To activate the surface, the device was first treated with oxygen plasma for 45 s at 100 W followed by infusion of 2% solution of (3-aminopropyl) trimethoxysilane (Sigma). The 2% (3-aminopropyl) trimethoxysilane is prepared in 200 proof anhydrous ethanol (Sigma). Then, the device was washed with 140 proof ethanol followed 200 proof ethanol (Koptec) and then left to dry at 60 °C for a few hours in a convection oven. Rat tail type I collagen (Corning) was diluted in PBS and amine coupling reagent, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (20 mg ml−1; Sigma) in a 1:1 ratio to reach a final concentration of 100 μg ml−1 and infused into the device. The devices were incubated at 37 °C for 3–4 h and then washed with 1% bovine serum albumin solution followed by saline or PBS, before blood perfusion.
3 aminopropyltrimethoxysilane
Manufactured by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, United Kingdom, France
3-aminopropyltrimethoxysilane is a silane compound used as a coupling agent in various applications. It serves as a molecular bridge, facilitating the attachment of organic compounds to inorganic materials. This substance is commonly utilized in the manufacturing and modification of materials, coatings, and surfaces.
Automatically generated - may contain errors
Lab products found in correlation
Glutaraldehyde, by Merck Group (14 mentions)
Tetraethyl orthosilicate, by Merck Group (12 mentions)
Sodium hydroxide (naoh), by Merck Group (10 mentions)
Ammonium persulfate, by Merck Group (9 mentions)
Toluene, by Merck Group (8 mentions)
Ammonium hydroxide, by Merck Group (8 mentions)
Acetic acid, by Merck Group (7 mentions)
Sulfo sanpah, by Thermo Fisher Scientific (7 mentions)
Methanol, by Merck Group (6 mentions)
Ethanol, by Merck Group (6 mentions)
Hydrochloric acid (hcl), by Merck Group (5 mentions)
Triethylamine, by Merck Group (5 mentions)
Tetrahydrofuran, by Merck Group (5 mentions)
Silver nitrate, by Merck Group (5 mentions)
N n methylenebisacrylamide, by Merck Group (5 mentions)
3 mercaptopropyl trimethoxysilane, by Merck Group (4 mentions)
Bis acrylamide, by Merck Group (4 mentions)
Acrylamide, by Merck Group (4 mentions)
Hydrogen peroxide, by Merck Group (4 mentions)
N hydroxysuccinimide, by Merck Group (4 mentions)
112 protocols using 3 aminopropyltrimethoxysilane
1
Surface Activation and Collagen Coating
2
Surface Modification of MgO with Aminosilane
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A portion of 2 g of MgO (first dried overnight at 100 °C) was put into a glass flask. Then 200 cm3 of dried toluene and 2.5 cm3 of (3-aminopropyl)trimethoxysilane (Aldrich, St. Louis, MO, USA) were introduced. The synthesis mixture was then stirred and heated at 110 °C for 18 h. The solid product was then filtered off and washed with 300 cm3 of toluene and finally dried at 80 °C for 24 h. The sample obtained is denoted as NH2/MgO.
3
Silanes Modified Clay Suspension Preparation
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Example 4
Preparation of Silanes Modified Clay Suspension (SMMT[4])
5.0 g of pristine clay (montmorillonite) obtained from Nanocor Inc. of Arlington Heights of Illinois of the United States of America was mixed with 125 ml deionised water and stirred for 6 hours, followed by ultrasonication in a water-bath for 30 minutes. Then, 0.1875 ml of acetic acid was added to the solution and stirred for another 12 hours. After that, 0.3 g of (3-aminopropyl)trimethoxysilane (97%, Aldrich) was first slowly injected (0.1 ml/min) into the clay suspension, followed by 0.45 g of (3-glycidoxypropyl)trimethoxysilane (3 98%, Aldrich) under high speed of homogenizing process at 15,000 rpm by using an IKA T18 Basic Ultra Turrax for 15 minutes. Then, silanes modified clay suspension was heated at 50° C. for 6 hours under stirring.
4
Silanes Modified Clay Suspension Preparation
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Example 3
Preparation of Silanes Modified Clay Suspension (SMMT[3])
5.0 g of pristine clay (montmorillonite) obtained from Nanocor Inc. of Arlington Heights of Illinois of the United States of America was mixed with 125 ml deionised water and stirred for 6 hours, followed by ultrasonication in a water-bath for 30 minutes. Then, 0.1875 ml of acetic acid was added to the solution and stirred for another 12 hours. After that, 0.2 g of (3-aminopropyl)trimethoxysilane (97%, Aldrich) was first slowly injected (0.1 ml/min) into the clay suspension, followed by 0.3 g of (3-glycidoxypropyl)trimethoxysilane (3 98%, Aldrich) under high speed of homogenizing process at 15,000 rpm by using an IKA T18 Basic Ultra Turrax for 15 minutes. Then, silanes modified clay suspension was heated at 50° C. for 6 hours under stirring.
