Cells were lysed with lysis buffer and heated at 95° C for 10min before electrophoresis/western blot analysis. The primary anti-MCTP1(PA5-42572, Invitrogen) antibodies and anti-GAPDH (60004-1-lg, Proteintech) antibodies were purchased from Proteintech and were recognized with anti-rabbit IgG peroxidase-conjugated antibody (30000-0-AP, Proteintech), followed by an enhanced chemiluminescence reaction (Thermo). Relative levels of proteins were quantified using densitometry with a Gel-Pro Analyzer (Media). The target bands over the GAPDH band were densitometrically quantified, as indicated under each band. All the full-length unprocessed gels of immunoblots were provided in Supplementary Figure 1 of Supplementary materials.
Pa5 42572
Manufactured by Thermo Fisher Scientific
The PA5-42572 is a laboratory equipment product from Thermo Fisher Scientific. It is a compact and reliable device designed for laboratory applications. The product's core function is to provide a precise and consistent environment for various laboratory procedures.
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2 protocols using pa5 42572
1
Western Blot Analysis of MCTP1 Protein
2
Western Blot Analysis of MCTP1
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Cells were lysed with lysis buffer and heated at 95°C for 10min before electrophoresis/western blot analysis. The primary anti-MCTP1 (PA5-42572, Invitrogen) antibodies and anti-GAPDH (60004-1-lg, Proteintech) antibodies were purchased from Proteintech and were recognized with anti-rabbit IgG peroxidase-conjugated antibody (30000-0-AP, Proteintech), followed by an enhanced chemiluminescence reaction (Thermo). Relative levels of proteins were quanti ed using densitometry with a Gel-Pro Analyzer (Media). The target bands over the GAPDH band were densitometrically quanti ed, as indicated under each band.
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