Y1v1hoptum fm7c

Flies were raised on standard media. Crosses were raised at 25°C unless otherwise noted. 10xstat92E-GFP (BL#26197) [22 (link)], UAS-eGFP (BL#5430), UAS-eGFP (BL#5431), hsflp¹²²;;Ubi-RFP,FRT79 (made from BL#34498), y¹v¹hop^Tum/FM7c (BL#8492; referred to as hop^Tum-l), act5c-GAL4/CyO (BL#4414), UAS-MYR-RFP/CyO (BL#7118), hml-GAL4 (BL#30139) and stat⁰⁶³⁴⁶ (BL# 11681) were obtained from Bloomington Drosophila Stock Center, Bloomington, IN. UAS-hipk^RNAi (VDRC ID#108254, [23 (link)]) was obtained from Vienna Drosophila Resource Center, Vienna, Austria. Also used were dpp-GAL4/TM6B [24 (link)], os,y (a gift from Norbert Perrimon), UAS-Stat92E-GFP/Cyo and UAS-Stat92E-MYC/Cyo,wg-lacZ (a gift from James Castelli-Gair Hombria, [25 (link),26 (link)], PD-lacZ (a gift from Henry Sun; referred to as upd¹-lacZ hereon after, Tsai and Sun, 2004 [27 (link)]), ywhsflp,tub-GAL4,UAS-GFP,6X MYC-NLS; UAS-y+;tub-GAL80,FRT2A/TM6B (a gift from Gary Struhl), ywhsflp¹²²;sp/Cyo;TM2/TM6B, UAS-HA-hipk^1M, UAS-HA-hipk^3M, hipk⁴,FRT79/TM6B [28 (link)], UAS-HA-hipk^WT-attP40, UAS-MYR-HA-hipk-attP40, UAS-NLS-HA-hipk-attP40 (made in this study). act5c-GAL4/CyO and UAS-MYR-RFP/CyO were recombined to generate act5c-GAL4, UAS-MYR-RFP/CyO. hipk⁴, FRT79/TM6B and 10xstat92E-GFP/TM6B were recombined to generate hipk⁴, FRT79,10xstat92E-GFP/TM6B.

The CG9989 (sid) RNAi line was obtained from the Fly Stocks of National Institute of Genetics (NIG-FLY, Japan). The W¹¹¹⁸, y¹v¹ hop^Tum/FM7c, and W¹¹¹⁸;Df(3R)Exel6209,P{XP-U}Exel6209/TM6B,Tb¹ were obtained from Bloomington Drosophila Stock Center at Indiana University. The ubiquitin-GAL4 line was kindly provided by Dr. Utpal Banerjee at University of California, Los Angeles and the UAS-dicer line was obtained from Dr. Kyung-An Han at The University of Texas at El Paso. The UAS-sid line was generated by BestGene (Chino Hills, CA). Additionally, sid knock down and ubiquitous over expression lines were generated using standard genetic methods by crossing of flies carrying the UAS-sid RNAi, UAS-sid, W⁻/Ubiquitin-GAL4;Df(3R)Exel6209,P{XP-U}Exel6209/UAS-sid RNAi, Ubiquitin-GAL4;UAS-sid RNAi, Ubiquitin-GAL4;UAS-sid and Ubiquitin-GAL4;UAS-dicer;UAS-sid RNAi. All flies were maintained in standard corn meal/agar medium (110 g sucrose, 52 g cornmeal, 27.4 g yeast, 7 g agar, 2.4 g methyl p-hydroxybenzoate/8.5 ml ethanol, and 1.5 g propionic acid/L) at 25°C except that the temperature was increased to 29°C during septic infection. Flies were kept in humidified incubators programmed for 12 hour day/night periods.