Anti gd2 14 g2a

The following antibodies were used in this study: anti-B7-H3 (FM276, Miltenyi Biotech), anti-GD2 (14.G2a, BD Biosciences), human Ig (polyclonal, Thermo Fisher Scientific), anti-mouse IgG (polyclonal, R&D), anti-CD3 (UCHT1, BioLegend), anti-HisTag (J095G45, BioLegend), anti-CD34 (QBEnd10, R&D), anti-ab-TCR (IP26, BioLegend), anti-CD107a (H4A3, BioLegend), anti-cD25 (BC96, BioLegend), anti-CD69 (FN50, BioLegend), anti-Tim3 (F38-2E2, BioLegend), anti-Lag3 (11C3C65, BioLegend), anti-PD-1 (EH12.1, BD Biosciences), anti-mouse CD45 (30-F11, BioLegend), anti-human CD45 (HI30, BioLegend), Ghost Red 780 (Tonbo Biosciences), Zombie Yellow Viability Dye (BioLegend), propidium iodide (Gibco), Cell Trace Violet (Thermo Fisher Scientific), and Precision Count Beads (BioLegend).

For the immunohistochemistry (IHC) tissue, anti-GD2 14G2a (BD Pharmingen, Cat. 554272, used at 1:400 or 1:1200 depending on lot), anti-GD3 R24 (Abcam, Cat. ab11779, used at 1:400 or 1:200 depending on lot), and anti-human HE4/WFDC2 antibody (R&D systems, MAB6274, used at 1:500) were used. The IHC primary incubation was overnight at 4°C, followed by washing and incubation with secondary reagents for 1–2 h at room temperature. We developed anti-GD2 mAb Clone 19 and anti-GD3 mAb Clone 6 for use in quantitative enzyme linked immunosorbent assay (ELISA) evaluation of serum (characterized in Supplementary Figure S6 and reference (17 (link))). Secondary reagents were as follows. For flow cytometry, anti-mouse IgG conjugated to fluorescein (BD Bioscience, Cat. 554011). For ELISA, anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Sigma, Cat. A0168, used at 1:1,000). For IHC, anti-mouse IgG coupled to horseradish peroxidase (HRP) (Vector Laboratories, ZF0718, used at 1:2,000).