5
Silica Wafer Surface Modification
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Silica
wafers (2.0 cm × 2.0 cm) were
treated in a mixture of H2SO4 and 30% H2O2 (volumetric ratio = 7:3) for 30 min, rinsed
with deionized water, and
dried under nitrogen gas (N2) flow. The N-Isopropylacrylamide (NIPAAm) monomer was purchased from J&K
Chemical, N,N,N′,N″,N″-pentamethyldiethylenetriamine
(PMDETA) was purchased from TCI. Trichloro(octyl)silane (TCOS), octadecyltrichlorosilane
(OTCS), 3-aminopropyl trimethoxysilane (APTMS), 3-mercaptopropyl trimethoxysilane
(MPTMS), trichloro(1H,1H,2H,2H-perfluorooctyl) silane (PFS), 2-bromoisobutyryl
bromide, and copper(I) chloride (CuCl) were provided by Aldrich. The
polystyrene microparticles with a diameter of 620 nm were provided
by Wuhan Sphere Scientific Co., Ltd. and were cleaned with water and
50% ethanol several times before use. Dichloromethane, absolute ethanol,
triethylamine, sodium dodecyl sulfate (SDS), methanol, 1,2-dichloroethane,
and the four components of the photoresist were used directly after
received. The masks employed for photolithography were machined by
the Institute of Microelectronics of Chinese Academy of Sciences.
The TJ-1A-Micro Flow syringe pump was purchased from Baoding Longer
Precision Pump Co., Ltd. The water in all experiments was deionized
and doubly distilled prior to use.
wafers (2.0 cm × 2.0 cm) were
treated in a mixture of H2SO4 and 30% H2O2 (volumetric ratio = 7:3) for 30 min, rinsed
with deionized water, and
dried under nitrogen gas (N2) flow. The N-Isopropylacrylamide (NIPAAm) monomer was purchased from J&K
Chemical, N,N,N′,N″,N″-pentamethyldiethylenetriamine
(PMDETA) was purchased from TCI. Trichloro(octyl)silane (TCOS), octadecyltrichlorosilane
(OTCS), 3-aminopropyl trimethoxysilane (APTMS), 3-mercaptopropyl trimethoxysilane
(MPTMS), trichloro(1H,1H,2H,2H-perfluorooctyl) silane (PFS), 2-bromoisobutyryl
bromide, and copper(I) chloride (CuCl) were provided by Aldrich. The
polystyrene microparticles with a diameter of 620 nm were provided
by Wuhan Sphere Scientific Co., Ltd. and were cleaned with water and
50% ethanol several times before use. Dichloromethane, absolute ethanol,
triethylamine, sodium dodecyl sulfate (SDS), methanol, 1,2-dichloroethane,
and the four components of the photoresist were used directly after
received. The masks employed for photolithography were machined by
the Institute of Microelectronics of Chinese Academy of Sciences.
The TJ-1A-Micro Flow syringe pump was purchased from Baoding Longer
Precision Pump Co., Ltd. The water in all experiments was deionized
and doubly distilled prior to use.
6
Mussel-Inspired Polymer Synthesis
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All chemical reagents were purchased from Sigma-Aldrich Co. (St Louis, MO, USA), including Mussel, HCl, NaOH, (3-aminopropyl)trimethoxysilane (APMS), ethyl alcohol, acidic acid, 2-(dodecylsulfanylthiocarbonylsulfanyl)-2-methylpropionic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N,N-dimethylformamide (DMF), N-(2-(acryloyloxy) ethyl)-N,N-dimethyl, hexyl bromide, methyl cyanide, azobisisobutyronitrile (AIBN), and 2,2,3,3,4,4,4-heptafluorobutyl acrylate, 97%.
7
Synthesis and Characterization of Magnetic Nanoparticles
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Toluene (99.8%), iron(II) chloride tetra hydrate (FeCl2.4H2O, >99%) (3-Aminopropyl) trimethoxysilane (APTMS, 97%), ammonia (25%), methanol (≥99.9%), tetraethyl orthosilicate (TEOS, ≥99.0%), hydrochloric acid (37%), nitric acid (≥65%) (ethanol (99%), sodium hydroxide (≥98%), iron(III) chloride hexahydrate (FeCl3.6H2O, 99%), and acetone (99.5%) were purchased from Merck (Darmstadt, Germany). Molecularsieve (4 Å beads 8–12 mesh), isopropyl alcohol ((2-propanol) ≥99.7%), triethylamine (TEA), and 1,8–bis(3-chloropropoxy) anthracene-9,10-dione (BCAD) were supplied from Sigma-Aldrich. 1000 mg/L metal standard solutions in 0.5% HNO3 were purchased from Merck.
Transmission Electron Microscopy (TEM, JEM-2100F (JEOL), X-ray diffraction (XRD, Bruker-D8 Advance with Davinci), Scanning Electron Microscope (SEM, HITACHI (SU5000), and Fourier-Transform Infrared Spectroscopy (FTIR, Bruker Vertex 70 ATR-FTIR) were used to characterize the nanoparticle samples generated in each stage. The pH of the studies was adjusted using a Jenway 3010 digital pH meter. Using an Inductively Coupled Plasma Optical Emission Spectrometry, the residue copper(II) concentrations in the solutions after adsorption were determined (ICP-OES, Agilent-720).
