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51 protocols using ammonium molybdate tetrahydrate

1

Chitosan-Polyphosphate Biomaterials Synthesis

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Chitosan (Cs) 25 kDa was purchased from Heppe Medical Chitosan GmbH (Halle, Germany) and sodium polyphosphate (PP) from Merck KGaA (Vienna, Austria). Alkaline phosphatase from bovine intestinal mucosa (7165 units/mg protein), ammonium molybdate tetrahydrate (8–83%), glucose-D-(+) ≥ 99.5% anhydrous, magnesium chloride anhydrous (MgCl2) ≥ 98%, minimum essential medium (MEM) eagle, malachite green oxalate salt 90%, potassium phosphate monobasic (KH2PO4) ≥ 99.5%, phosphatase inhibitor cocktail 2, resazurin sodium salt, rhodamine 123, Triton X-100, and anhydrous zinc chloride (ZnCl2) ≥ 97% were purchased from Sigma-Aldrich (Vienna, Austria). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ≥ 99.5% was purchased from ROTH GmbH (Karlsruhe, Germany). Fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were purchased from Biochrom, Merck (Tutzing, Germany). D-(+)-Trehalose dehydrate ≥98.0% was purchased from TCI Chemicals (Eschborn, Germany).
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2

Evaluation of Soybean-Derived Phospholipids

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Soybean-derived L-alpha-phosphatidylcholine (PC – purity grade ≥97%), A (purity ≥97%), Q (purity ≥95%), K (purity ≥90%), 1,1-diphenyl-2-picrylhydrazyl (DPPH), n-(1-naphthyl)ethylenediamine dihydrochloride, ammonium molybdate tetrahydrate, insulin from bovine pancreas, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Sodium nitroprusside was purchased from Fluka Chemie AG (Buchs, St Gallen, Switzerland) and sulfanilamide from Friendemann Schmidt (CT Parkwood, Western Australia, Australia). William’s E medium (without phenol red and glutamine) and Glutamax I were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum, penicillin−streptomycin, and trypsin were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). Ascorbic acid (AA), hydrocortisone hemisuccinate, and Triton X-100 were obtained from Kollin Chemicals (Malaysia), Santa Cruz Biotechnology Inc. (Dallas, TX, USA), and Alfa Aesar (Heysham, Lancashire, UK), respectively. Dichloromethane (DCM), tetrahydrofuran, dimethylsulfoxide (DMSO), hexane, chloroform, EA, and n-butanol were obtained from EMSURE® (Darmstadt, Germany). All chemical reagents were of analytical grade unless otherwise stated; extraction solvents were of HPLC/LC–MS grade, purchased from EMD Millipore (Billerica, MA, USA).
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3

Synthesis and Characterization of MoS2 Nanoparticles

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Ammonium molybdate tetrahydrate (99.98%, Sigma-Aldrich, Burlington, MA, USA), thiourea (AMRESCO, Solon, OH, USA), and polyvinylpyrrolidone (Sigma-Aldrich, Burlington, MA, USA) were used to synthesize the MoS2 NPs. Potassium hexacyanoferrate (III) (K3Fe(CN)6) (approx. 99.0%, Sigma-Aldrich, Burlington, MA, USA) and potassium hexacyanoferrate (II) trihydrate (K4Fe(CN)6) (≥99.0%, Sigma-Aldrich, Burlington, MA, USA) solution in phosphate-buffered saline (PBS) (Sigma-Aldrich, Burlington, MA, USA) was used as the electrolyte in this study. EDC and NHS were purchased from Thermo Scientific (≥99.0%, Waltham, MA, USA). Human serum (Sigma-Aldrich, Burlington, MA, USA), cysteamine (Cys) (≥98.0%, Sigma-Aldrich, Burlington, MA, USA), gp120 antibody (Sino biological, Wayne, PA, USA), and gp120 antigen (ACRO Biosystem, Newark, DE, USA) were used to fabricate the biosensor. All aqueous solutions were prepared using deionized (DI) water from a Millipore Milli-Q water purifier operating at a resistance of 18 MΩ·cm. Myoglobin (Mb) (≥90.0%, Sigma-Aldrich, Burlington, MA, USA), hemoglobin (Hb) (Sigma-Aldrich, Burlington, MA, USA), thioredoxin (Trx) (Sino biological, Wayne, PA, USA), and prostate-specific antigen (PSA) (Abcam, Cambridge, UK) were used to investigate the selectivity of the fabricated biosensor.
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4

Polymyxin B Sulfate and Sodium Polyphosphate Protocol

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Polymyxin B sulfate was
purchased from Molekula GmbH (Munich, Germany) and sodium polyphosphate
(Graham’s salt) was purchased from Merck KGaA (Vienna, Austria).
Alkaline phosphatase from bovine intestinal mucosa (IAP, 7165 units/mg
protein), ammonium molybdate tetrahydrate (81–83%), glucose-d-(+) ≥ 99.5% anhydrous, hydrochloric acid, sodium hydroxide,
minimum essential medium (MEM) eagle, malachite green (MLG), oxalate
salt 90%, potassium phosphate monobasic (KH2PO4) ≥ 99.5%, phosphatase inhibitor cocktail 2 (PIC), resazurin
sodium salt, Triton X-100, lipase from porcine pancreas and fluorescein
isothiocyanate isomer 1 (FITC) 90% were purchased from Sigma-Aldrich
(Vienna, Austria). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) ≥ 99.5% was obtained from ROTH GmbH (Karlsruhe, Germany). d-(+)-Trehalose dihydrate >98.0% was purchased from TCI Chemicals
(Eschborn, Germany). 2,4,6-Trinitrobenzenesulfonic acid (TNBS) was
purchased from Chemos, Germany.
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5

