SU-DHL-4 (CRL-2957), NU-DUL-1 (CRL-2969), and SU-DHL-8 (CRL-2961) cell lines were purchased from the Non-Hodgkin’s Lymphoma Cell Line Panel at ATCC (Manassas, VA, USA) and maintained in RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific). All cell lines were cultured at 37°C under 5% CO2. Media was refreshed every 48 h.
Nudul 1
Manufactured by American Type Culture Collection
Sourced in United States
The NUDUL-1 is a laboratory equipment designed for the cultivation and maintenance of human and animal cell lines. It provides a controlled environment for optimal cell growth and proliferation. The NUDUL-1 features precise temperature, humidity, and gas concentration regulation to mimic the natural conditions required for cell cultivation.
Automatically generated - may contain errors
Lab products found in correlation
Su dhl 4, by American Type Culture Collection (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Su dhl 6, by American Type Culture Collection (2 mentions)
Su dhl 2, by American Type Culture Collection (2 mentions)
Su dhl 8, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Jvm 2, by American Type Culture Collection (1 mentions)
Jeko 1, by American Type Culture Collection (1 mentions)
Rec 1, by American Type Culture Collection (1 mentions)
Pfeiffer, by American Type Culture Collection (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hepes, by Corning (1 mentions)
Advanced rpmi, by Thermo Fisher Scientific (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Dohh2, by American Type Culture Collection (1 mentions)
Oci ly 19, by American Type Culture Collection (1 mentions)
8 protocols using nudul 1
1
Cell Line Maintenance Protocol
2
Establishing Cell Lines for CD74 Targeted Therapy
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SU-DHL-6, SU-DHL-2, SU-DHL-4, Pfeiffer, NUDUL-1, HT, JVM-2, Jeko-1, Mino, Rec-1 and A20 cells were purchased from ATCC (American Type Culture Collection, Manassas, VA). OCI-Ly3, U-2932, RIVA, WSU-DLCL2, WSU-NHL, WSU-FSCCL, OCI-Ly1, OCI-Ly19 and OPM2 cells were purchased from The Leibniz Institute DSMZ (German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany). All the cell lines were maintained in RPMI, high glucose (Corning, Corning, NY, USA) supplemented with 20% heat-inactivated fetal bovine serum (Thermo Scientific, Grand Island, NY, USA), 2 mM GlutaMax (Thermo Scientific, Grand Island, NY, USA), and 1× penicillin/streptomycin (Corning, Corning, NY, USA).
To evaluate the cross-reactivity of STRO-001, CHO-k cells were purchased from ATCC and CHO-humanCD74 and CHO-cynoCD74 stable cell lines were generated by transfecting CHO-k cells with a plasmid containing human or cynomolgus monkey CD74 cDNA sequences. CHO-humanCD74, CHO-cynoCD74 stable cells, CHO-k were maintained in RPMI, high glucose (Corning, Corning, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Thermo Scientific, Grand Island, NY, USA), 2 mM GlutaMax (Thermo Scientific, Grand Island, NY, USA), and 1× penicillin/streptomycin (Corning, Corning, NY, USA).
To evaluate the cross-reactivity of STRO-001, CHO-k cells were purchased from ATCC and CHO-humanCD74 and CHO-cynoCD74 stable cell lines were generated by transfecting CHO-k cells with a plasmid containing human or cynomolgus monkey CD74 cDNA sequences. CHO-humanCD74, CHO-cynoCD74 stable cells, CHO-k were maintained in RPMI, high glucose (Corning, Corning, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Thermo Scientific, Grand Island, NY, USA), 2 mM GlutaMax (Thermo Scientific, Grand Island, NY, USA), and 1× penicillin/streptomycin (Corning, Corning, NY, USA).
3
Cell Line Maintenance Protocol for GCB-DLBCL
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NUDUL1 was from ATCC CRL-2969, OCI-Ly8 was from NCI and Dogkit was from DMSZ. GCB-DLBCL cell lines were grown at 37 °C with 5% CO 2 in Advanced RPMI (Invitrogen) supplemented with 4% fetal bovine serum (Tet tested, Atlanta Biologics), 1% pen/strep (Mediatech), 10 mM HEPES, pH 7.2-7.6 (Corning) and 2 mM l-glutamine (Mediatech). The 293FT (Thermo) and PLAT-E (a gift from S. Schwab) cells were grown in DMEM (Invitrogen) supplemented with 10% fetal bovine serum, 1% pen/strep and 1% l-glutamine. Cell lines were regularly tested for Mycoplasma using the MycoAlert Mycoplasma Detection kit (Lonza).
