2800m control dna standards

Genomic DNA was extracted from the 40 male whole blood samples using the Invitrogen^® PureLink™ Genomic DNA Mini kit (Life Technologies Inc.). A total of 20 μl of each DNA stock solution (numbered 1–40) was diluted with nuclease-free water (Ambion, Carlsbad, CA, USA) to a final volume of 200 μl. The stock solutions of Promega^® 9948 Male DNA and 2800M Control DNA standards (Promega Corp., Madison, WI, USA; concentration of 10 ng/μl; volume, 25 μl) were diluted with Ambion^® nuclease-free water, to a final volume of 250 μl.

DNA was extracted from 40 whole blood samples using an Invitrogen^® PureLink™ Genomic DNA Mini kit (Life Technologies Inc.). Aliquots (20 µl) of the 40 DNA extracts (nos. 1-40) were diluted by adding nine volumes (180 µl) of Ambion^® Nuclease-Free Water (Life Technologies Inc.) to obtain 10-fold dilutions of the DNA solutions (final volume, 200 µl). The Promega^® stock solutions 9948 Male DNA and 2800M control DNA standards (10 ng/µl; 25 µl; Promega, Corp., Madison, WI, USA) were added with nine volumes (225 µl) of Ambion^® Nuclease-Free Water, to obtain 10-fold dilutions of the standard samples (final volume, 250 µl).