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Mircury lna microrna array

Manufactured by Qiagen
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Sourced in Denmark, United States

The MiRCURY LNA microRNA Array is a high-performance microRNA (miRNA) profiling platform developed by Qiagen. It enables comprehensive and accurate analysis of miRNA expression levels across a wide range of species. The array utilizes Locked Nucleic Acid (LNA) technology to provide enhanced specificity and sensitivity for miRNA detection and quantification.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (5 mentions) Genepix pro 6, by Molecular Devices (4 mentions) Mirvana mirna isolation kit, by Thermo Fisher Scientific (4 mentions) Mirneasy mini kit, by Qiagen (4 mentions) Genepix 4000b scanner, by Molecular Devices (3 mentions) Mircury hy3 hy5 power labeling kit, by Qiagen (3 mentions) Mircury array labeling kit, by Qiagen (2 mentions) Rneasy mini kit, by Qiagen (2 mentions) Agilent g2565ba microarray scanner system, by Agilent Technologies (2 mentions) Hs4800 hybridization station, by Tecan (2 mentions) Mircury lna microrna hi power labeling kit, by Qiagen (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Label it mirna labeling kits version 2, by Mirus Bio (2 mentions) Glassarray hybridization cassettes, by Thermo Fisher Scientific (2 mentions) Hiseq 2000, by Illumina (2 mentions) Axon genepix 4000b microarray scanner, by Molecular Devices (2 mentions) Mirneasy kit, by Qiagen (2 mentions) Mircury lna array, by Qiagen (2 mentions) Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Mircury array labelling kit, by Qiagen (1 mentions)

52 protocols using mircury lna microrna array

1

Microarray Analysis of miRNA Expression in Oxaliplatin-Resistant Cells

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Prior to experimentation, HCT116-OxR cells and HT29-OxR cells were cultured 1 week without oxaliplatin. Total RNA from HCT116, HCT116-OxR, HT29 and HT29-OxR cell lines was isolated with Trizol reagent (Invitrogen, Carlsbad, CA) and miRNA fraction was further purified using a mirVana™ miRNA isolation kit (Ambion, Austin, TX). The isolated miRNAs was labeled with Hy3 using the miRCURY™ Array Labelling kit (Exiqon, Vedbaek, Denmark) and hybridized on a miRCURYTM LNA microRNA Array (v 8.0, Exiqon) as described [37 (link)]. Microarray images were acquired using a Genepix 4000B scanner (Axon Instruments, Union City, CA) and processed and analyzed with Genepix Pro 6.0 software (Axon Instruments) and Excel.
Xu K., Chen G., Qiu Y., Yuan Z., Li H., Yuan X., Sun J., Xu J., Liang X, & Yin P. (2017). miR-503-5p confers drug resistance by targeting PUMA in colorectal carcinoma. Oncotarget, 8(13), 21719-21732.
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2

Profiling miRNA Expression under Hypoxia

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Total RNA was extracted from MB-231 and MB-468 cells cultured under normoxic or hypoxic conditions with TRIzol reagent (Invitrogen, Carlsbad, CA), and the miRNA fraction was further purified with the mirVanaTM miRNA isolation kit (Ambion, Austin, TX). A miRCURYTM Array Labeling kit (Exiqon, Vedbaek, Denmark) was used to label the isolated miRNA with Hy3, and samples were hybridized on a miRCURYTM LNA microRNA Array (v 8.0, Exiqon). A Genepix 4000B scanner (Axon Instruments, Union City, CA) was used to acquire microarray images, and Genepix Pro 6.0 software (Axon Instruments) and Excel were used for data processing and analysis.
Li H., Su X., Li J., Liu W., Pan G., Mao A., Liu J., Zhang Q., Rao L., Xie X, & Sheng X. (2022). Hypoxia induces docetaxel resistance in triple-negative breast cancer via the HIF-1α/miR-494/Survivin signaling pathway. Neoplasia (New York, N.Y.), 32, 100821.
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3

