Plasma levels of mouse insulin and leptin were measured by sandwich ELISA using ALPCO's Mouse Ultrasensitive Insulin ELISA (Salem, NH) and Millipore's Human Leptin “Dual Range” ELISA (EMD Millipore, Billerica, MA) according to manufacturer's instructions. The leptin ELISA was adapted to measure tissue leptin levels using supernatant from tissue samples homogenized with a TissueLyser in RIPA buffer containing 1% Triton X-100. The lysate was used in place of plasma in the ELISA kit, leptin concentrations normalized to tissue protein content, and results were compared to serially diluted human adipose lysate samples assayed in parallel.
Millipore s human leptin dual range elisa
Manufactured by Merck Group
Millipore's Human Leptin "Dual Range" ELISA is a laboratory equipment product designed for the quantitative measurement of human leptin levels in biological samples. The product utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify leptin, a hormone involved in regulating energy balance and body weight.
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Lab products found in correlation
2 protocols using millipore s human leptin dual range elisa
1
Measuring Mouse Insulin and Leptin
2
Measuring Mouse Insulin and Leptin
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