Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti lrp6

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Rabbit anti-LRP6 is a primary antibody that recognizes the LRP6 protein. LRP6 is a receptor that plays a key role in the Wnt signaling pathway. This antibody can be used to detect and study the LRP6 protein in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti p lrp6, by Cell Signaling Technology (2 mentions) Goat anti mouse igg hrp, by Merck Group (2 mentions) Donkey anti rabbit igg hrp, by Merck Group (2 mentions) Bis tris mini gel, by Thermo Fisher Scientific (2 mentions) Bradford assay, by Bio-Rad (2 mentions) Protease phosphatase inhibitor, by Roche (2 mentions) Iblot, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho lrp6, by Cell Signaling Technology (2 mentions) Rabbit anti oct4a, by Cell Signaling Technology (2 mentions) Clarity western ecl, by Bio-Rad (2 mentions) Mouse anti active β catenin, by Merck Group (2 mentions) Complete lysis m, by Roche (2 mentions) Mouse anti β actin, by Merck Group (2 mentions) Rabbit anti β catenin, by Cell Signaling Technology (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Rat anti ha, by Roche (1 mentions) Goat anti rat igg h l hrp, by Thermo Fisher Scientific (1 mentions) Mouse anti β catenin, by BD (1 mentions) Goat anti rabbit igg h l hrp, by Promega (1 mentions) Goat anti mouse igg h l hrp, by Promega (1 mentions)

Close spelling variants of this product

Anti-LRP6 (11 citations) Rabbit anti-phospho-LRP6 (2 citations) Rabbit anti-LRP6 (C5C7) (2 citations) Rabbit anti-LRP6 mAb (2 citations) Rabbit anti-P-LRP6 (2 citations)

6 protocols using rabbit anti lrp6

1

Immunoblotting with Diverse Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunoblotting: Rat anti-HA (Roche, ROAHAHA), Rabbit anti- FLAG (Proteintech, 20543-1-AP), Mouse anti-GAPDH (Developmental Studies Hybridoma Bank, hGAPDH-2G7), Rabbit anti-USP46 (Proteintech, 13502-1-AP), Mouse anti-Tubulin (Developmental Studies Hybridoma Bank, E7), Mouse anti-β-catenin (BD Transduction Laboratory, 610154), Rabbit anti-Axin1 (Cell Signaling Technology, 2087), Rabbit anti-Insulin Receptor β (Cell Signaling Technology, 3025), Rabbit anti-LRP6 (Cell Signaling Technology, 2560), Rabbit anti-WDR20 (Bethyl Laboratories, A301-657A), Rabbit anti-WDR48 (UAF1) (Proteintech, 16503-1-AP), Goat anti-rat IgG H + L-HRP (Thermo, 31470), Goat anti-mouse IgG H + L-HRP (Promega, W4021), Goat anti-rabbit IgG H + L-HRP (Promega, W4011). All primary antibodies were used at 1:1000 dilution except anti-FLAG (1:2000), anti-GAPDH (1:500), and anti-WDR20 (1:2500). All secondary HRP antibodies were used at 1:5000 dilution.
Ng V.H., Spencer Z., Neitzel L.R., Nayak A., Loberg M.A., Shen C., Kassel S.N., Kroh H.K., An Z., Anthony C.C., Bryant J.M., Lawson A., Goldsmith L., Benchabane H., Hansen A.G., Li J., D’Souza S., Lebensohn A.M., Rohatgi R., Weiss W.A., Weiss V.L., Williams C., Hong C.C., Robbins D.J., Ahmed Y, & Lee E. (2023). The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling. Nature Communications, 14, 6173.
+ Open protocol
+ Expand
2

Western Blot Analysis of Wnt Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction was performed with RIPA buffer (NEB) in the presence of protease and phosphatase inhibitors (Sigma) and quantified using the Bio-Rad DC protein assay (Bio-Rad). Total protein lysates (10–15 ug) were resolved in Bolt bistris plus 4–12% gels (Life technologies). PVDF membranes were blocked for 2 h in 5% (w/v) BSA TBST buffer and the following primary antibodies diluted in blocking buffer were used: Mouse anti-GAPDH (1/5000, Abcam), mouse anti-B-catenin (sc7963, 1/1000, Santa Cruz Biotechnology), rabbit anti-P-Ser552 B-catenin (1/500, Cell signaling), rabbit anti-P-Ser675 B-catenin (1/500, Cell signaling), rabbit anti-LRP6 (1/500, Cell signaling), rabbit anti-P-LRP6 (1/500, Cell signaling). Incubation was performed overnight at 4C and primary antibodies were detected with anti-rabbit and anti-mouse HRP antibodies (Abcam) using the Luminata Crescendo Western HRP substrate (Millipore). Protein samples from 3 independent differentiations were analysed except the XAV treatment analysis where 2 rounds of differentiation were performed.
Cardo L.F., de la Fuente D.C, & Li M. (2023). Impaired neurogenesis and neural progenitor fate choice in a human stem cell model of SETBP1 disorder. Molecular Autism, 14, 8.
+ Open protocol
+ Expand
3

Western Blot Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates from EBs were isolated in RIPA lysis buffer as previously described [8] (link). Protein lysates were separated on 8–10% SDS-PAGE gels as previously described (). Antibodies used included rabbit anti-pSmad1, rabbit anti-Smad1/5, rabbit anti-pSmad2, rabbit anti-Smad2/3, rabbit anti-pErk, rabbit anti-Erk, rabbit anti-p-p38, rabbit anti-p38, rabbit anti-Lrp6, rabbit anti-pLrp6 (all Cell Signaling Technology, USA), mouse anti-beta-catenin (Santa Cruz Biotechnology, USA), cardiac myosin heavy chain (Abcam, USA), anti-CS-E antibody GD3G7 [54] (link), [55] (link), [56] (link), and mouse anti-alpha-tubulin (Santa Cruz Biotechnology, USA).
Prinz R.D., Willis C.M., van Kuppevelt T.H, & Klüppel M. (2014). Biphasic Role of Chondroitin Sulfate in Cardiac Differentiation of Embryonic Stem Cells through Inhibition of Wnt/β-Catenin Signaling. PLoS ONE, 9(3), e92381.
+ Open protocol
+ Expand
4

