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Legend max human il 23 elisa kit

Manufactured by BioLegend

The LEGEND MAX Human IL-23 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-23 (IL-23) in cell culture supernatants, serum, and plasma samples.

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2 protocols using legend max human il 23 elisa kit

1

Quantifying Serum Cytokines and Antibodies

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Human serum concentrations of CXCL9, CXCL10, and CXCL11 were measured using the LEGENDplex Human Proinflammatory Chemokine Panel (BioLegend) according to the manufacturer’s instructions. Human serum concentrations of other cytokines were measured using the 42-Plex Human Cytokine/Chemokine Array (Eve Technologies). Mouse serum antibody concentrations were measured using the LEGENDplex Mouse Immunoglobulin Isotyping Panel (BioLegend). Mouse serum cytokine and chemokine concentrations were measured using the LEGENDplex Mouse Inflammation Panel (BioLegend). Cytokine concentrations from cell culture supernatant were measured with the Mouse IL-12 (p70) ELISA MAX Deluxe, Human IL-12 (p70) ELISA MAX Deluxe, and LEGEND MAX Human IL-23 ELISA Kit (all from BioLegend).
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2

Quantification of IL-27 and IL-23 in Serum

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The serum of blood samples was collected from each subject, and the concentration of IL-27 was quantified by using a LEGEND MAX™ Human IL-27 ELISA kit (BioLegend, San Diego, CA). The concentration of IL-23 was detected by using a LEGEND MAX™ Human IL-23 ELISA kit (BioLegend, San Diego, CA). The protocol was performed according to the manufacturer's protocols. The sensitivity of the ELISA kits was 11 pg/ml for IL-27 and 2.45 pg/ml for IL-23.
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