Dms114

Manufactured by American Type Culture Collection

Sourced in United States, Germany

The DMS114 is a modular automated pipetting robot designed for high-throughput liquid handling tasks. It is equipped with a precision dispenser capable of handling volumes ranging from 0.5 μL to 5,000 μL. The DMS114 automates repetitive pipetting operations, improving efficiency and reducing manual errors in laboratory workflows.

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Lab products found in correlation

46 protocols using dms114

Characterization of Engineered Osteosarcoma and Lung Cancer Cells

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The human osteosarcoma cell line (U2OS), and the small cell lung cancer (SCLC) cell line (DMS114) were obtained from the American Type Culture Collection (ATCC). The non-small lung cancer cell line (HCC15) was supplied by the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ). U2OS cell lines stably transfected with pcDNA3.1 vector containing the sequence encoding the full-length FGFR1 (U2OSR1) or empty pcDNA3.1 vector (U2OS) were prepared as described previously (16 (link)). The U2OS cell line stably transfected with FGFR1-IIIc_K514R (U2OSR1-K514R) was kindly provided by Dr. Ellen M. Haugsten from the Department of Molecular Cell Biology, Institute for Cancer Research (Oslo University Hospital). U2OS, U2OSR1, and U2OSR1-K514R cells were cultured in DMEM (Biowest) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin and 1 mg/ml geneticin). DMS114 cells grew in Waymouth’s MB 752/1 medium (ATCC) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and antibiotics (100 U/mL penicillin, 100 µg/mL streptomycin). HCC15 cells were cultured in RPMI 1640 Medium (Biowest) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and antibiotics (100 U/mL penicillin, 100 µg/mL streptomycin). All cancer cell lines were kept at 37 °C in a 5% CO₂ incubator.

Szymczyk J., Sochacka M., Chudy P., Opalinski L., Otlewski J, & Zakrzewska M. (2022). FGF1 protects FGFR1-overexpressing cancer cells against drugs targeting tubulin polymerization by activating AKT via two independent mechanisms. Frontiers in Oncology, 12, 1011762.

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Cell Culture Protocols for Cancer Research

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DMS114 (human
small cell lung cancer, ATCC CRL-2066)
cells were cultured in Waymouth’s MB 752/1 from Gibco (Waltham,
MA). U2OS (human osteosarcoma, HTB-96) and U2OS stably transfected
with FGFR1 (U2OS-R1) were cultured in DMEM High Glucose with stable
glutamine and sodium pyruvate from Biowest (France). NCI-H520 (human
squamous cell carcinoma, ATCC HTB-182) and HCC15 (human squamous cell
lung carcinoma) and were cultured in RPMI-1640 medium from Gibco (Waltham,
MA). All media were supplemented with 10% fetal bovine serum from
Thermo Fisher Scientific, and 1% penicillin/streptomycin mix was from
Biowest (France). Additionally, the U2OS-R1 cell medium contained
50 μg/mL gentamicin sulfate from Thermo Fisher Scientific. All
cell lines were cultured in a humidified incubator at 37 °C in
a 5% CO₂ atmosphere. The DMS114, NCI-H520, and U2OS cell
lines were obtained from American Type Culture Collection (Manassas,
VA). HCC15 cells were supplied by the Leibniz Institute DSMZ, German
Collection of Microorganisms and Cell Cultures. The U2OS cells stably
expressing FGFR1 (U2OS-R1) were a kind gift from Dr. Ellen M. Haugsten
from The Norwegian Radium Hospital.^{38 (link)} The E. coli expression strain Rosetta 2(DE3)pLysS was from Novagen-EMD
Biosciences (Madison, WI).

Krzyscik M.A., Zakrzewska M, & Otlewski J. (2020). Site-Specific, Stoichiometric-Controlled, PEGylated Conjugates of Fibroblast Growth Factor 2 (FGF2) with Hydrophilic Auristatin Y for Highly Selective Killing of Cancer Cells Overproducing Fibroblast Growth Factor Receptor 1 (FGFR1). Molecular Pharmaceutics, 17(7), 2734-2748.

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Cell Line Culture and Reagent Details

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DU145 (ATCC, HTB-81™) and DU145-SLFN11 KO cells were grown in DMEM medium with 10% FBS/1% penicillin-streptomycin. MOLT-4 (ATCC, CRL-1582™), MOLT-4-SLFN11 KO, CCRF-CEM (ATCC, CCL-119™), CCRF-CEM-SLFN11 KO, DMS114 (ATCC, CRL-2066™), DMS114-SLFN11 KO, and HCT-116 (ATCC, CCL-247™) cells were grown in RPMI1640 medium with 10% FBS/1% penicillin-streptomycin. DU145, MOLT-4, CCRF-CEM, DMS114, and HCT-116 were purchased from the ATCC. All SLFN11 KO cells were established in our laboratory (30 (link)). DT40, DT40-BRCA1 KO, and DT40-BRCA2 KO cells (gifts from Dr. S. Takeda, Kyoto University, Kyoto, Japan) were grown in RPMI1640 medium supplemented with 1% chicken serum, 10 nmol/L β-mercaptoethanol, 10% FBS, and 1% penicillin-streptomycin. UWB1.289 and UWB1.289+BRCA1 cells (gifts from Dr. J. Lee, NCI) were cultured in complete growth medium (50% RPMI-1640 medium, 50% MEGM medium with 10% FBS/1% penicillin-streptomycin. CPT, exatecan, topotecan, SN-38, LMP400, talazoparib and ceralasertib (AZD6738) were acquired from the Developmental Therapeutics Program (DCTD, NCI). CBX-12 is obtained from Cybrexa Therapeutics. All cell lines were passaged 15 times and examined by MycoAlert Mycoplasma Detection Kit (Lonza).