4
Culturing 16 DLBCL Cell Lines
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The 16 DLBCL cell lines (DOHH2, HT, OCI-LY19, DB, OCI-LY1, SUDHL-4, SUDHL-5, SUDHL-10, NUDHL-1, OCI-LY7, WSU-DLCL2, SUDHL-6, NUDUL-1, U2932, OCI-LY3, and RI-1) were purchased from the American Type Culture Collection or from DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany). They were grown in RPMI-1640 Glutamax medium (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 10% fetal bovine serum (FBS) (PAA laboratory GmbH, Pasching, Austria) (U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, and WSU-DLCL2 cells), 20% FBS (OCI-LY3, DB, SUDHL-5, NUDHL-1, and SUDHL-6 cells), or 15% FBS (NUDUL-1 cells). OCI-LY1, and OCI-LY7 cells were cultured in IMDM Glutamax (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 20% FBS, and OCI-LY19 cells in MEM alpha modified Glutamax (Gibco, Invitrogen, Cergy Pontoise, France) with 20% FBS. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO2. Contamination by Mycoplasma species was regularly monitored.
5
Cell Line Authentication and Mycoplasma Detection
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NuDUL1, SuDHL-2 and SuDHL-4 cells were purchased from the American Type Culture Collection (ATCC, USA). OCI-LY1, OCI-LY8 and OCI-LY10 were kindly provided by Dr. B.
Hilda Ye (Albert Einstein College of Medicine, NY, USA), and the DoHH2 cells were a gift from Prof. Mingzhi Zhang (the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China). Toledo cells were provided as a gift from Dr. Thomas Wu (Sundia MediTech, Shanghai, China). All cell lines were authenticated through short tandem repeat profiling at SIBS (Shanghai, China) and tested for mycoplasma infection using the Universal Mycoplasma Detection Kit (ATCC). Generation of cells stably transfected with lentivirus was performed (supplementary material, Supplementary materials and methods).
Hilda Ye (Albert Einstein College of Medicine, NY, USA), and the DoHH2 cells were a gift from Prof. Mingzhi Zhang (the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China). Toledo cells were provided as a gift from Dr. Thomas Wu (Sundia MediTech, Shanghai, China). All cell lines were authenticated through short tandem repeat profiling at SIBS (Shanghai, China) and tested for mycoplasma infection using the Universal Mycoplasma Detection Kit (ATCC). Generation of cells stably transfected with lentivirus was performed (supplementary material, Supplementary materials and methods).
6
DLBCL Cell Lines Response to CHOP and PD-1 Treatment
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Human DLBCL cell lines (U2932, OCI‐LY3, and SU‐DHL‐3) and one normal human lymphoblastoid cell line (GM12878) were purchased from BeNa Culture Collection (Suzhou, China). NU‐DUL‐1 was obtained from the American Type Culture Collection (ATCC, USA). Cells were cultured in RPMI 1640 medium (Gibco) replenished with 15% FBS (Gibco) and 1% penicillin/streptomycin in a standard cell incubator at 37°C with 5% CO2.
For CHOP stimulation, CHOP (Sigma‐Aldrich) at indicated concentrations (10, 20, 80, 160, 320 ng/ml) was directly added into the cell culture medium for 2 days. For rPD‐1 treatment, cultured cells were treated with human recombinant PD‐1 (R&D Systems, Minneapolis, USA, 8 μg/ml) for 24 h followed by CHOP treatment for an additional 2 days.
For CHOP stimulation, CHOP (Sigma‐Aldrich) at indicated concentrations (10, 20, 80, 160, 320 ng/ml) was directly added into the cell culture medium for 2 days. For rPD‐1 treatment, cultured cells were treated with human recombinant PD‐1 (R&D Systems, Minneapolis, USA, 8 μg/ml) for 24 h followed by CHOP treatment for an additional 2 days.
7
Doxorubicin-induced Cell Stress Model
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The H9c2 cells, HEK293T cells and diffuse large B-cell lymphoma (DLBCL) cell lines (NUDUL-1 and TMD8) were all purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). H9c2 cells and HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM, HyClone, Logan, USA) containing with 10% fetal serum (FBS, Gibco, Gaithersburg, USA) and 1% penicillin-streptomycin solution. NUDUL-1 and TMD8 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco). miR-25 mimic/NC mimic, miR-25 inhibitor/NC inhibitor and PTEN siRNA/NC siRNA were synthesized by Ribobio Technology Co, Ltd (Guangzhou, China). After seeded into plates at indicated density, cells were transfected with these agents for 24-48 hours by Lipofectamine 2000 (Invitrogen, CA, USA) referring the protocol of manufacture. The transfection efficacy was determined by RT-PCR or Western blot. After transfection, cells were exposed to 5μM DOX for 24 hours and harvested for further experiments. The primer of siRNA for PTEN is as follow: GGCTAAGTGAAGACGACAA.
8
Cultivation of DLBCL cell lines
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DLBCL cell lines SUDHL-4 (SUD, GCB subtype), OCI-LY8 (LY8, GCB subtype), and NU-DUL-1 (DUL, ABC subtype) and 293T cells were purchased from the American Type Culture Collection (ATCC). LY8 cells were cultured in Iscove modified Dulbecco's medium (HyClone) containing 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin. SUD and DUL cells and 293T cells were cultured in RPMI-1640 medium, containing 10% FBS and 1% penicillin/streptomycin (HyClone), at 37°C under 5% CO2. When necessary, 1% antibiotics (penicillin, streptomycin, amphotericin) (Gibco) were added to the medium to maintain the culture. Cells were passaged every 2-3 days.
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