Sca casa system

Manufactured by Microptic

Sourced in Spain

The SCA CASA System is a specialized lab equipment designed for the analysis of semen samples. The system provides automated and objective assessment of sperm concentration, motility, and morphology to support clinical andrology and assisted reproductive technology (ART) laboratories.

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Lab products found in correlation

Sperm class analyser, by Microptic (1 mentions)

5 protocols using sca casa system

Semen Analysis Protocol for Infertility

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The volunteers had been instructed to keep abstinence time between 2 to 7 days, and their abstinence time was recorded on the questionnaire. Semen samples were collected by masturbation into a wide-mouth plastic container at a private room. Then it was incubated at 37°C for liquefaction. An experienced technician performed semen analysis in accordance with the World Health Organization 2010 guideline [15 ]. Semen volume was assessed by weighing semen sample assuming 1g = 1ml. Sperm morphology was identified by sperm smears using Diff-Quick staining (Bred life science, Product code: BRED-015). Sperm concentration and motility were assessed by computer-aided sperm analysis (SCA CASA System; Microptic S.L., Barcelona, Spain). Sperm motility was classified as progressive motility and total motility.

Wang X., Zou P., Mo M., Yang H., Chen Q., Zhou N., Sun L., Chen H., Ao L., Cui Z, & Cao J. (2018). Early pubertal timing is associated with lower sperm concentration in college students. Oncotarget, 9(36), 24178-24186.

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Computer-Assisted Sperm Analysis Protocol

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In all cases, care was taken to avoid sperm contact with water and prevent exposure to direct sunlight and/ or temperatures outside the range of 19-21 °C. Immediately after collection, fresh semen characteristics were determined using a Sperm Class Analyser -Computer Assisted Sperm Analysis (SCA CASA) system (MICROPTIC S.L., Barcelona, Spain), with concentration and motility determination modules and a Nikon eclipse E200LED MV R camera equipped with a Nikon 10 × 0.25 Ph 1 BM WD 7.0 lens (NICON CORPORATION, Tokyo, Japan) and an acA 1300-200uc Basler c-Mount camera (BASLER AG, Ahrensburg, Germany). Semen concentration (× 10 6 /ml) and total motility parameters were measured in a counting chamber Leja SC 20-01-08-B-CE (CRYO TECH s.r.o., Liběchov, Czech Republic) of 8 × 2 µl volume. The fresh semen characteristics assessed included percentage of spermatozoa showing progressive and non-progressive motility and immotility, the velocity percentage of spermatozoa with rapid (> 100 µm/s), medium (50 µm/s) and slow (< 10 µm/s) motility and the percentage of rapid and medium progressive and non-progressive spermatozoa. Progressive motility refers to spermatozoa that are mostly swimming in a straight line or in very large circles, while non-progressive motility refers to spermatozoa that move but do not make forward progression in straight lines or by swimming in very tight circles.

Piaček V., Zukal J., Vetešník L., Heger T., Němcová M., Přibyl M., Vitula F., & Pikula J. (2020). Fresh semen characteristics in captive accipitrid and falconid birds of prey. Acta Veterinaria Brno, 89(3), 291-300.

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Sperm Collection and Analysis

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Sperm was collected as previously described [16 (link)], with slight modifications. Cauda epididymis was punctured followed by incubation in 400 µL human tubal fluid (HTF, Merck KGaA, Darmstadt, Germany) at 37 °C for 10 min. Next, computer-aided sperm analysis (SCA CASA System; Microptic S.L., Barcelona, Spain) was used for sperm concentration and motility assessment.

Zou P., Wang X., Yang W., Liu C., Chen Q., Yang H., Zhou N., Zeng Y., Chen H., Zhang G., Liu J., Cao J., Ao L, & Sun L. (2019). Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). International Journal of Molecular Sciences, 20(18), 4470.

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Assessing Male Reproductive Parameters

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Subjects were instructed to keep abstinence time between 2 and 7 days before participating in the survey. Semen samples were collected by masturbation in a private room, and subsequently incubated at 37°C for liquefaction. Semen volume was determined by weighting assuming 1g=1ml. Smears for morphology were measured using Diff-Quick staining (Bred life science, China, Product Code: BRED-015). Sperm concentration and motility were measured by computer-aided sperm analysis (SCA CASA System; Microptic S.L., Barcelona, Spain). The detailed procedure was in accordance with the instruction of the analysis system. Total motility equals progressive motility plus nonprogressive motility. Total sperm number = semen volume × sperm concentration. Total motile sperm count = total sperm number × total motility. In order to reduce the variation in assessment of semen characteristics, all semen samples were analyzed by one well-trained technician throughout the survey, who was well trained in semen analysis under the guidance of the Chongqing Science and Technology Commission.

Zou P., Wang X., Chen Q., Yang H., Zhou N., Sun L., Chen H., Liu J., Ao L., Cui Z, & Cao J. (2019). Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China. BioMed Research International, 2019, 5129263.

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Semen Analysis: Standardized Methodology

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Semen samples were collected by masturbation into a sterile, wide-mouth plastic container in an independent clean room. Then, the samples were incubated at 37°C for liquidation and were analyzed within 60 min. Semen volumes were measured by weighing, assuming that 1 ml of volume equals 1 g of weight. The semen parameters (motility, progressive motility, concentration, and total sperm count) were assessed with a computer-aided sperm analysis system (SCA CASA System; Microptic S.L., Barcelona, Spain) by a well-trained laboratory technician. Sperm morphology was identified by sperm smears using a Diff-Quick staining kit (Boruide, BRED-015). All semen analyses were performed according to the WHO criteria recommendations (26 ).

Cui Z., Mo M., Chen Q., Wang X., Yang H., Zhou N., Sun L., Liu J., Ao L, & Cao J. (2021). Pornography Use Could Lead to Addiction and Was Associated With Reproductive Hormone Levels and Semen Quality: A Report From the MARHCS Study in China. Frontiers in Endocrinology, 12, 736384.

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