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Phospho erk p44 42

Manufactured by Santa Cruz Biotechnology

Phospho-ERK p44/42 is a lab equipment product used to detect the phosphorylated forms of the extracellular signal-regulated kinase (ERK) p44 and p42 proteins. It is a key component in signaling pathways and plays a role in cellular processes such as proliferation, differentiation, and survival.

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2 protocols using phospho erk p44 42

1

Extensive Immunohistochemistry and Western Blot Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed using the following antibodies: p16 (Ventana, catalog number 9517), p53 (Dako, catalog number GA6166), Cyclin D1 (ThermoFisher, catalog number RM-914-S), CDK4 (Invitrogen, catalog number AHZ0202), EGFR (Ventana, catalog number 790-4347), MDM2 (Invitrogen, catalog number 182403), Rb (BD Biosciences, catalog number 554136), Met (clone c-28), YAP1 (Cell Signaling, catalog number 4912), phospho-ERK p44/42 (clone D13,14.4E).
Antibodies for Western Blots were as follows: NFKBIE (Santa Cruz, catalog number sc-7155); RELA/p65 (Cell Signaling, 8242); p50/p105 (Cell Signaling, p/n 3035); cREL (Cell Signaling, p/n 4727); PARP (Cell Signaling, 9542); HSP60 (Santa Cruz, sc-1722). NFKBIE siRNAs were from Dharmacon (ON-TARGETplus Human NFKBIE siRNA SMARTpool). Cell fractionation was performed using the Subcellular Protein FractionationKit for Cell Culture (Thermo Scientific 78840). The M257 and M375 cell lines were provided by Dr. Antoni Ribas.
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2

Extensive Immunohistochemistry and Western Blot Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed using the following antibodies: p16 (Ventana, catalog number 9517), p53 (Dako, catalog number GA6166), Cyclin D1 (ThermoFisher, catalog number RM-914-S), CDK4 (Invitrogen, catalog number AHZ0202), EGFR (Ventana, catalog number 790-4347), MDM2 (Invitrogen, catalog number 182403), Rb (BD Biosciences, catalog number 554136), Met (clone c-28), YAP1 (Cell Signaling, catalog number 4912), phospho-ERK p44/42 (clone D13,14.4E).
Antibodies for Western Blots were as follows: NFKBIE (Santa Cruz, catalog number sc-7155); RELA/p65 (Cell Signaling, 8242); p50/p105 (Cell Signaling, p/n 3035); cREL (Cell Signaling, p/n 4727); PARP (Cell Signaling, 9542); HSP60 (Santa Cruz, sc-1722). NFKBIE siRNAs were from Dharmacon (ON-TARGETplus Human NFKBIE siRNA SMARTpool). Cell fractionation was performed using the Subcellular Protein FractionationKit for Cell Culture (Thermo Scientific 78840). The M257 and M375 cell lines were provided by Dr. Antoni Ribas.
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