HPLC grade formic acid was obtained from Merck (Muskegon), and acetonitrile was purchased from Thermo Fisher. Other reagents, unless otherwise specified, including cryo-gel, 0.5% eosin solution, and hematoxylin, were purchased from Sigma-Aldrich. Acyl-CoA thioesterase 2 (15633-1-AP), choline/ethanolamine phosphotransferase 1 (20496-1-AP), and p62 (18420-1-AP) primary antibodies were obtained from Proteintech. LC3 (83506) primary antibody was purchased from Cell Signalling Technology.
Hplc grade formic acid
Manufactured by Merck Group
Sourced in United States, Germany, Italy
HPLC-grade formic acid is a high-purity chemical compound used as a mobile phase additive in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a distinctive odor. HPLC-grade formic acid is designed to meet the strict purity requirements necessary for HPLC analysis, ensuring consistent and reliable chromatographic results.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade acetonitrile, by Merck Group (23 mentions)
Methanol, by Merck Group (21 mentions)
Acetonitrile, by Merck Group (19 mentions)
Acetonitrile, by Thermo Fisher Scientific (14 mentions)
Hplc grade methanol, by Merck Group (13 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (12 mentions)
Milli q system, by Merck Group (12 mentions)
Ammonium formate, by Merck Group (10 mentions)
Ms grade acetonitrile, by Thermo Fisher Scientific (9 mentions)
Ms grade methanol, by Thermo Fisher Scientific (9 mentions)
Methanol, by Thermo Fisher Scientific (9 mentions)
Hplc grade 2 propanol, by Thermo Fisher Scientific (8 mentions)
Hplc grade ammonium formate, by Merck Group (8 mentions)
Milli q water purification system, by Merck Group (6 mentions)
Lc ms grade acetonitrile, by Merck Group (5 mentions)
Acetonitrile, by Avantor (5 mentions)
Trichloroacetic acid, by Merck Group (4 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Acetic acid, by Merck Group (4 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (4 mentions)
130 protocols using hplc grade formic acid
1
HPLC-based Protein Quantification
2
HPLC-MS/MS Sample Preparation
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The HPLC grade acetonitrile (VWR), glacial acetic acid (Sigma-Aldrich, Darmstadt, Germany), HPLC grade formic acid (Merck, Darmstadt, Germany) and deionized water obtained from a Milli-Q Integral 5 water purification system (Millipore, MA, USA) were used for the sample preparation and LC–MS/MS analysis.
3
Quantification of Iridoid Glycosides
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Five reference compounds, including aucubin, geniposidic acid, geniposide, pinoresinol diglucoside, and syringaresinol diglucoside, were purchased from Chengdu Chroma-Biotechnology Co., Ltd. (Chengdu, China). The purities of all the above reference substances were more than 98% as determined by high-performance liquid chromatography (HPLC) analysis. HPLC-grade formic acid, methanol, and acetonitrile were purchased from Merck (Darmstadt, Germany).
4
Luteolin and Quercetin Extraction Protocol
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Luteolin (>98% purity) was purchased from Vicofarma® (São Paulo, Brazil). Quercetin (IS) (>98% purity) and 75–85% deacetylation degree low molecular weight chitosan (50,000–190,000 Da) were purchased from Sigma Aldrich® (St. Louis, MO, USA). HPLC-grade formic acid and methanol were purchased from Merck® (Darmstadt, Germany). HPLC-grade acetonitrile (ACN) was purchased from LiChrosolv® (Darmstadt, Germany). Monobasic potassium phosphate, sodium pyrophosphate, and oleic acid were purchased from Synth® (Diadema, Brazil). Hydrochloric acid and acetic acid were purchased from Vetec® (Duque de Caxias, Brazil). Ketamine and xylazine were purchased from Ceva® (Paulinia, Brazil). Sodium chloride, sodium hydroxide, ethylene glycol, and dibasic sodium phosphate were purchased from Biotec® (Lages, Brazil). Potassium chloride was purchased from Química Moderna® (São Paulo, Brazil). Tween 20 was purchased from Neon Comercial® (Suzano, Brazil). Ultrapure water was obtained by Millipore® purification system (Burlington, MA, USA).
5
Isotope-Labeled Metabolite Analysis
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CML, CEL, CML-d4 and CEL-d4 with 98% purity were obtained from Toronto Research Chemicals Inc. (TRC, Toronto, ON, Canada). Acrylamide-2,3,3-d3 with 98% purity was obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Acrylamide with 99% purity, HPLC grade formic acid and acetonitrile were obtained from Merck KGaA (Darmstadt, Germany), Thermo Fisher Scientific Inc. (Waltham, MA, USA) and Merck KGaA (Darmstadt, Germany), respectively. Amino acids standard solution was purchased from Waters Corporation (Waters, Milform, MA, USA).
6
Evaluation of Vasoprotective Compounds
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Chloral hydrate was purchased from Sinopharm Chemical Reagent Beijing Co., Ltd; adrenaline hydrochloride injection was purchased from Shanghai Harvest Pharmaceutical Co., Ltd.; cyasterone (C29H44O8, MW 520.65) and chikusetsusaponin IV (C47H74O18, MW 927.08) with a purity of 98% as determined by HPLC were purchased from SenBeijia Biological Technology Co., Ltd. (Nanjing, China).
