Sputum specimens were first decontaminated and digested using the standard N-acetyl L-cysteine/4% NaOH solution and concentrated by centrifugation (4,500 rpm). The pellets were used for smear microscopy staining using the auramine–rhodamine method. Both liquid (Mycobacteria Growth Incubator Tube [BBL™ MGIT™ Becton Dickinson, Sparks, MD]) and solid (Middlebrook 7H11 agar and selective 7H11 agar, Lenexa, KS) media were inoculated. Speciation of positive acid-fast bacilli cultures was performed with nucleic acid probes (AccuProbe® GenProbe, San Diego, CA). Mycobacterium tuberculosis complex (MTBc) isolates were spoligotyped using a commercially available kit (Isogen Life Science, De Meern, The Netherlands) to differentiate MTB and MAF species and subspecies.
Bbl mgit
Manufactured by BD
Sourced in United States
The BBL™ MGIT is a laboratory equipment used for the rapid detection and identification of mycobacteria in clinical samples. It utilizes a fluorescent sensor to monitor the growth of mycobacteria in a liquid culture medium, providing automated and continuous monitoring of sample growth.
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Lab products found in correlation
4 protocols using bbl mgit
1
Mycobacterial Identification Protocol
2
Mycobacterium Isolation and Identification
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In the UCRC laboratory, initial strain isolation is done in both liquid (manual reading of Mycobacterium Growth Incubator Tubes (BBL™ MGIT™ Becton Dickinson, Sparks MD, USA)), and solid (Middlebrook 7H11 Agar and Selective 7H11 Agar) media, following standard protocols (10, 12). Speciation of positive mycobacterial cultures was based on Acid-Fast Bacilli-positivity on smear microscopy and colony morphologies on solid medium, with was confirmation by Capilia TB Test (TAUNS Laboratories, Numazu, Japan), or by nucleic acid probes (AccuProbe® GenProbe, San Diego, CA, USA).
3
Pulmonary TB Diagnostic Evaluation
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Symptoms of cough greater than two weeks, productive cough, hemoptysis, chest pain, fever, sweats, weight loss or weakness were solicited. All participants, with the exclusion of pregnant women, underwent a chest X-ray which was interpreted by a study radiologist or pulmonologist who was blinded to the symptom questionnaire. Those with any symptoms or suspicious X-ray findings underwent further testing, including sputum collection. Sputum samples were analyzed with fluorescence microscopy using Auramine O staining procedure, and nucleic acid amplification testing (GeneXpert, Cepheid) followed by mycobacterial culture in liquid media (BBL™ MGIT™, Becton, Dickinson and Company) and solid Löwenstein-Jensen media slants (BBL™ Lowenstein-Jensen Medium, Becton, Dickinson and Company). Further workup of computed tomography, bronchoscopy, and/or fine-needle aspiration were performed as indicated by a study pulmonologist.
Active TB cases were defined as positive laboratory testing (smear, culture, or nucleic acid amplification test) in a patient with suggestive symptoms and/or imaging.
Active TB cases were defined as positive laboratory testing (smear, culture, or nucleic acid amplification test) in a patient with suggestive symptoms and/or imaging.
4
Rapid Diagnosis of HIV-TB Coinfection
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All the laboratory work was performed in the BSL-3 facility within the Human Immunodeficiency Virus and Tuberculosis (HIV-TB) Research and Training Center (SEREFO Laboratories) at the University of Sciences, Techniques and Technologies of Bamako (USTTB).
Tissue specimens were washed with sterile saline solution (Remel Inc., Lenexa, KS 66215, USA). Specimens were then cut into small pieces using the Tissue Grinder (Precision Disposable Tissue Grinder, Covidien, Mansfield, MD, USA). Thereafter, specimens were digested and decontaminated using the standard N-Acetyl-L-Cysteine/4 % NaOH solution (Alpha Tec System. Inc., Vancouver, USA), concentrated by high speed centrifugation (4500 rpm or 3000 g, Eppendorf centrifuge) and inoculated on both liquid (Mycobacterium Growth Incubator Tube, BBL™ MGIT™ Becton Dickinson, Sparks MD, USA), and solid (Middlebrook 7H11 Agar and Selective 7H11 Agar) media. Simultaneously, an aliquot of concentrated specimen was prepared for Auramine-Rhodamine staining (BBL™ Becton Dickinson, Sparks MD, USA) for microscopic examination (Olympus, Olympus Corporation, Tokyo, Japan).
Tissue specimens were washed with sterile saline solution (Remel Inc., Lenexa, KS 66215, USA). Specimens were then cut into small pieces using the Tissue Grinder (Precision Disposable Tissue Grinder, Covidien, Mansfield, MD, USA). Thereafter, specimens were digested and decontaminated using the standard N-Acetyl-L-Cysteine/4 % NaOH solution (Alpha Tec System. Inc., Vancouver, USA), concentrated by high speed centrifugation (4500 rpm or 3000 g, Eppendorf centrifuge) and inoculated on both liquid (Mycobacterium Growth Incubator Tube, BBL™ MGIT™ Becton Dickinson, Sparks MD, USA), and solid (Middlebrook 7H11 Agar and Selective 7H11 Agar) media. Simultaneously, an aliquot of concentrated specimen was prepared for Auramine-Rhodamine staining (BBL™ Becton Dickinson, Sparks MD, USA) for microscopic examination (Olympus, Olympus Corporation, Tokyo, Japan).
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