Optima HPLC grade methanol, Optima HPLC grade water, Optima HPLC grade acetonitrile, American Chemical Society (ACS) grade acetone, ACS grade methanol, ACS grade pentane, hydrochloric acid, ammonium acetate, dipotassium phosphate, monopotassium phosphate, magnesium chloride (MgCl2) and reduced glutathione (GSH) were purchased from Fisher Chemical (Fair Lawn, NJ, USA); NADPH, 1-aminobenzotriazole, methimazole, 1-naphthol and hydroxyacetone from Acros Organics (Morris Plain, NJ, USA); UDPGA, saccharolactone and 2,4-dichlorophenoxyacetic acid from Sigma-Aldrich (St. Louis, MO, USA); bupropion, benzydamine and alamethicin from Alfa Aesar (Ward Hill, MA, USA); oxcarbazepine from European Pharmacopoeia Reference Standard (Strasbourg, France); ticlopidine from Tokyo Chemical Industry (Tokyo, Japan); hydroxybupropion from Cerilliant Corporation (Round Rock, Texas, USA); deuterated acetone (acetone-d6) from Cambridge Isotope Labs (Cambridge, MA, USA); hydrogen peroxide (50%) from Univar (Redmond, WA, USA); HLM, rat liver microsomes (RLM), DLM and human lung microsomes (HLungM) from Sekisui XenoTech (Kansas City, KS, USA); human recombinant CYP (rCYP) bactosomes expressed in Escherichia coli (E. coli) from Cypex (Dundee, Scotland); human recombinant flavin monooxygenase (rFMO) supersomes and human recombinant UGT (rUGT) supersomes expressed in insect cells from Corning (Woburn, MA, USA).
Alamethicin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alamethicin is a natural product isolated from the fungus Trichoderma viride. It is a small, membrane-active peptide that forms voltage-dependent ion channels in lipid bilayers. Alamethicin has been used as a tool in biophysical and biochemical research to study the properties of ion channels and membrane transport processes.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Saccharolactone, by Merck Group (1 mentions)
Reduced glutathione, by Thermo Fisher Scientific (1 mentions)
Udpga, by Merck Group (1 mentions)
Acs grade methanol, by Thermo Fisher Scientific (1 mentions)
Ticlopidine, by Tokyo Chemical Industry (1 mentions)
Hydroxybupropion, by Cerilliant (1 mentions)
Dipotassium phosphate, by Thermo Fisher Scientific (1 mentions)
Monopotassium phosphate, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Thermo Fisher Scientific (1 mentions)
Optima hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Nadph, by Thermo Fisher Scientific (1 mentions)
2 4 dichlorophenoxyacetic acid, by Merck Group (1 mentions)
Acs grade acetone, by Thermo Fisher Scientific (1 mentions)
1 naphthol, by Thermo Fisher Scientific (1 mentions)
Digitonin, by Merck Group (1 mentions)
Abt 199, by Apexbio (1 mentions)
Anti cytochrome c antibody, by BD (1 mentions)
2 protocols using alamethicin
1
Integrated ADME Characterization Protocol
2
Apoptotic Priming Measurement by BH3 Profiling
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Apoptotic priming was measured as the depletion of intracellular cytochrome C using flow cytometry-based BH3 profiling as described by Ryan et al.19 (link) Briefly, 106 cells/ml in complete media containing 25 ng/ml IL7 were treated with vehicle, 1 μm dexamethasone and/or 500 nm ruxolitinib for 16 h, permeabilized with digitonin (Sigma-Aldrich), exposed to 100 nm BIM BH3 peptide (New England Biosciences, Ipswich, MA, USA) or 1 μm ABT-199 (ApexBio, Houston, TX, USA) for 1 h, fixed with 4% paraformaldehyde, stained with anti-cytochrome c antibody (Cat# 558709, BD Biosciences), and subjected to flow cytometry. Cells treated with DMSO and alamethicin (Alfa Aesar, Haverhill, MA, USA) were used as negative and positive controls respectively. For each condition, the percentage of cytochrome c depletion was calculated based on the release of cytochrome c in the DMSO (0% loss) and alamethicin (100% loss) controls and then normalized to vehicle treated cells.
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