Rabbit anti jak1

The antibodies used for western were as follows: mouse anti-Gfap (Sigma), rabbit anti-Jak1 (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-Il6st (Santa Cruz, C-20), rabbit anti-Lifr-beta (Santa Cruz, C-19), rabbit anti-Stat1 (BD Biosciences, San Jose, CA), mouse anti-Stat3 (BD Biosciences), mouse anti-phosphotyrosine Stat1 (BD Biosciences), rabbit anti-phosphotyrosine Stat3 (Cell Signaling, Danvers, MA), mouse anti-TuJ1 (Covance, Princeton, NJ) and mouse anti-β-actin (Actb) (Sigma). Secondary goat anti-mouse or anti-rabbit IgG-horseradish antibodies (Calbiochem, EMD Millipore, Billerica, MA) were used, and detection was performed using the ECL plus chemiluminescence (PerkinElmer, Waltham, MA) on X-Omat Blue films (Kodak).

Immunoblotting was done as previously described (15 (link)) employing the following antibodies and dilutions: anti-NS3 antibodies were described in Ref. (48 (link)), rabbit anti-phospho-Tyr701-STAT1 (cat. #9167; 1:1,000), rabbit anti-STAT1 (cat. #9172; 1:1,000), rabbit anti-phospho-Tyr690-STAT2 (cat. #88410; 1:1,000), rabbit anti-STAT2 (cat. #72604; 1:1,000) rabbit anti-phospho-Tyr705-Stat3 (cat. #9145; 1:1,000), mouse anti-STAT3 (cat. #9139; 1:1,000), and rabbit anti-SOCS3 (cat. #2923; 1:1,000), (all from cell signaling); mouse anti-phospho-ERK1/2 (Sigma-Aldrich, cat. #M8159; 1:500), rabbit anti-ERK1/2 (Santa-Cruz Biotechnology, cat. #sc-154; 1:10,000), rabbit-anti-JAK1 (Santa-Cruz Biotechnology, cat. #sc-277; 1:400), mouse anti-Actin (MP Biomedicals, cat. #69100; 1:10,000), rabbit anti-GAPDH (Abcam, cat. #ab8245; 1:5,000), mouse anti-GFP (MBL, cat. #M048-3; 1:1,000), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, cat. #115035003; 1:15,000).

The following primary antibodies were used: Mouse anti-FLAG (Sigma, F3165, 1:1,000), Mouse anti-tubulin (Sigma, T9026, 1:10,000), Rabbit anti-STAT1 or 2 (Cell Signaling, 9127, 72604, 1:1,000), Rabbit anti-phospho-STAT1 or 2 (Cell Signaling, 88410, 9167, 1:1,000), Rabbit anti-V5 (Cell Signaling, 13202, 1:1,000), Rabbit anti-JAK1 (Santa-Cruz Biotechnology, sc-277; 1:400), Rabbit anti-phospho-JAK1 (Cell Signaling, 74129, 1:1000), Rabbit anti-lamin (Abcam, 1:1000). The secondary antibodies used were HRP anti-mouse and anti-rabbit (Jackson Immuno Research, 1:5000), Alexa fluor 488 goat anti-rabbit (Invitrogen, A11034, 1:500) or Alexa fluor 555 goat anti-rabbit (Invitrogen, A27039, 1:500).