Mitoros kit

Manufactured by AAT Bioquest

Sourced in China

The MitoROS kit is a laboratory product designed to measure mitochondrial reactive oxygen species (ROS) production. It provides a fluorometric assay for the detection and quantification of ROS levels in live cells.

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Lab products found in correlation

Jc 1 kit, by Keygen Biotech (3 mentions) Mptp kit, by BestBio (2 mentions) Annexin 5 fitc pi kit, by Keygen Biotech (1 mentions)

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MitoROS (5 citations)

4 protocols using mitoros kit

Radiation-Induced Mitochondrial Dynamics

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MFC cells were cultured in 6-well plates before incubated with MON@PG (50 μM), meanwhile the plates were either exposed to radiation or not. After 24 h, following the manufacturer’s protocols, the treated cells were collected and tested by a MMP assay kits with JC-1 (Beyotime Biotech, China) and a mitoROS kit (AAT Bioquest, China).

Wang H., Niu H., Luo X., Zhu N., Xiang J., He Y., Chen Z., Li G, & Hu Y. (2023). Radiosensitizing effects of pyrogallol-loaded mesoporous or-ganosilica nanoparticles on gastric cancer by amplified ferroptosis. Frontiers in Bioengineering and Biotechnology, 11, 1171450.

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Mitochondrial Function Assays in CT26 Cells

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After pre‐treating CT26 cells with HAP, HC, HL, or HCL for 24 h, the cells were collected, and mitochondrial functions were assessed using a MitoROS Kit (AAT Bioquest, Wuhan, China), a JC‐1 Kit (Keygen), and an mPTP Kit (BestBio, Shanghai, China), according to the manufacturers' instructions.

Xiao Y., Guo G., Wang H., Peng B., Lin Y., Qu G., Li B., Jiang Z., Zhang F., Wu J, & Liang M. (2023). Curcumin/L‐OHP co‐loaded HAP for cGAS‐STING pathway activation to enhance the natural immune response in colorectal cancer. Bioengineering & Translational Medicine, 9(1), e10610.

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Mitochondrial Dysfunction in LPS-Stimulated HK-2 Cells

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LPS-stimulated HK-2 cells were treated with PP and PPR NPs. After incubation, the cells were harvested and analyzed using the JC-1 Kit (KeyGen), MitoROS Kit (AAT Bioquest, Wuhan, China), mPTP (Mitochondrial Permeability Transition Pore) Kit (BestBio, Shanghai, China), and MitoCheck Complex I Activity Assay Kit (I-V) (Aimei, Wuhan, China).

Feng W., Zhu N., Xia Y., Huang Z., Hu J., Guo Z., Li Y., Zhou S., Liu Y, & Liu D. (2024). Melanin-like nanoparticles alleviate ischemia-reperfusion injury in the kidney by scavenging reactive oxygen species and inhibiting ferroptosis. iScience, 27(4), 109504.

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Phototherapy-Enhanced Cytotoxicity of HMON@CuS/Gd

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HGC-27 cells were pretreated with HMON@CuS/Gd, with or without an 808 nm laser at a power density of 0.8 W/cm² for 5 min respectively. 24 h later, the treated cells were collected and incubated with CCK-8 kit for viabilities detection, with Annexin V-FITC/PI Kit (KeyGEN, Nanjing, China) for apoptosis detection and EDU testing kit (Ruibo, Guangzhou, China) for cell proliferation ability detection. Furthermore, in order to evaluate mitochondrial function, the treated cells were also harvested and detected with JC-1 Kit (Keygen), mitoROS kit (AAT Bioquest, Wuhan, China), ROS Kit (Keygen), mPTP kit (BestBio, Shanghai, China) follow the manual instructions.

Guo W., Chen Z., Chen J., Feng X., Yang Y., Huang H., Liang Y., Shen G., Liang Y., Peng C., Li Y., Li G., Huang W., Zhao B, & Hu Y. (2020). Biodegradable hollow mesoporous organosilica nanotheranostics (HMON) for multi-mode imaging and mild photo-therapeutic-induced mitochondrial damage on gastric cancer. Journal of Nanobiotechnology, 18, 99.

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