M205c stereomicroscope

Morphological examination of psyllid (Hemiptera: Psylloidea) specimens was conducted post non-destructive DNA extraction to confirm the presence of a Casuarinicola species, which was reported by the metabarcoding analysis within some samples (see below). Morphological identification was based on the taxonomical keys provided by Taylor et al. (2010) (link). Images of adult psyllids and of the exuviae of an immature were obtained stacking together from five to 30 images obtained using a Leica stereo microscope M205C with a DFC450 camera using the Leica Application Suite software v4.5.0. These images were used to generate Fig. 8.

The euthanasia of zebrafish larvae was carried out at the end of the experiments, with their immersion in a 4% tricaine solution diluted in 0.5 × E2 medium. Then, the absence of a heartbeat was confirmed using a Leica stereomicroscope (M205 C), after which a 10% bleach solution was added.

Specimens were collected through intensive hand searching and afterwards stored in 75% alcohol and examined using a Leica M205C stereomicroscope. Further details were studied under a Leica DM2500 compound microscope. All illustrations were made using a drawing tube and inked on ink jet plotter paper. Vulvae of females were cleared in lactic acid.
The following abbreviations are used in the text: ALE-anterior lateral eyes; PLE-posterior lateral eyes; IZCAS-Institute of Zoology, Chinese Academy of Sciences in Beijing.

Specimens were examined and measured with a Leica M205 C stereomicroscope. Details were studied with an Olympus BX51 compound microscope. Illustrations were made using a camera lucida attached to the Olympus BX51 microscope, and inked using an ink jet plotter. Male copulatory organs were examined and illustrated after they were dissected from the spiders. Type specimens were preserved in 75% ethanol solution. Photographs were taken with an Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on a Leica M205 C stereomicroscope. The images were assembled using Helicon Focus 3.10 image stacking software. All measurements are given in millimeters. Leg measurements are shown as: Total length (femur + patella + tibia + metatarsus + tarsus). Leg segments were measured on their dorsal side. Distribution map was generated with ArcView GIS 3.2. Terminology and taxonomic descriptions follow Huber (2000) . Type specimens are deposited in the Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology in Hanoi, Vietnam.
The following abbreviations are used in the description: ALE

= anterior lateral eye

, AME

= anterior median eye

, PME

= posterior median eye

, L/d

= length/diameter

.

Specimens were examined and measured with a Leica M205 C stereomicroscope. Left male pedipalps were photographed (unless otherwise indicated in figure legends). Epigynes were photographed before dissection. Vulvae were treated in a 10% warm solution of potassium hydroxide (KOH) to dissolve soft tissues before illustration. Images were captured with a Canon EOS 750D wide zoom digital camera (24.2 megapixels) mounted on the stereomicroscope mentioned above and assembled using Helicon Focus 3.10.3 image stacking software [27 ]. All measurements are given in millimeters (mm). Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). Leg segments were measured on their dorsal side. The specimens studied were preserved in 75% ethanol and deposited in the College of Life Science, Shenyang Normal University (SYNU) in Liaoning, China. Terminology and taxonomic descriptions follow Huber [21 ] and Yao et al. [18 (link),28 (link)]. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The LSID for this publication is: urn:lsid:zoobank.org:pub:4CAA98A6-2CF3-44C8-94A5-4B363E23B603. The distribution map was generated with ArcGIS 10.2 (ESRI Incorporated Company, Redlands, CA, USA).

The macrocouple current flowing between the gilding and the bronze is directly correlated by Faraday's Law, to the corrosion rate and is continuously monitored by means of a high precision Keithley 3706 multimeter. The same equipment has been used to measure the driving force for the galvanic corrosion and the electrical resistance of the artificial patina. The resolution of the multimeter is 0.01–100 μV in the 100 mV–300 V range, 0.1 μΩ–10 Ω in the 1 Ω–100 MΩ range and 1 pA–1 μA in the 10 μA–3 A range.
Stereomicroscopic observation of the patina and of the sensors surface has been performed using a Leica M205C stereomicroscope, equipped with a Leica DFC290 digital camera.
Infrared spectra of patinas were recorded in transmission mode, in the spectral range between 4,000 and 400 cm⁻¹ with 4 cm⁻¹ resolution, using a Thermo Electron Nicolet 6700 FTIR spectrometer.
A Philips PW1830X-Ray Diffractometer (XRD) with a PW3020 generator, in the Bragg-Brentano geometry and Thin Film, copper anticathode (Kα1 radiation;, was used for phase identification of patinas.
Environmental Scanning Electron Microscope (ESEM) was performed using a Zeiss EVO 50 EP instrument equipped with a LaB6 source and an Oxford INCA 200—Pentafet LZ4 X-ray spectrometer was used to observe patina morphology and to determine elemental compositions.