Hpo rnai

Manufactured by Vienna Drosophila Resource Center

The Hpo RNAi is a laboratory tool used to study gene function in Drosophila. It is a double-stranded RNA molecule that can be used to knockdown the expression of the Hippo (Hpo) gene in Drosophila cells or tissues. The Hpo gene is a key regulator of cell growth and proliferation, and its knockdown can be used to investigate the role of the Hippo signaling pathway in various biological processes.

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Lab products found in correlation

Atg1 rnai, by Bloomington Drosophila Stock Center (1 mentions) En gal4, by Bloomington Drosophila Stock Center (1 mentions) Sev gal4, by Bloomington Drosophila Stock Center (1 mentions) Hml gal4, by Bloomington Drosophila Stock Center (1 mentions) Ban lacz, by Bloomington Drosophila Stock Center (1 mentions) W1118, by Vienna Drosophila Resource Center (1 mentions)

2 protocols using hpo rnai

Drosophila Genetics Techniques Protocol

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Stocks were raised on standard cornmeal-agar medium. Stag 17 embryos, 3rd instar larvae, 3- to 6-days old adults were used in this study. w1118 flies were used as a standard wild-type strain. Hml-Gal4 (stock ID 30141), Hml > GFP (stock ID 30142), en-Gal4 (stock ID 83350), sev-Gal4 (stock ID 5793), UAS-Tak1 (stock ID 58810), Tak1² (stock ID 26272), Atg1 RNAi (stock ID 26731), Atg101 RNAi (stock ID 34360) and ban-lacZ (stock ID 10154) lines were obtained from Bloomington Drosophila Stock Center (BDSC). Tak1 RNAi (stock ID 101357), Tao-1 RNAi (stock ID 17432), Hpo RNAi (stock ID 104169), Wts RNAi (stock ID 106174), Atg13 RNAi (stock ID 27955) and Yki RNAi (stock ID 40497) lines were collected from Vienna Drosophila Resource Center (VDRC). Atg9 RNAi (stock ID THU2895) was collected from TsingHua Fly Center. UAS-Yki line has been reported previously^{85 (link)}.

Yang Y., Zhou H., Huang X., Wu C., Zheng K., Deng J., Zheng Y., Wang J., Chi X., Ma X., Pan H., Shen R., Pan D, & Liu B. (2024). Innate immune and proinflammatory signals activate the Hippo pathway via a Tak1-STRIPAK-Tao axis. Nature Communications, 15, 145.

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Genetic manipulation of Drosophila to study hippo

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w¹¹¹⁸ (#60000), control RNAi (#60100) and hpo RNAi (#104169) were purchased from the Vienna Drosophila Resource Center (Vienna, Austria). The appl-GAL4 (#32040), elav-GAL4 (#458), elav-GS-GAL4 (#43642) and UAS-hpo (#44254) strains were purchased from the Bloomington Stock Center (Indiana University, Bloomington, IN). The UAS-mt-Keima line was previously generated^{31 (link)}. The following genotypes were used: appl>mt-keima, control RNAi (appl-GAL4/Y; UAS-mt-keima/+); appl>mt-keima, hpo RNAi (appl-GAL4/Y; UAS-mt-keima/UAS-hpo^KK101704); appl >mt-keima, w¹¹¹⁸(appl-GAL4/Y; UAS-mt-keima/+); appl>mt-keima, hpo (appl-GAL4/Y; UAS-mt-keima/+; UAS-hpo/+); elav>control RNAi (elav-GAL4/Y); elav>hpo RNAi (elav-GAL4/Y; UAS-hpo^KK101704/+); elav-GS>hpo (elav-GS-GAL4/UAS-hpo); TH > mitoGFP, w¹¹¹⁸(TH-GAL4, UAS-mitoGFP/+); and TH > mitoGFP, hpo (TH-GAL4, UAS-mitoGFP/UAS-hpo).

Jeong D.J., Um J.H., Kim Y.Y., Shin D.J., Im S., Lee K.M., Lee Y.H., Lim D.S., Kim D, & Yun J. (2024). The Mst1/2-BNIP3 axis is required for mitophagy induction and neuronal viability under mitochondrial stress. Experimental & Molecular Medicine, 56(3), 674-685.

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