Basic hei vap ml

Individually, 400 g of C. fenestratum stem, C. tinctorius flower, and Z. officinale rhizomes were extracted with ethanol for three days using the maceration procedure. The filtrate was collected using Whatman No. 1 filter paper and evaporated using a rotary evaporator to obtain a viscous ethanolic extract (Heidolph Basic Hei-VAP ML, Schwabach, Germany). The maceration procedure was then performed twice more. Each herb’s remaining ethanol was evaporated further in a vacuum drying chamber (Binder VD 23, Tuttlingen, Germany) until a stable weight was obtained.

Initially, 400 g of Z. officinale rhizomes underwent individual extraction using a 50:50 mixture of ethanol and ethyl acetate. The pH of the extraction solution was adjusted to fall within the range of 5.0 to 7.0 by adding 1.0 M NaOH or HCl as needed. The extraction process employed the maceration technique and lasted for a period of three days. Subsequently, the resulting liquid was meticulously filtered through Whatman No. 1 filter paper, and the collected filtrate was then subjected to evaporation using a rotary evaporator (Heidolph Basic Hei-VAP ML, Schwabach, Germany), resulting in the formation of a highly viscous extract dissolved in cosolvent. To ensure optimal extraction efficiency, the maceration process was repeated two more times following the same protocol. The residual ethanol present in the herbal material was eliminated by subjecting it to further evaporation in a vacuum drying chamber (Binder VD 23, Tuttlingen, Germany). Evaporation continued until a consistent weight was attained, indicating the complete removal of all remaining cosolvent.