BV2 microglia were purchased from the American Type Culture Collection and cultured in T25 flasks using DMEM medium (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA) at 37°C. The cell passages were performed when the cell density reached 80%-90% confluency.
Bv2 microglia
Manufactured by American Type Culture Collection
Sourced in United States
The BV2 microglia is a cell line derived from primary mouse microglia. It is widely used in research as a model for studying the functions and behaviors of microglia, the resident immune cells of the central nervous system.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Raw264.7 macrophage, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Hcc1806, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
Murine raw264.7 macrophages, by American Type Culture Collection (1 mentions)
5 protocols using bv2 microglia
1
BV2 Microglia Cell Culture Protocol
2
Culture of RAW264.7 and BV2 cells
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RAW264.7 macrophages and BV2 microglia were purchased from American Type Culture Collection (ATCC). RAW264.7 and BV2 cells were cultured in DMEM medium (supplemented with 10% heat-inactivated FBS, penicillin (100 units/mL), streptomycin (100 mg/mL) and L-glutamine (2 mM)) and were grown at 37 °C in a humidified atmosphere containing 5% CO2
3
Genetically Modified BV2 Microglia Assay
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Immortalised mouse BV2 microglia were purchased from the American Type Culture Collection (ATCC, Manassas, VA). The cells were cultured in DMEM (Thermo Fisher Scientific, Waltham, MA) medium supplemented with 10% foetal bovine serum (Gibco) and 100 U/ml penicillin and streptomycin at 37°C and 5% CO2. NEK7 small interfering RNA (siRNA) was used to knock down NEK7 in BV2 cells, and pcDNA3.1 was used to overexpress TLR4 and NLRP3 in BV2 cells. BV2 cells were transfected with control siRNA, NEK7 siRNA, pcDNA3-TLR4 or pcDNA3.1-NLRP3 following the manufacturer’s instructions. The efficiency of knockdown or overexpression of BV2 cells was confirmed by reverse transcription–quantitative polymerase chain reaction (RT-qPCR) and Western blotting.
4
Culturing Breast Cancer Cell Lines
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Breast cancer cell lines MCF-7, T47D, HCC1937, HCC1806, MDA-MB-231, and SKB-R3, along with BV-2 microglia cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), and incubated in the incubator (WCI-180, Wiggens, Germany) with a humid atmosphere of 37 °C and 5% CO2. All cell culture reagents were obtained from Thermo Fisher Scientific (Waltham, MA, USA).
5
Macrophage-Epithelial Cell Coculture for PM₁₀ Exposure
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Murine RAW 264.7 macrophages, LA-4 lung epithelial cells, and BV-2 microglia were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). In a RAW264.7/LA-4 coculture system, LA-4 cells were seeded until they completely adhered. After 2 days, RAW264.7 cells were plated at a ratio of 1:10 (RAW264.7:LA-4). The next day, 50 μg/mL of PM10 was applied to the cocultured LA-4/RAW264.7 cells for 24 h. These media were applied to BV2 to induce microglial activation.
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