Macrophage miRNA Profiling via miRCURY LNA Microarray

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Total RNAs were purified from macrophages with miRNeasy Mini Kit (Qiagen). The miRNA array was performed by Exiqon using miRCURY LNA™ microRNA Array (Exiqon). The data were deposited at Gene Expression Omnibus (GEO) with an accession number GSE55414 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55414).
Xie N., Cui H., Banerjee S., Tan Z., Salomao R., Fu M., Abraham E., Thannickal V.J, & Liu G. (2014). miR-27a regulates inflammatory response of macrophages by targeting IL-10. Journal of immunology (Baltimore, Md. : 1950), 193(1), 327-334.
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4

Comprehensive miRNA Expression Analysis

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MiRNA expression was analysed with specific arrays miRCURY LNA microRNA Array, v.10.0 (Exiqon), following the manufacturer’s recommendations. Then, fluorescent reagents were reconstituted and cDNAs were synthesized and labelled. Following this, samples were heated at 95° C in darkness, hybridized, and washed with the robotic HS 4800 Pro system (Tecan®). Fluorescence was scanned with a GenePix 4000B (Axon Instruments™) microarray scanner. Image processing was accomplished with the Gene Prix Pro (v 6.0). The data for the analysis were generated from a single channel following manufacturer’s instructions. Raw data processing was performed with the ExiMiR package from R 28 , 29 . Quantile normalization was performed using the Robust Microarray Analysis (RMA) algorithm30 (link) from the BioConductor (http://www.bioconductor.org/) tools suite. All expression data were deposited in the GEO database (GEO: GSE71923).
Cañueto J., Cardeñoso-Álvarez E., García-Hernández J.L., Galindo-Villardón P., Vicente-Galindo P., Vicente-Villardón J.L., Alonso-López D., De Las Rivas J., Valero J., Moyano-Sanz E., Fernández-López E., Mao J.H., Castellanos-Martín A., Román-Curto C, & Pérez-Losada J. (2017). miR-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma. The British journal of dermatology, 177(1), 168-178.
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5

Comprehensive Analysis of LUAD Transcriptome

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The public GEO database is a functional genomic database found at http://www.ncbi.nlm.nih.gov/geo. Four datasets (GSE101684, GSE101586, GSE135918, and GSE130779) were downloaded. The circRNA dataset GSE101684 included four LUAD tissues and four adjacent normal lung tissues, and the samples were tested by the GPL21825 074301 Arraystar Human CircRNA microarray V2 platform. The circRNA dataset GSE101586 included four LUAD tissues and four lung tissues, and the samples were tested by the GPL19978 Agilent-069978 Arraystar Human CircRNA microarray V1 platform. The miRNA dataset GSE135918 included five LUAD tissues and five adjacent normal lung tissues, and the samples were tested by the GPL18058 Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed Probe_ID version] platform. The mRNA dataset GSE130779 included eight LUAD tissues and eight adjacent non-tumor tissues; the platform used for the microarray was GPL20115 Agilent-067406 Human CBC lncRNA + mRNA microarray V4.0 (Probe name version).
Wang Z., Pei H., Liang H., Zhang Q., Wei L., Shi D., Chen Y, & Zhang J. (2021). Construction and Analysis of a circRNA-Mediated ceRNA Network in Lung Adenocarcinoma. OncoTargets and therapy, 14, 3659-3669.
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6

miRNA Expression Profiling in A549 Cells

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Total RNAs from A549 and A549 cells with TGF-β1 treatment were isolated with Trizol reagent (Invitrogen, Carlsbad, CA, USA), and miRNA fractions were further purified using a mirVana miRNA isolation kit (Ambion, Austin, TX, USA). The isolated miRNAs from two different cell lines were labeled with Hy3 using the miRCURY Array Labeling kit (Exiqon, Vedbaek, Denmark) and hybridized, respectively, on a miRCURY LNA microRNA Array (v 8.0, Exiqon) as was described.57 (link) Microarray images were acquired using a Genepix 4000B scanner (Axon Instruments, Union City, CA, USA), processed and analyzed using Genepix Pro 6.0 software (Axon Instruments).
Shi Z.M., Wang L., Shen H., Jiang C.F., Ge X., Li D.M., Wen Y.Y., Sun H.R., Pan M.H., Li W., Shu Y.Q., Liu L.Z., Peiper S.C., He J, & Jiang B.H. (2017). Downregulation of miR-218 contributes to epithelial–mesenchymal transition and tumor metastasis in lung cancer by targeting Slug/ZEB2 signaling. Oncogene, 36(18), 2577-2588.
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7

