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Primary antibodies for γh2ax

Manufactured by Santa Cruz Biotechnology
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Primary antibodies for γH2AX are used to detect the phosphorylated form of the histone H2AX protein, which is a marker for DNA double-strand breaks.

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Lab products found in correlation

Guava easycyte, by BD (1 mentions) Alexa fluor 488, by Cell Signaling Technology (1 mentions) 8 ohdg fitc antibody, by Santa Cruz Biotechnology (1 mentions)

2 protocols using primary antibodies for γh2ax

1

DNA Damage Assessment by Flow Cytometry

Cited 1 time
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After cell harvesting and fixation, γH2AX primary antibody (Santa Cruz Biotechnology; Santa Cruz, CA, USA) (1:500 dilution), secondary antibody conjugated Alexa Fluor®488 (Cell Signaling Technology) (1:10000 dilution), and 7AAD (5 μg/mL) were chosen for flow cytometry reaction [32 (link)] and analyzed by a flow cytometer (Guava easyCyte) applying FlowJo software (Becton-Dickinson). By Western blotting, γH2AX was also probed using primary antibodies for γH2AX (Santa Cruz Biotechnology; Santa Cruz, CA, USA) (diluted 1:1000).
Peng S.Y., Wang Y.Y., Lan T.H., Lin L.C., Yuan S.S., Tang J.Y, & Chang H.W. (2020). Low Dose Combined Treatment with Ultraviolet-C and Withaferin a Enhances Selective Killing of Oral Cancer Cells. Antioxidants, 9(11), 1120.
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2

Quantifying DNA Damage Markers via Flow Cytometry

Cited 1 time
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Levels of γH2AX and 8-OHdG [42 (link)] are proportional to the fluorescent intensities generated by antibody detection to the 75% ethanol fixed cells. Primary antibodies for γH2AX [42 (link)] (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and FITC-8-OHdG antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used to incubate with these fixed cells at 4 °C, 1 h. Notably, secondary antibody (Cell Signaling Technology, Danvers, MA, USA) and 7AAD (5 μg/mL, 30 min) were further applied for γH2AX detection. The Guava easyCyte flow cytometer assessed these intensities. Their counts for (+) regions were classified as γH2AX and 8-OHdG (+) cells.
Shiau J.P., Chuang Y.T., Tang J.Y., Chen S.R., Hou M.F., Jeng J.H., Cheng Y.B, & Chang H.W. (2022). Antiproliferation Effects of Marine-Sponge-Derived Methanol Extract of Theonella swinhoei in Oral Cancer Cells In Vitro. Antioxidants, 11(10), 1982.
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