Jo U., Murai Y., Agama K.K., Sun Y., Saha L.K., Yang X., Arakawa Y., Gayle S., Jones K., Paralkar V., Sundaram R.K., Doorn J.V., Vasquez J.C., Bindra R.S., Choi W.S, & Pommier Y. (2022). TOP1-DNA trapping by exatecan and combination therapy with ATR inhibitor. Molecular cancer therapeutics, 21(7), 1090-1102.

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Cell Line Maintenance and Inhibitor Evaluation

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DMS114, NCI-H1581, NCI-H1703, NCI-H2170, and NCI-H520 cell lines were obtained from ATCC. NCI-H1581 cells were routinely maintained in DMEM/F12; DMS114 in Waymouth’s MB752/1; whereas NCI-H1703, NCI-H2170, and NCI-H520 were maintained in RPMI 1640 medium. All culture media contained 10% of FBS and penicillin/streptomycin (100 U/mL/100 μg/mL). Cells were grown at 37 °C in a humidified atmosphere of 5% CO₂. All culture media and corresponding supplements were purchased from Merck KGaA (Darmstadt, Germany) or Biowest (Riverside, MO, USA). CPL304110 (WO/2014/141015) inhibitor was provided by Celon Pharma S.A., Poland [17 (link)]. SB202190 and SB203580 inhibitors were purchased from Selleckchem (Houston, TX, USA).

Zarczynska I., Gorska-Arcisz M., Cortez A.J., Kujawa K.A., Wilk A.M., Skladanowski A.C., Stanczak A., Skupinska M., Wieczorek M., Lisowska K.M., Sadej R, & Kitowska K. (2021). p38 Mediates Resistance to FGFR Inhibition in Non-Small Cell Lung Cancer. Cells, 10(12), 3363.

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Cell Line Maintenance for Cancer Research

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SCLC cell lines H446, H196, H524, DMS114, DMS79, H187, H889, and HT-29, and colorectal adenocarcinoma cell line Colo205 were purchased from ATCC and maintained in culture. Cell lines were authenticated using short tandem repeat DNA profiling and tested for mycoplasma contamination before experiments. Cells were cultured at 37°C and 5% CO2 in RPMI-1640 supplemented with 10% FBS, except HT-29 and DMS-114, which were grown in McCoy’s 5a Medium Modified supplemented with 10% FBS and Waymouth’s MB 75/21 medium supplemented with 10% FBS respectively. Colo205 cells were cultured in DMEM with 10% fetal bovine serum at 37°C and 10% CO2.

Thomas A., Takahashi N., Rajapakse V.N., Zhang X., Sun Y., Ceribelli M., Wilson K.M., Zhang Y., Beck E., Sciuto L., Nichols S., Elenbaas B., Puc J., Dahmen H., Zimmermann A., Varonin J., Schultz C.W., Kim S., Shimellis H., Desai P., Klumpp-Thomas C., Chen L., Travers J., McKnight C., Michael S., Itkin Z., Lee S., Yuno A., Lee M.J., Redon C.E., Kindrick J.D., Peer C.J., Wei J.S., Aladjem M.I., Figg W.D., Steinberg S.M., Trepel J.B., Zenke F.T., Pommier Y., Khan J, & Thomas C.J. (2021). Therapeutic targeting of ATR yields durable regressions in small cell lung cancers with high replication stress. Cancer cell, 39(4), 566-579.e7.

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SCLC Cell Lines and PDX Cultivation

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NCI‐H211, NCI‐H524, DMS‐114, NCI‐H841, NCI‐H1048, NCI‐H1341, NCI‐H446, NCI‐H146, NCI‐H889, and U2OS DRGFP (Nakanishi et al, 2011 (link)) cell lines were purchased from ATCC. NCI‐H211, NCI‐H889, NCI‐H1048, NCI‐H1341, and U2OS DRGFP cell lines are female and the rest are male, additional information for cell lines can be observed in Table 1. Cell lines were authenticated using short tandem repeat analysis, and were monthly tested for mycoplasma contamination. PDX‐03 and PDX‐06 were derived from a male and a female SCLC patient, respectively. Cell medium was RPMI‐1640 supplemented with 10% FBS for all lines to maintain consistency. DT40 (chicken cell lines) were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with FBS 10% and chicken serum 5%. Cells were grown at 37°C and 5% CO₂.

