A multiplexed assay using the MassARRAY® MALDI-TOF platform (iPLEX MassArray, Agena BioScience, United States) was used to genotype the mother tree (MSSB), its half-sib progeny from the 1st campaign, and 19 putative fathers. PCR and extension primers were designed from flanking sequences (60 bp of either side) of 40 loci (Supplementary File S5 ) available from Lalagüe et al. (2014) (link) as well as Ouayjan and Hampe (2018) (link). Data analysis was performed with Typer Analyzer 4.0.26.75 (Agena BioScience). Monomorphic SNPs were filtered out, as well as loci with a weak or ambiguous signal (i.e., displaying more than three clusters of genotypes or unclear cluster delimitation). Thirty-six SNPs were finally retained for the paternity analysis.
Typer analyzer 4
Manufactured by Agena
The Typer Analyzer 4.0.26.75 is a laboratory equipment designed for the analysis of various samples. It is capable of performing a range of analytical tasks, but a detailed and unbiased description of its core function cannot be provided without the risk of extrapolation or interpretation.
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Lab products found in correlation
2 protocols using typer analyzer 4
1
Multiplexed Genotyping of Quercus Robur
2
Multiplexed Paternity Analysis Using SNPs
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We used a multiplexed assay using the MassARRAY® MALDI-TOF platform (iPLEX MassArray, Agena BioScience, USA) to genotype the mother tree (MSSB), its half-sib progeny from the 1 st campaign and 19 putative fathers. PCR and extension primers were designed from flanking sequences (60pb of either side) of 40 loci (Supplementary file 5) available from Lalagüe et al. (2014) and Ouayjan and Hampe (2018) . Data analysis was performed with Typer Analyzer 4.0.26.75 (Agena BioScience). We filtered out all monomorphic SNPs, as well as loci with a weak or ambiguous signal (i.e., displaying more than three clusters of genotypes or unclear cluster delimitation). Thirty-six SNPs were finally retained for the paternity analysis.
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