Ginsenoside Rh3 ([HPLC] ≥ 98%) was obtained from the Biomedical Research Institute of Northwest University (Xi’an, Shaanxi, China—lot number: 20,210,723; validity period extending until July 2023). RPMI 1640 was purchased from HyClone (Logan, UT, USA). Methylthiazolyldiphenyl tetrazolium bromide (MTT) and cobalt chloride (CoCl2) were purchased from Beyotime Biotechnology (Shanghai, China). Trypsin, penicillin–streptomycin (pen–strep), and crystal violet were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Biological Industries (Cromwell, CT, USA). U0126 was purchased from MedChemExpress. Bouin’s solution was purchased from Phygene (Fuzhou, Fujian, China). The list of antibodies used is shown in Supplementary Table S1 .
Penicillin streptomycin pen strep
Manufactured by Solarbio
Sourced in China
Penicillin–streptomycin (pen–strep) is a sterile solution of antibiotics commonly used in cell culture applications. It contains the antibiotics penicillin and streptomycin, which work to inhibit the growth of various bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Methylthiazolyldiphenyl tetrazolium bromide mtt, by Beyotime (1 mentions)
Bouin s solution, by Phygene (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Trypsin, by Solarbio (1 mentions)
Crystal violet, by Solarbio (1 mentions)
U0126, by MedChemExpress (1 mentions)
Mitoq, by Focus Biomolecules (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin edta 0.25, by Thermo Fisher Scientific (1 mentions)
Light cycler 480 sybr green 1 master mix, by Takara Bio (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
2 protocols using penicillin streptomycin pen strep
1
Ginsenoside Rh3 Cellular Effects
2
Leptin-induced Oxidative Stress in BEAS-2B Cells
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BEAS-2B cells (no. ATCC® CRL-9609™) were obtained from the American Type Culture Collection and were tested negative for mycoplasma. DMEM-high glucose, FBS and Trypsin-0.25% EDTA were from Gibco (Thermo Fisher Scientific, Inc.). Recombinant human leptin was purchased from Novoprotein Scientific Inc. Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (Merck KGaA). mitoQ was purchased from Focus Biomolecules. DMSO and penicillin-streptomycin (pen-strep) were obtained from Solarbio. The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies. MitoSOX™ Red mitochondrial superoxide indicator for live-cell imaging (cat. no. M36008), MitoTracker® Red CM-H2XRos (cat. no. M7513) and TRIzol® reagent were purchased from Invitrogen (Thermo Fisher Scientific, Inc.). DAPI staining solution was obtained from Beyotime Institute of Biotechnology, Inc. PrimeScript™ RT Master Mix (cat. no. RR036A) was obtained from Takara Bio, Inc. LightCycler® 480 SYBR-Green I Master Mix (cat. no. 04707516001) was purchased from Roche Diagnostics. ELISA kit (cat. no. ml058059; Shanghai Enzyme-linked Biotechnology Co., Ltd.) and all other chemicals of the highest purity available were purchased from local companies.
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