Schultz C.W., Zhang Y., Elmeskini R., Zimmermann A., Fu H., Murai Y., Wangsa D., Kumar S., Takahashi N., Atkinson D., Saha L.K., Lee C., Elenbaas B., Desai P., Sebastian R., Sharma A.K., Abel M., Schroeder B., Krishnamurthy M., Kumar R., Roper N., Aladjem M., Zenke F.T., Ohler Z.W., Pommier Y, & Thomas A. (2023). ATR inhibition augments the efficacy of lurbinectedin in small‐cell lung cancer. EMBO Molecular Medicine, 15(8), e17313.

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Lung Cancer Cell Line Characterization

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Non-small cell lung cancer cell lines A549, H1299, and SCLC cell lines DMS114, H446, H1688 and H69AR were obtained from ATCC.

Zhang S., Chao H.H., Wang X., Zhang Z., Lee E.Y, & Lee M.Y. (2018). Loss of the p12 subunit of DNA Polymerase Delta Leads to a Defect in HR and Sensitization to PARP Inhibitors. DNA repair, 73, 64-70.

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Cell Line Cultivation and Validation

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DU145, CCRF-CEM and MOLT4 were obtained from DCTD, DTP (3 (link),30 (link)). DMS114, HEK293 and 293T were purchased from ATCC (Virginia, USA). Li-7 was bought from ElabScience (Texas, USA). DU145, HEK293 and 293T cell lines were grown in DMEM medium (11995065; GIBCO) with 10% fetal bovine serum and 1% penicillin-streptomycin. CCRF-CEM, MOLT4, DMS114 and Li-7 cell lines were grown in RPMI medium 1640 (11875093; GIBCO) added with 10% FBS and 1% penicillin-streptomycin at 37°C in 5%CO₂. Cell lines in this study were passaged about 15 times; all cell lines were tested by MycoAlert™ Mycoplasma Detection Kit (LT07-418; Lonza). Reagents are listed in the Supplementary Table 1.

Murai Y., Jo U., Murai J., Jenkins L., Huang S.Y., Chakka S., Chen L., Cheng K., Fukuda S., Takebe N, & Pommier Y. (2021). SLFN11 inactivation induces proteotoxic stress and sensitizes cancer cells to ubiquitin activating enzyme inhibitor TAK-243. Cancer research, 81(11), 3067-3078.

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Lung Cancer Cell Line Transfection

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Lung adenocarcinoma cell lines A549 and PC9, human large cell lung cancer cell line H460, human lung squamous cell carcinoma cell line H520, and human small cell lung cancer cell lines DMS53 and DMS114 were purchased from ATCC (Manassas, VA, USA). The lung adenocarcinoma cell line II18 was purchased from RIKEN Cell Bank (Riken, Tsukuba, Japan). These cells were cultured at 37°C with 5% CO₂ in RPMI-1640 medium (Wako, Japan) containing 10% fetal calf serum. The tumor cells were transfected with a CARD9 siRNA (Thermo Fisher Scientific, MA) or with Silencer^® Select Negative Control No. 1 siRNA (Thermo Fisher Scientific, MA), which had no effect on any gene expression.

Miwa N., Nagano T., Jimbo N., Dokuni R., Kiriu T., Mimura C., Yasuda Y., Katsurada M., Yamamoto M., Tachihara M., Tanaka Y., Kobayashi K., Itoh T., Maniwa Y, & Nishimura Y. (2020). Caspase Recruitment Domain-Containing Protein 9 Expression is a Novel Prognostic Factor for Lung Adenocarcinoma. OncoTargets and therapy, 13, 9005-9013.

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Characterization of SCLC Cell Lines

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Nine SCLC cell lines (NCI-H1048; RRID: CVCL_1453, NCI-H1341; RRID: CVCL_1463, NCI-H841; RRID: CVCL_1595, DMS114; RRID: CVCL_1562, NCI-H211; RRID: CVCL_1529, NCI-H446 RRID: CVCL_1562, NCI-H889: RRID: CVCL_1598, NCI-H146; RRID: CVCL_1473, NCI-H524; RRID: CVCL_1568) were purchased from ATCC and maintained in cell culture. H211, H889, H1048, and H1341 cell lines are female and the rest are male. Cell lines were authenticated using short tandem repeat analysis, and were monthly tested for mycoplasma contamination. Cell media was RPMI-1640 supplemented with 10% FBS for all lines to maintain consistency. Cells were grown at 37˚C and 5% CO2. were used in subsequent experiments.

Takahashi N., Kim S., Schultz C.W., Rajapakse V.N., Zhang Y., Redon C.E., Fu H., Pongor L., Kumar S., Pommier Y., Aladjem M.I, & Thomas A. (2022). Replication Stress Defines Distinct Molecular Subtypes Across Cancers. Cancer Research Communications, 2(6), 503-517.

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