10m duragard capillary column

The main volatile compounds in the extracts were analysed using GC-q-TOF-MS. The analysis was performed employing a 7890B Agilent system (Agilent Technologies, Santa Clara, CA, USA) coupled to a quadrupole time-of-fight mass spectrometer (q-TOF-MS) 7200 (Agilent Technologies, Santa Clara, CA, USA) equipped with an electronic ionization (EI) source. One microlitle of each extract was injected with a split ratio of 10:1 and a split flow of 8.4 mL min -1 with the injector at a temperature of 250 °C. The separation of compounds was achieved using an Agilent Zorbax DB5-MS + 10 m Duragard Capillary Column (30 m × 250 µm x 0.25 µm). Helium was used as carrier gas at a constant flow rate of 0.8 mL min -1 . The column temperature was maintained at 60 °C for 1 min, then increased at a rate of 10 °C/min to 325 °C, and held at this temperature for 10 min. MS parameters were the following: electron impact ionization at 70 eV, filament source temperature of 250 °C, quadrupole temperature of 150 °C, m/z scan range 50-600 amu at a rate of 5 spectra per second.