Anti dykddddk hrp conjugate antibody

KOD-plus Neo kit was obtained from Toyobo (Osaka, Japan). Infusion enzyme was obtained from TaKaRa (Tokyo, Japan). E. coli SHuffle T7 Express lysY were obtained from New England Biolabs Korea (Seoul, Korea). A plasmid miniprep kit and oligonucleotides were obtained from Bionics (Daejeon, Korea). His Sepharose Ni was obtained from GE healthcare (Piscataway, NJ, USA). The Nanosep Centrifugal-3 k Ultrafiltration Device was obtained from Pall Corporation (Ann Arbor, MI, USA). Maxi plates were obtained from SPL Life Sciences (Gyeonggi-do, Korea). Anti-DYKDDDDK-HRP conjugate antibody was obtained from (Biolegend, CA, USA) and 3,3′,5,5′-Tetramethylbenzidine (TMB) was obtained from Sigma (St. Louis, MO, USA). Purified MMP9 protein was obtained from Sino (Beijing, China). Purified catalytic domain of MMP9 was obtained from Abcam (Cambridge, United Kingdom). Other chemicals and reagents, unless otherwise indicated, were from Sigma (Seoul, Korea).

Chemical genes were obtained from Lncbio (Seoul, Korea). Oligonucleotides and the plasmid miniprep kits were obtained from Bionics (Seoul, Korea). KOD-One DNA polymerase and the In-Fusion HD cloning kit were from Takara-Bio (Tokyo, Japan). E. coli SHuffle T7 Express lysY were obtained from New England Biolabs (Seoul, Korea). Talon beads were obtained from Clontech (Mountain View, CA, USA). The gravity empty column was from Bio-Rad (Daejeon, Korea). Ultrafiltration devices were obtained from Pall (Nanosep Centrifugal-3 k; Ann Arbor, MI, USA). Anti-DYKDDDDK-HRP conjugate antibody was obtained from (Biolegend, CA, USA). Recombinant mouse PD-L1 protein was obtained from Sino Biological Inc. (Beijing, China). Spin-type ultrafiltration devices were obtained from Millipore (Bedford, MA, USA). Maxi plates were obtained from SPL Life Sciences (Gyeonggi-do, Korea). HRP-conjugated anti-Flag antibody was obtained from Biolegend (San Diego, CA, USA). Other chemicals and reagents, unless otherwise indicated, were from Sigma (Seoul, Korea).