Anti cd11b alexa fluor 700 clone m1 70

Manufactured by BD

Sourced in United States

Anti-CD11b Alexa Fluor 700 (clone M1/70) is a fluorescently-labeled antibody that binds to the CD11b antigen. CD11b is a cell surface glycoprotein that is expressed on the surfaces of various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used for the identification and characterization of these cell types in flow cytometry applications.

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Lab products found in correlation

Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti cd44 pe cy7 clone im7, by BioLegend (1 mentions) Anti ifnγ alexafluor 700, by BD (1 mentions) Anti cd3 pacific blue clone 17a2, by BioLegend (1 mentions) Anti foxp3 alexa fluor 488 clone mf23, by BD (1 mentions) Il 2 fitc clone jes6 5h4, by BD (1 mentions) Anti cd4 percp clone rm4 5, by BD (1 mentions) Tnf α pe cy7, by BD (1 mentions) Anti cd8 pe, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions)

2 protocols using anti cd11b alexa fluor 700 clone m1 70

Comprehensive Immune Cell Profiling

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The following Abs were used for cell surface staining: anti-CD3 Pacific Blue (clone 17A2), anti-CD8 PE Texas Red (clone 53.6.7), anti-CD44 PE-Cy7 (clone IM7), anti-B220 PE Texas Red (clone RA3-6B2), anti-NK1.1 FITC (clone PK136), anti-CD69 Alexa Fluor 647 (clone H1-2F3), anti-Ly6C APC-Cy7 (AL-21), anti-Ly6G PE-Cy7 (clone 1A8) (Biolegend, San Diego, CA, USA), anti-CD4 APC e-Fluor 780 (clone GK1.5), anti-CD62L PE-Cy5 (clone MEL-14) (eBiosciences, San Diego, CA, USA), anti-CD45RB PE (clone 16A), anti-CD3 Alexa Fluor 647 (clone 17A2), anti-CD4 PerCP (clone RM4-5), anti-CD8 PE (clone 53-6.7), anti-CD45 PE-Cy5 (clone 30-F11), anti-CD45 PE (clone 30-F11), anti-CD11c FITC (clone HL3) and anti-CD11b Alexa Fluor 700 (clone M1/70) (BD Biosciences, San José, CA, USA). The abs for intracellular staining included anti-FoxP3 Alexa Fluor 488 (clone MF23), anti-IFNγ Alexa Fluor 700 (clone XMG1.2), TNFα PE-Cy7 (clone MP6-XT22), IL-2 FITC (clone JES6-5H4) (BD Biosciences) and anti-CTLA-4 PE (clone UC10-4F10-11) (Biolegend). A LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen Molecular Probes, Eugene, OR) was used for dead cell exclusion.

Lasso P., Gomez-Cadena A., Urueña C., Donda A., Martinez-Usatorre A., Barreto A., Romero P, & Fiorentino S. (2018). Prophylactic vs. Therapeutic Treatment With P2Et Polyphenol-Rich Extract Has Opposite Effects on Tumor Growth. Frontiers in Oncology, 8, 356.

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Comprehensive Analysis of Tumor-Infiltrating Myeloid Cells

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CEA_526–533 and HIV-GAG tetramer staining were performed as previously described (1 (link)). To analyze TIMs, 21-day-old MC38-CEA tumors were harvested and enzymatically digested to obtain a single-cell suspension (1 (link)). Anti-CD11b Alexa Fluor 700 clone M1/70 and anti-Gr1 APC-Cy7 clone RB6-8C5 were purchased from BD Biosciences (Franklin Lakes, NJ). Anti-CXCL9 Alexa Fluor 647 clone MIG-2F5.5, anti-CD105 PerCp-Cy5.5 clone MJ7/18, and anti-CD31 Pacific Blue clone 390 were purchased from BioLegend (San Diego, CA). Anti-CD45 eFluor 605NC clone 30-F11 and anti-FAS-L PerCP-eFluor 710 clone MFL3 were purchased from eBioscience (San Diego, CA). At least 3 × 10⁵ live cells were acquired with an LSR-II flow cytometer (BD Biosciences) and data were analyzed with FlowJo software for PC (TreeStar Inc., Ashland, OR).

Farsaci B., Donahue R.N., Coplin M.A., Grenga I., Lepone L.M., Molinolo A.A, & Hodge J.W. (2014). Immune Consequences of Decreasing Tumor Vasculature with Antiangiogenic Tyrosine Kinase Inhibitors in Combination with Therapeutic Vaccines. Cancer immunology research, 2(11), 1090-1102.

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