Ni2+-nitrilotriacetate (Ni-NTA) resin from Qiagen. IANBD, Click-iT® Protein Reaction Buffer kit and iodoacetamide alkyne were obtained from Life Technologies. BADAN, oxazine derivatives (Blue Oxazine, Red Oxazine and Nile Blue) and Chromis dyes (Chromis 630 and 678) were purchased from Eurogentec, Chromeon and Cyanagen, respectively. The merocyanine dyes (Mero 53, 62 and 87) were synthesized as previously described [10 (link)–12 (link)]. Texas Red–dextran was obtained from Life Technologies.
Ni2 nitrilotriacetate ni nta resin
Manufactured by Qiagen
Ni2+-nitrilotriacetate (Ni-NTA) resin is a chromatography medium used for the purification of recombinant proteins with a histidine-tag. The resin consists of nickel-nitrilotriacetate chelated to an agarose support, which allows for the reversible binding of histidine-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Texas red dextran, by Thermo Fisher Scientific (2 mentions)
Iodoacetamide alkyne, by Thermo Fisher Scientific (2 mentions)
Ianbd, by Thermo Fisher Scientific (2 mentions)
Blue oxazine, by Eurogentec (2 mentions)
Red oxazine, by Eurogentec (2 mentions)
Nile blue, by Eurogentec (2 mentions)
Chromis dyes chromis 630 and 678, by Eurogentec (2 mentions)
Ez run protein ladder, by Thermo Fisher Scientific (2 mentions)
Click it protein reaction buffer kit, by Thermo Fisher Scientific (2 mentions)
2 protocols using ni2 nitrilotriacetate ni nta resin
1
Fluorescent Protein Labeling Strategies
2
Fluorescent Protein Labeling Strategies
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Escherichia coli XL10-Gold and BL21(DE3) Gold cells were purchased from Stratagene. EZ-Run protein ladder was purchased from Fisher Bioreagents and Ni2+-nitrilotriacetate (Ni-NTA) resin from Qiagen. IANBD, Click-iT® Protein Reaction Buffer kit and iodoacetamide alkyne were obtained from Life Technologies. BADAN, oxazine derivatives (Blue Oxazine, Red Oxazine and Nile Blue) and Chromis dyes (Chromis 630 and 678) were purchased from Eurogentec, Chromeon and Cyanagen, respectively. The merocyanine dyes (Mero 53, 62 and 87) were synthesized as previously described [10 (link)–12 (link)]. Texas Red–dextran was obtained from Life Technologies.
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