Ad5-yCD/mutTKSR39rep-mIL12 has been described earlier.13 (link) In brief, it is a 55 kDa E1B-deleted replication-competent adenovirus that contains a yeast cytosine deaminase (yCD)/mutant SR39 herpes simplex virus thymidine kinase (mutTKSR39) fusion gene in the E1 region and a single-chain murine IL-12 gene in the E3 region. Both the yCD/mutTKSR39 fusion gene and IL-12 genes are under the transcriptional control of the human cytomegalovirus promoter. Ad5-yCD/mutTKSR39rep-mIL12 was propagated in human embryonic kidney (HEK) 293 cells and was free of contaminating wild-type adenovirus based on PCR. The viral particle (vp) to plaque-forming unit ratio of the Ad5-yCD/mutTKSR39rep-mIL12 preparation used in the toxicology study was 18. HEK 293, DU145, TRAMP-C2, and primary human prostate epithelial cells (PCS-440-010) were obtained from the American Type Culture Collection (Manassas, VA). Mouse (C57BL/6) primary prostate epithelial cells (C57-6038) were obtained from Cell Biologics, Inc (Chicago, IL). All cell lines were maintained in medium recommended by the supplier.
Primary human prostate epithelial cells pcs 440 010
Manufactured by American Type Culture Collection
Primary human prostate epithelial cells (PCS-440-010) are frozen, cryopreserved cells derived from normal human prostate tissue. They are suitable for use in cell culture applications.
Automatically generated - may contain errors
2 protocols using primary human prostate epithelial cells pcs 440 010
1
Oncolytic Adenovirus Encoding IL-12 and TK
2
Engineered Adenovirus for Dual Cancer Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ad5-yCD/mutTKSR39rep-mIL12 has been described earlier.13 (link) In brief, it is a 55 kDa E1B-deleted replication-competent adenovirus that contains a yeast cytosine deaminase (yCD)/mutant SR39 herpes simplex virus thymidine kinase (mutTKSR39) fusion gene in the E1 region and a single-chain murine IL-12 gene in the E3 region. Both the yCD/mutTKSR39 fusion gene and IL-12 genes are under the transcriptional control of the human cytomegalovirus promoter. Ad5-yCD/mutTKSR39rep-mIL12 was propagated in human embryonic kidney (HEK) 293 cells and was free of contaminating wild-type adenovirus based on PCR. The viral particle (vp) to plaque-forming unit ratio of the Ad5-yCD/mutTKSR39rep-mIL12 preparation used in the toxicology study was 18. HEK 293, DU145, TRAMP-C2, and primary human prostate epithelial cells (PCS-440-010) were obtained from the American Type Culture Collection (Manassas, VA). Mouse (C57BL/6) primary prostate epithelial cells (C57-6038) were obtained from Cell Biologics, Inc (Chicago, IL). All cell lines were maintained in medium recommended by the supplier.
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