Transmission Electron Microscopy (TEM, JEM-2100F (JEOL), X-ray diffraction (XRD, Bruker-D8 Advance with Davinci), Scanning Electron Microscope (SEM, HITACHI (SU5000), and Fourier-Transform Infrared Spectroscopy (FTIR, Bruker Vertex 70 ATR-FTIR) were used to characterize the nanoparticle samples generated in each stage. The pH of the studies was adjusted using a Jenway 3010 digital pH meter. Using an Inductively Coupled Plasma Optical Emission Spectrometry, the residue copper(II) concentrations in the solutions after adsorption were determined (ICP-OES, Agilent-720).
8
Chiral Amines and Silica Nanoparticle Synthesis
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Phosphoryl chloride (POCl3, 99%), (R)-(+)-α-methylbenzylamine (RMBA, 98%), (S)-(−)-α-methylbenzylamine (SMBA, 98%) and (±)-α-methylbenzylamine (MBA, 98%) were bought from Sigma-Aldrich. Methanol, ethanol, tetrahydrofuran (THF), sodium chloride (NaCl), ferric chloride hexahydrate (FeCl3·6H2O), ferrous chloride tetrahydrate (FeCl2·4H2O), ammonium hydroxide (25% (w/w)), glycerol, tetraethyl orthosilicate (TEOS), 3-aminopropyltrimethoxysilane (APTMS), dl -tartaric acid (99%), dl -phenylalanine (99%), dl -valine (99%) and triethylamine (TEA, 99%) were purchased from Merck.
9
Carbon Fiber Reinforced Epoxy Composites
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Pitch-based CFs used in
this study were DIALEAD CARBON FIBER: K223HM distributed by Mitsubishi
Chemicals, Inc., with a density of 2.2 g/cm3, an average
length of 50–200 μm, a tensile modulus of 900 MPa, and
a tensile strength of 3.8 GPa. The epoxy precursor bisphenol A diglycidyl
ether with a purity of 100% and trimethylolpropane tris[poly(propylene
glycol), amine terminated] ether (T-403) with an average Mn of 440
g/mol were supplied by MilliporeSigma and used as received. Sulfuric
acid (ACS reagent, 95.0–98.0%), nitric acid (ACS reagent, ≥90.0),
ethanol (ACS reagent, >95% pure), and (3-aminopropyl) trimethoxy
silane
(97%) were supplied by MilliporeSigma.
this study were DIALEAD CARBON FIBER: K223HM distributed by Mitsubishi
Chemicals, Inc., with a density of 2.2 g/cm3, an average
length of 50–200 μm, a tensile modulus of 900 MPa, and
a tensile strength of 3.8 GPa. The epoxy precursor bisphenol A diglycidyl
ether with a purity of 100% and trimethylolpropane tris[poly(propylene
glycol), amine terminated] ether (T-403) with an average Mn of 440
g/mol were supplied by MilliporeSigma and used as received. Sulfuric
acid (ACS reagent, 95.0–98.0%), nitric acid (ACS reagent, ≥90.0),
ethanol (ACS reagent, >95% pure), and (3-aminopropyl) trimethoxy
silane
(97%) were supplied by MilliporeSigma.
10
Hepatocyte Culture on Tunable Hydrogels
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Circular, 22 mm, glass coverslips (VWR) were activated by 0.5% 3-aminopropyl trimethoxysilane and glutaraldehyde (MilliporeSigma). Different ratios of 40% acrylamide and 2% bis-acrylamide (Bio-Rad) were combined to generate 140 Pa, 1 kPa, and 6 kPa stiffness gels as calibrated previously (83 (link)). Polymerization was initiated by tetramethylethylenediamine (Bio-Rad) and potassium persulfate (MilliporeSigma). Then 0.01% bis-acrylamide, 0.002% di(trimethylolpropane) tetraacrylate (MilliporeSigma), 0.025% Irgacure (BASF Resins), 0.006% acrylic acid N-hydroxysuccinimide ester (MilliporeSigma), and 25% ethanol in 50 mM HEPES (pH 6.0) were applied, and gels were exposed to 245 nm ultraviolet light for 300 seconds for photoactivation. Gels were thoroughly washed in phosphate-buffered saline to remove excess reagent and incubated with 150 mg/mL rat tail collagen I (VWR) solution overnight at 4°C for conjugation. Before cells were plated, the gels were equilibrated in DMEM (Mediatech) at 37°C overnight. Primary hepatocytes were cultured at 50,000 cells/gel/well in 12-well plates (Eppendorf) in DMEM supplemented with 5% FBS (Hyclone), l -glutamine, antibiotics, insulin-transferrin-selenium, and HEPES (Mediatech).
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