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Ab7613

Manufactured by Abcam
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Ab7613 is a mouse monoclonal antibody that recognizes the PCNA (Proliferating Cell Nuclear Antigen) protein. PCNA is a nuclear protein involved in DNA replication and cell cycle progression.

Automatically generated - may contain errors

Lab products found in correlation

Ab8229, by Abcam (2 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Ab18180, by Abcam (1 mentions) Supersignal chemiluminescence system, by Thermo Fisher Scientific (1 mentions) Chemilmager 4440, by Biozym (1 mentions) Nb400 104, by Novus Biologicals (1 mentions) Ab6885, by Abcam (1 mentions) Ab6463, by Abcam (1 mentions) Pdvf membrane, by Bio-Rad (1 mentions) Bis tris gel, by Thermo Fisher Scientific (1 mentions) Supersignal west femto substrate, by Thermo Fisher Scientific (1 mentions) Chemidoc mp, by Bio-Rad (1 mentions) Sc 52746, by Santa Cruz Biotechnology (1 mentions) Bradford assay, by Bio-Rad (1 mentions)

5 protocols using ab7613

1

Western Blot Protein Detection

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Proteins were isolated and equal amounts were separated by PAGE and transferred to nitrocellulose membranes (Sigma). After blocking, membranes were incubated with primary antibodies (anti-ABCA1, ab18180; anti-β-actin, ab8229; anti-apoA-I, ab7613 [all from Abcam, Cambridge, UK]; and anti-SRBI, NB400-104 [Novus]) at 4°C over night. Membranes were then incubated with the appropriate HRP-coupled secondary antibodies followed by detection using the Super Signal chemiluminescence system (Thermo Scientific, Rockford, IL) and a Chemilmager 4440 (Biozym, Oldendorf, Germany).
Röhrl C., Eigner K., Winter K., Korbelius M., Obrowsky S., Kratky D., Kovacs W.J, & Stangl H. (2014). Endoplasmic reticulum stress impairs cholesterol efflux and synthesis in hepatic cells. Journal of Lipid Research, 55(1), 94-103.
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2

HDL3 Modification by Iron

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In order to identify the modification of HDL3 by iron, ferrous ion (final 60, 120 µM) was treated to purified HDL3 (1 mg of protein), followed by incubation for the designated times at 37 °C in the presence of 5% CO2. After 96 h of incubation, about 2 µg of protein was loaded and electrophoresed on 15% SDS-PAGE gels and detected using anti-human full-length apoA-I goat antibody (ab7613; Abcam, Cambridge, UK) and donkey anti-goat IgG-HRP (ab6885, Abcam, Cambridge, UK) as secondary antibody (1:4000 diluted).
Kim S.H., Yadav D., Kim S.J., Kim J.R, & Cho K.H. (2017). High Consumption of Iron Exacerbates Hyperlipidemia, Atherosclerosis, and Female Sterility in Zebrafish via Acceleration of Glycation and Degradation of Serum Lipoproteins. Nutrients, 9(7), 690.
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3

Immunohistochemical Analysis of Arterial Lipids

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Samples of ILT and wall obtained from AAA and healthy arterial wall were embedded in paraffin, and 4 μm cross-sections were cut. Immunohistochemistry was performed with antibodies against APOA1 (ab7613, abcam) and the lipid peroxidation marker MDA (ab6463, abcam), as described [35 (link)].
Martínez-López D., Camafeita E., Cedó L., Roldan-Montero R., Jorge I., García-Marqués F., Gómez-Serrano M., Bonzon-Kulichenko E., Blanco-Vaca F., Blanco-Colio L.M., Michel J.B., Escola-Gil J.C., Vázquez J, & Martin-Ventura J.L. (2019). APOA1 oxidation is associated to dysfunctional high-density lipoproteins in human abdominal aortic aneurysm. EBioMedicine, 43, 43-53.
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4

Western Blot Analysis of Lipid Markers

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Selected fractions and media were diluted in reducing sample buffer and denatured at 95 °C for 10 minutes after which they were electrophoresed on an 4–12% Bis‐Tris gel (Invitrogen, Breda, the Netherlands) and blotted onto a PDVF membrane (Bio‐Rad Laboratories, Hercules, CA). Membranes were blocked in 3% skim milk powder in tris‐buffered saline (TBS) containing 0.1% Tween−20 for 1 hour at room temperature and incubated overnight at 4°C with antibodies against human ANGPTL3 (BAF3829, R&D Systems), apolipoprotein A1 (ab7613; Abcam, Cambridge, UK), apolipoprotein E (18–272‐197 662; GenWay Biotech, San Diego, CA), or LPL (AF7197; R&D Systems) in 3% skim milk powder in TBS containing 0.1% Tween‐20. Next, membranes were washed and incubated for 1 hour at room temperature with a horseradish peroxidase‐conjugated goat antirabbit or rabbit antigoat secondary antibody (Dako, Santa Clara, CA) in 3% skim milk powder in TBS containing 0.1% Tween‐20. After washing with TBS containing 0.1% Tween‐20, bands were visualized with the SuperSignal West Femto Substrate (Thermo Fisher) on a ChemiDoc MP (Bio‐Rad).
Kraaijenhof J.M., Tromp T.R., Nurmohamed N.S., Reeskamp L.F., Langenkamp M., Levels J.H., Boekholdt S.M., Wareham N.J., Hoekstra M., Stroes E.S., Hovingh G.K, & Grefhorst A. (2023). ANGPTL3 (Angiopoietin‐Like 3) Preferentially Resides on High‐Density Lipoprotein in the Human Circulation, Affecting Its Activity. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(21), e030476.
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5

Analyzing Apolipoprotein A-I and TNF-α

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After 48 hr incubation, the harvested cell was lysed by treatment of RIPA buffer (Radioimmunoprecipitation assay buffer, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris (pH 8.0)). Cell lysates were analyzed by western blot analysis using antihuman apoA-I antibody (Ab7613; Abcam, Cambridge, UK) and GAPDH (Ab8229, Abcam), antitumor necrosis factor (TNF)-α (SC52746, Santa Cruz, CA, USA). Protein content from each lysate was measured using Bradford assay (Bio-Rad, Hercules, CA, USA) before loading equal amounts of protein (25 μg/lane) into 13% SDS-PAGE gels.
Yadav D., Kim S.J., Bae M.A., Kim J.R, & Cho K.H. (2018). The Ability of Different Ketohexoses to Alter Apo-A-I Structure and Function In Vitro and to Induce Hepatosteatosis, Oxidative Stress, and Impaired Plasma Lipid Profile in Hyperlipidemic Zebrafish. Oxidative Medicine and Cellular Longevity, 2018, 3124364.
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