Heterogeneous Catalysis Precursor Synthesis

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Nickel(ii) nitrate hexahydrate (Ni(NO3)2·6H2O) (>98.5% purity), ammonium molybdate tetrahydrate ((NH4)6Mo7O24·4H2O) (81.0–83.0% purity MoO3 basis), Brij 30, ammonium sulphide solution (20.0% in H2O), tetraethyl orthosilicate (TEOS) (98.0% purity), sucrose (99.5% purity), sulphuric acid (99.9% purity), dodecane (99.0% purity), guaiacol (99.0% purity), catechol (99.0% purity), anisole (99.7% purity), cyclohexanol (99.0% purity), cyclohexane (99.5% purity), cyclohexene (analytical grade), veratrole (99.0% purity), cresol (99.0% purity), pluronic P-123, activated carbon (norit SX ultra) were all purchased from Sigma-Aldrich.
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6

Synthesis and Characterization of Lithium-Ion Battery Materials

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All chemicals were of analytical grade and used directly without any purification. Ammonium molybdate tetrahydrate ((NH4)6Mo7O24·4H2O), lithium cobalt oxide (LiCoO2), concentrated HNO3 (70 wt%), n-butyl lithium in hexane, hexane, Li foil, graphite flakes (average size 20 µm), sodium alginate, Super P carbon black, and 2 M LiPF6 in ethyl methyl carbonate were purchased from Sigma-Aldrich.
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7

Synthesis of Graphene-Based Nanomaterials

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Ammonium molybdate tetrahydrate, thiourea, ethanol, cobalt(iii) acetylacetonate, iron(iii) acetylacetonate, oleylamine, and pure graphite powder (99.99%) were purchased from Sigma Aldrich. HNO3, H2SO4, and KClO3 were procured from Fisher Scientific. All chemicals were reagent grade and were used as received without further purification. High purity deionized (DI) water was used in this work as a solvent and for purification.
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8

Catalytic Oxidation of Castor Oil

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OA (90%), castor oil, hexanes (ACS Grade),
nickel nitrate hexahydrate [Ni(NO3)2·6H2O], ammonium molybdate tetrahydrate [(NH4)6Mo7O24·4H2O], and magnesium sulfate
heptahydrate [MgSO4·7H2O] were obtained
from Sigma-Aldrich, Canada, and are used as received. Alumina (γ-Al2O3) powder (Catalox SSCa 5/200) was obtained from
SASOL. Frying oil (used canola oil) was obtained from author’s
home and filtered to remove solid particles before use. Palm oil was
obtained from Malaysia. A compact ultrapure water system (EASY pure
LF, Mandel Scientific Co., model BDI-D7381) was used to obtain deionized
water (18.2 MΩ).
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9

Phytase-Mediated Phytate Hydrolysis

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Potassium chloride (>99 %), alginic acid sodium salt from brown algae (medium viscosity), phytase from wheat (>0.01 unit/mg solid, 6-phytase), sodium hydroxide anhydrous (>97 %), calcium chloride dihydrate (>99.5 %), ammonium molybdate tetrahydrate, ethylene diamine tetra-acetic acid (EDTA) disodium salt (>99 %), trichloroacetic acid (TCA), fluorescein isothiocyanate isomer I (FITC), and l-ascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO, US). Hydrochloric acid and PierceTM rapid gold BCA (bicinchoninic acid) protein assay kits were purchased from Thermo Fisher Scientific (Liverpool, NY, US). Pepsin (min. 3,000 units/mg) was purchased from Mallinckrodt Chemicals (Dublin, Ireland). Sodium phytate (95 %) was purchased from Astatech Inc. (Bristol, PA, US). κ-Carrageenan was purchased from TIC GUMS (Westchester, IL, US). Chitosan from Aspergillus niger was purchased from Sarchem Laboratories (Farmingdale, NJ, US). Sulfuric acid (95–98 %) was purchased from Fluka Honeywell (Charlotte, NC, US).
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10

Extractable Phosphorus Determination Protocol

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Determination of extractable phosphorus was modified from previous study [1 (link)]. Briefly, 5 g of each sample were mixed with 50 mL 0.2% pepsin solution, which was prepared with 0.2 g pepsin (Sigma, St. Louis, MO, USA) dissolved in 100 mL 0.1 M HCl solution. After 3 h of incubation at 37°C and 80 rpm, the mixtures were centrifuged at 3,000 rpm, followed by filtration through Advantec No.1 filter papers. The 0.3 mL extracts were added to 0.6 mL deionized water and 0.6 mL vanadium-ammonium molybdate solution, which was prepared with nitric acid, ammonium metavanadate (Sigma, USA) and ammonium molybdate tetrahydrate (Sigma, USA). Then, the mixtures were centrifuged again at 6,000 rpm for 10 min, and the absorbance of suspension was detected at 415 nm. Solutions of 0.3125 to 2.5 μmol/mL potassium dihydrogen phosphate were also detected to draw a standard curve. The 300 g WB treated with 750-unit Phy was also analyzed.
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