Anti k10 prb 159p

Biopsies were fixed for 1 hour in cold 3.7% paraformaldehyde, incubated overnight in 30% sucrose at 4 degrees, before embedding in OCT. Frozen sections were stained using standard protocols with the following antibodies: anti-K17 (D73C7, 1:1,500, Cell Signaling); anti-K8 (TROMA-I, 1:500, Developmental Studies Hybridoma Bank); anti-K20 (D409, 1:500, American Research Products); anti-K14 (AF64, 1:1,000,000, Covance); anti-K5 (03-GP-CK5, American Research Products); anti-GFP/YFP (GFP-1020, 1:2,000, Aves Labs); anti-NF (C28E10, 1:500, Cell Signaling); anti-β4 (346-11A, 1:500, BD Pharmingen); anti-Sox9 (H-90, 1:150, Santa Cruz Biotechnology); anti-Lrig1 (AF3688, 1:25, R&D Systems); anti-K10 (PRB-159P, 1:500, Covance); anti-Involucrin (PRB-140C, 1:500, Covance); anti-Chromogranin A (24-1113C1, 1:250, American Research Products); and anti-CD200 eFluor660 (OX90, 1:2,000, eBioscience). For frozen samples stained for YFP, sections were pre-treated with cold methanol for 5 minutes prior to blocking. For whole-mount β-gal staining, in situ staining and qPCR, see Supplemental Information. Image processing was performed using Adobe Photoshop CS6, with the Auto-Blend function applied to maximize image sharpness across focal planes in the same microscopic field.