Sealsafe

All animal experiments were carried out in accordance with the Guidelines for Animal Experiments of the National Institute of Infectious Diseases (NIID). Experimental protocols were reviewed and approved by Institutional Animal Care and Use Committee of the Temasek Life Sciences Laboratory, National University of Singapore, Singapore. (IACUC approval number TLL-14-015). Mice were housed in individually ventilated cages (Tecniplast Sealsafe), provided with water and standard chow, and monitored daily for health and clinical signs. More than 25% body weight loss was used as the criterion for early euthanasia. The animals were euthanized by CO₂ inhalation for five minutes.

All animal experimental protocols were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of the Temasek Life Sciences Laboratory, National University of Singapore, Singapore. (IACUC approval number TLL-14-020). All animal experiments were carried out in animal BSL3 containment facility in compliance with CDC/NIH and WHO recommendations. Mice were housed in individually ventilated cages (Tecniplast Sealsafe) provisioned with water and standard food, and monitored daily for health condition. More than 25% body weight loss was used as criterion for early euthanasia. The animals were euthanized by CO₂ inhalation for five minutes.

Female SCID and normal Swiss mice were housed at the SOL-GEL laboratory at the NCSR “Demokritos” and SCID mice were xenografted at two weeks of age, subcutaneously just above the right flank, with U87MG cells that were previously grown in DMEM, as previously described [37 (link)]. The tumor volume, mice weights, and survival rates were calculated in different time intervals. Mice were housed in groups of three per cage under positive pressure in polysulfone type IIL individual ventilated cages (Sealsafe, Tecniplast, Buguggiate, Italy) and had ad libitum access to water and food. Room temperature and relative humidity were 24 ± 2 °C and 55 ± 10% respectively. All animals in the facility were screened regularly according to the Federation of European Laboratory Animal Science Associations’ recommendations and were found to be free of the respective pathogens. Animals were sacrificed under deep ether anesthesia and the brain tissues were rapidly extracted, placed in a polypropylene tube, immediately snap-frozen in liquid nitrogen, and stored at −80 °C.