Amygdala Stimulation Induced Temporal Lobe Epilepsy

Cited 1 time
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The expression profile under the accession number GSE49850 (18 (link)) was downloaded from the public functional genomics data repository, Gene Expression Omnibus, which was based on the GPL17566 platform (miRCURY LNA microRNA array; Exiqon, Woburn, MA, USA). A total of 686 miRNAs in rats were analyzed in GSE49850. Data sets consisted of miRNA expression levels at 7, 14, 30 and 90 days subsequent to electrical stimulation of the amygdala, which was used as a model of temporal lobe epilepsy. The sham-operated time-matched control group (N) and the stimulated group (C) were included at each time point with 5 replicates of each.
MENG F., YOU Y., LIU Z., LIU J., DING H, & XU R. (2014). Neuronal calcium signaling pathways are associated with the development of epilepsy. Molecular Medicine Reports, 11(1), 196-202.
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8

Degenerative Disc Disease miRNA Expression

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The GSE63492 miRNA expression dataset [15 (link)] was obtained from the National Center of Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database. This dataset consists of 10 samples, including 5 degenerated nucleus pulposus tissues and 5 normal nucleus pulposus tissues. The microarray data were generated based on the GPL19449 platform [(Exiqon miRCURY LNA microRNA Array, 7th generation REV - hsa, mmu, and rno (miRBase v18.0)]. We obtained degenerative nucleus pulposus samples from IDD patients. Non-degenerative specimens were collected from cadaveric donors. Samples gathered from graded I (Pfirrmann grading) [16 (link)] cadaveric donors were defined as the normal group, whereas patient samples graded IV or V belonged to the IDD group.
Liu J., Li R, & Lyv P. (2023). Identification and Verification of Key MiRNAs Associated with Intervertebral Disc Degeneration. Combinatorial Chemistry & High Throughput Screening, 26(9), 1766-1774.
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9

Integrated Transcriptome Analysis of IDD

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A microarray dataset was retrieved from the public GEO database (www.ncbi.nlm.nih.gov/geo); the accession number of the dataset used is GSE67567, which contains three sub-datasets: GSE67566, circRNA expression profile (platform: GPL19978, Agilent-069978 Arraystar Human CircRNA microarray V1; Agilent Technologies, Inc., Santa Clara, CA, USA) (12 (link),13 (link)); GSE63492, miRNA expression profile [platform: GPL19449, Exiqon miRCURY LNA microRNA Array, 7th generation REV-hsa, mmu & rno (miRBase v18.0); Exiqon; Qiagen, Inc., Valencia, CA, USA] (12 (link),13 (link)); and GSE56081, mRNA-lncRNA expression profile [platform: GPL15314, Arraystar Human LncRNA microarray V2.0 (Agilent_033010 Probe Name version); Agilent Technologies, Inc.] (12 (link),14 (link)). These three sub-datasets included NP samples derived from five normal control individuals and five patients with IDD.
Zhu J., Zhang X., Gao W., Hu H., Wang X, & Hao D. (2019). lncRNA/circRNA-miRNA-mRNA ceRNA network in lumbar intervertebral disc degeneration. Molecular Medicine Reports, 20(4), 3160-3174.
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10

miRNA microarray analysis protocol

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Tissue specimens (vide supra) were analyzed by miRNA microarray using a miRCURY LNA™ microRNA array (7th generation, Exiqon, Denmark), based on miRBase version 18.0. Briefly, RNA was isolated using TRIzol® Reagent (Invitrogen, Carlsbad, U.S.A.) and then purified with an RNeasy mini kit (Qiagen, Hilden, Germany). The quantity and quality of the extracted RNA was determined with the aid of an ND-1000 Nanodrop spectrophotometer (Nanodrop Technologies, U.S.A.) and the veracity of RNA was established by using gel electrophoresis.
Each microarray slide was scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, U.S.A.) and examined using GenePix Pro version 6.0 (Axon) to measure the intensity of each raw image.
Hu M., Wang X., Liu N., Ding K., Zhang G, & Liu X. (2021). Differential expression of miRNAs as biomarkers for predicting the outcomes of diffuse large B-cell lymphoma patients. Bioscience Reports, 41(7), BSR20201551.
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