Immunoblotting and Immunofluorescence Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Immunoblotting: mouse anti-α-Tubulin (Sigma Aldrich, T5168, 1:10,000); mouse anti-actin (MP Biomedicals (CLONE C4), 08691002, 1:10,000); rabbit anti-LRP6 (Cell Signaling (C5C7), #2560, 1:1000); rabbit anti-Phospho-LRP6 (Ser1490) (Cell Signaling, #2568, 1:500). All goat secondary antibodies were conjugated with either Alexa Fluorphores (Life Technologies, A-21109, 1:8000) or IRDyes (LI-COR, P/N 926–32210, 1:8000). For immunofluorescence: rabbit anti-Ki67 (Abcam, 15580, 1:100), rabbit anti-Lysozyme (Dako, A0099, 1:500), mouse anti-ChgA (Santa Cruz (C-12), sc-393941, 1:200). As secondary antibodies goat anti-rabbit IgG-488 (Life Technologies, A-21441, 1:300) and goat anti-mouse-IgG-568 (Life Technologies, A-11004, 1:300) were used. Hoechst 33342 (Sigma, B-2261) was used at 1:500 and Phalloidin (Ph647) (Cell Signaling, #8940) at 1:50.
Fenderico N., van Scherpenzeel R.C., Goldflam M., Proverbio D., Jordens I., Kralj T., Stryeck S., Bass T.Z., Hermans G., Ullman C., Aastrup T., Gros P, & Maurice M.M. (2019). Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells. Nature Communications, 10, 365.
+ Open protocol
+ Expand
5

Western Blot Analysis of LRP6, OCT4A, and β-Catenin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was isolated with cOmplete Lysis-M plus protease/phosphatase inhibitors (Roche) and quantitated by Bradford assay (BioRad). 50 μg protein was separated in 4–12% Bis-Tris mini-gels (Life Technologies), transferred to nitrocellulose membranes via iBlot (Life Technologies), and blocked in 5% BSA diluted in 1x TBS-0.1% Tween (TBS-T) for 1 h while rocking. The following primary antibodies diluted in 5% BSA were incubated overnight at 4 °C: rabbit anti- LRP6 (1:2,000; Cell Signaling; C47E12), rabbit anti-phospho-LRP6 (1:2,000; Cell Signaling; S1490), rabbit anti-OCT4A (1:2,000; Cell Signaling; C30A3), rabbit anti-β-catenin (1:2,000; Cell Signaling; D10A8), mouse anti-active β-catenin (1:2,000; Millipore; 05-665), and mouse anti-β-actin (1:20,000; Sigma; A3853). Membranes were rinsed in TBS-T, followed by incubation with HRP-goat anti-mouse IgG (1:20,000; Sigma-Aldrich; SAB3700993) and HRP-donkey anti-rabbit IgG (1:20,000; Sigma-Aldrich; SAB3700978) for 1 h while rocking. Membranes were rinsed in TBS-T, detected with Clarity Western ECL (BioRad), and exposed in a ChemiDoc XRS+ system (BioRad).
Francis K.R., Ton A.N., Xin Y., O’Halloran P.E., Wassif C.A., Malik N., Williams I.M., Cluzeau C.V., Trivedi N.S., Pavan W.J., Cho W., Westphal H, & Porter F.D. (2016). Modeling Smith-Lemli-Opitz syndrome with iPS cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes. Nature medicine, 22(4), 388-396.
+ Open protocol
+ Expand
6

Western Blot Analysis of LRP6, OCT4A, and β-Catenin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was isolated with cOmplete Lysis-M plus protease/phosphatase inhibitors (Roche) and quantitated by Bradford assay (BioRad). 50 μg protein was separated in 4–12% Bis-Tris mini-gels (Life Technologies), transferred to nitrocellulose membranes via iBlot (Life Technologies), and blocked in 5% BSA diluted in 1x TBS-0.1% Tween (TBS-T) for 1 h while rocking. The following primary antibodies diluted in 5% BSA were incubated overnight at 4 °C: rabbit anti- LRP6 (1:2,000; Cell Signaling; C47E12), rabbit anti-phospho-LRP6 (1:2,000; Cell Signaling; S1490), rabbit anti-OCT4A (1:2,000; Cell Signaling; C30A3), rabbit anti-β-catenin (1:2,000; Cell Signaling; D10A8), mouse anti-active β-catenin (1:2,000; Millipore; 05-665), and mouse anti-β-actin (1:20,000; Sigma; A3853). Membranes were rinsed in TBS-T, followed by incubation with HRP-goat anti-mouse IgG (1:20,000; Sigma-Aldrich; SAB3700993) and HRP-donkey anti-rabbit IgG (1:20,000; Sigma-Aldrich; SAB3700978) for 1 h while rocking. Membranes were rinsed in TBS-T, detected with Clarity Western ECL (BioRad), and exposed in a ChemiDoc XRS+ system (BioRad).
Francis K.R., Ton A.N., Xin Y., O’Halloran P.E., Wassif C.A., Malik N., Williams I.M., Cluzeau C.V., Trivedi N.S., Pavan W.J., Cho W., Westphal H, & Porter F.D. (2016). Modeling Smith-Lemli-Opitz syndrome with iPS cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes. Nature medicine, 22(4), 388-396.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!