Ethanol, petroleum ether, dichloromethane and n-butanol (chemical pure), mEthanol (HPLC grade) were supplied by Tedia Company Inc. (Fairfield, USA). HPLC-grade formic acid was purchased from Merck Company (Darmstadt, Germany). Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-6 (IL-6), nitric oxide (NO), tumor necrosis factor alpha (TNF-α), COX-2, TXA2, ET, MDA, eNOS, and SOD were obtained from Roche Diagnostics (Mannheim, Germany). HUVECs and thrombin were purchased from Yi Fei Xue Biotechnology (Nanjing, China). Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum were obtained from Invitrogen (Life Technologies Corporation, Carlsbad, CA, USA). All other reagents were of analytical grade, and distilled water was used to extract and prepare the samples.
Ethanol, petroleum ether, dichloromethane and n-butanol (chemical pure), mEthanol (HPLC grade) were supplied by Tedia Company Inc. (Fairfield, USA). HPLC-grade formic acid was purchased from Merck Company (Darmstadt, Germany). Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-6 (IL-6), nitric oxide (NO), tumor necrosis factor alpha (TNF-α), COX-2, TXA2, ET, MDA, eNOS, and SOD were obtained from Roche Diagnostics (Mannheim, Germany). HUVECs and thrombin were purchased from Yi Fei Xue Biotechnology (Nanjing, China). Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum were obtained from Invitrogen (Life Technologies Corporation, Carlsbad, CA, USA). All other reagents were of analytical grade, and distilled water was used to extract and prepare the samples.
7
Reagents and Solvents for Proteomic Analysis
8
Steroids Quantification in Plastic Containers
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All the detected compounds used in this paper, including the internal standard, were ordered from Sigma-Aldrich (Milano, Italy) with a purity > 99%. An individual stock solution of each compound was prepared by the dissolution of 0.5 mg of every single molecule in 0.5 mL of HPLC-grade methanol (Carlo Erba, Milano, Italy). Other solutions were obtained from the dilution of the stock solutions in methanol. HPLC-grade acetonitrile was purchased from Carlo Erba (Milano, Italy). HPLC-grade formic acid (99%) was obtained from Merck (Darmstadt, Germany). Deionized water (>18 MΩ cm resistivity) was obtained by purification of water with a Milli-Q SP system (Millipore, Bedford, MA, USA). Sterile test tubes were purchased from Becton–Dickinson (Franklin Lakes, NJ, USA). The analytical procedures were carried out in polypropylene vials, test tubes and plastic centrifuge tubes in order to preserve the concentration and stability of the hormones. The glass has demonstrated that it could interfere with those molecules as reported in the section “Evaluation of the stability of steroids in glass and plastic” (Results and Discussion, Table 3 ).
9
Buprofezin Quantification in Samples
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Analytical grade buprofezin (99.00% purity, Cas No. 69327-76-0) was procured from Dr. Ehrenstofer GmbH (Augsburg, Germany). Acetonitrile (HPLC grade) for the standard dilutions, sample extraction, and the LC mobile phase for instrumental analysis, and HPLC grade formic acid (>98%) for the LC mobile phase were purchased from Merck (Darmstadt, Germany). QuEChERS extraction packets for sample extraction and d-SPE tubes for sample purification were purchased from Agilent Technologies (Santa Clara, CA, USA). Combi-514R (Hanil Scientific Inc., Gimpo, Korea) and Combi-408 (Hanil Scientific Inc., Gimpo Korea) centrifuges were used for 50- and 2-mL centrifuge tubes, respectively. The 2010 Geno/Grinder® automated homogenizer (SPEX SamplePrep LLC, Metuchen, NJ, USA) was used for sample extraction. The 13-mm PTFE syringe filters were obtained from Advantec (Tokyo, Japan).
10
Multianalyte Pesticide Residue Analysis
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Analytical grade dinotefuran (98.5% purity), DN (98.5% purity), MNG (96.22% purity), UF (99.8% purity), fluazinam (99.89% purity), indoxacarb (96.1% purity), and thiacloprid (99.9% purity) were obtained from Dr. Ehrenstofer GmbH (Augsburg, Germany). Acetonitrile (HPLC grade) for standard dilutions, sample extraction, and the LC mobile phase were obtained from Merck (Darmstadt, Germany). HPLC-grade formic acid (> 98%) for the LC mobile phase was purchased from Merck (Darmstadt, Germany). Lead(II) acetate trihydrate was purchased from Daejung Chemicals (Iksan, Korea). The QuEChERS extraction packets and d-SPE tubes were obtained from Agilent Technologies (Santa Clara, CA, USA). Combi-514R (Hanil Scientific Inc., Gimpo, Korea) and Combi-408 (Hanil Scientific Inc., Gimpo Korea) centrifuges were used. A 2010 Geno/Grinder® automated homogenizer (SPEX SamplePrep LLC., Metuchen, NJ, USA) was used for sample extraction. The rotary evaporator used EYELA N-1000 (EYELA, Tokyo, Japan).
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