Explore vista

Manufactured by GE Healthcare

Sourced in United States

The EXplore VISTA is a PET/CT system designed for preclinical research applications. It combines high-performance PET imaging with CT imaging capabilities to provide anatomical and functional data. The system is optimized for imaging small animals such as rodents.

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Lab products found in correlation

Gamma counter, by PerkinElmer (2 mentions) Isoflurane, by Baxter (2 mentions) C rgdyk, by Peptide Institute (1 mentions) 125i echistatin, by PerkinElmer (1 mentions) Micropet r4 rodent model scanner, by Siemens (1 mentions) Inveon dpet, by Siemens (1 mentions)

22 protocols using explore vista

Small Animal FDG-PET Imaging Protocol

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PET scans were performed on a dedicated small animal PET/CT scanner (eXplore VISTA, GE Healthcare, WI). All animals were fasted for at least 8 h before the start of the study. Animals were anesthetized with 2% isoflurane at 1 L/min oxygen flow for 5–10 min. FDG (10.5–11.7 MBq) was injected by an intravenous bolus injection. Static emission scans at 45 min after the injection were acquired. During the FDG uptake period, animals were awake and then anesthetized 10 min before PET/CT scans to reduce anesthetic effects on microglia [27 (link)]. Emission scans were acquired for 20 min. The energy window for the scan was 400–700 keV and images were reconstructed by a three-dimensional ordered-subsets expectation maximum (OSEM) algorithm with attenuation, random and scatter correction. The final voxel size was 0.3875 × 0.3875 × 0.775 mm.

Choi H., Choi Y., Lee E.J., Kim H., Lee Y., Kwon S., Hwang D.W, & Lee D.S. (2021). Hippocampal glucose uptake as a surrogate of metabolic change of microglia in Alzheimer’s disease. Journal of Neuroinflammation, 18, 190.

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Tumor Targeting with 64Cu-Labeled Antibody

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PET imaging was performed four weeks after tumor inoculation with a method described previously.^{20 (link), 22 (link)} Briefly, each mouse was given 3.7 MBq of ⁶⁴Cu-DOTA-Pab-IR800 or ⁶⁴Cu-DOTA-IgG via the tail vein injection. The PET images were acquired at 4, 24, and 48 h post injection using a small animal PET/CT system (GE eXplore Vista) while the mice were under isoflurane-induced anesthesia. The regions of interest (ROIs) were calculated to percentage injected dose per gram tissue (%ID/g) based on the assumption of 1g/mL tissue density. Biodistribution study was performed in nude mice bearing NCI/ADR-RES tumors. Mice were given intravenously approximate 5.5 MBq of ⁶⁴Cu-DOTA-Pab-IR800 and ⁶⁴Cu-DOTA-IgG, and were sacrificed 48h post injection. Tissues and organs of interest were excised and weighed. Radioactivity was measured by a γ-counter (PerkinElmer) and the tracer uptake was calculated as %ID/g.

Wang M., Mao C., Wang H., Ling X., Wu Z., Li Z, & Ming X. (2017). Molecular Imaging of P-glycoprotein in Chemoresistant Tumors using a Dual-Modality PET/Fluorescence Probe. Molecular pharmaceutics, 14(10), 3391-3398.

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Imaging Neuroinflammation in Symptomatic EAE Mice

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Symptomatic EAE mice (n = 7) and CTR mice (n = 5) underwent PET/CT studies using high-resolution scanner (GE Healthcare eXplore Vista; PET resolution 1.8 mm FWHM, sensitivity 4.2% ACS, energy windows 250–700 keV; CT 200 μA, 35 kVp, resolution 200 μm) under inhalational anaesthesia (isoflurane 2%, oxygen 2 l/min). Images were acquired in dynamic mode (frame sequence 6 × 5 min) over 30 min starting 20 min after injection via tail vein of 5.55–7.00 MBq (specific radioactivity 200–800 GBq/μmol) of [¹⁸F]DPA-714. Images were processed using a 2D FORE/3D OSEM iterative algorithm (voxel size 0.3875 × 0.3875 × 0.775 mm) including random, scatter, dead time, and decay correction. Count rates were converted to standardized uptake values (SUV) tissue activity (MBq/cm³)/(injected dose (MBq)/body weight (g)). PET frames acquired between 20 and 50 min were summed and used for data analysis.

Coda A.R., Anzilotti S., Boscia F., Greco A., Panico M., Gargiulo S., Gramanzini M., Zannetti A., Albanese S., Pignataro G., Annunziato L., Salvatore M., Brunetti A., De Berardinis P., Quarantelli M., Palma G, & Pappatà S. (2020). In vivo imaging of CNS microglial activation/macrophage infiltration with combined [18F]DPA-714-PET and SPIO-MRI in a mouse model of relapsing remitting experimental autoimmune encephalomyelitis. European Journal of Nuclear Medicine and Molecular Imaging, 48(1), 40-52.

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PET/CT Imaging of BAT Metabolic Activity

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PET/CT scans and image analysis were performed using a small animal PET scanner (GE eXplore Vista) as previously reported [25 (link)–27 (link)]. [¹⁸F]BODIPY 1 (1.85 MBq each) was intravenously injected at 5–10 min after BAT stimulation. Static PET images (10 min) were then acquired at 1 h post injection (p.i.) of [¹⁸F]BODIPY 1. The images were reconstructed by the two-dimensional ordered subset expectation maximum (OSEM) algorithm. For each PET scan, volumes of interest (VOIs) were drawn over the muscle and BAT on decay-corrected whole-body coronal images. The average radioactivity concentration (accumulation) within the organ of interest was obtained from the mean pixel values, which was then converted to counts/ml/min using a conversion factor. Assuming a tissue density of 1 g/ml, the VOIs were converted to counts/g/min and then divided by the administered activity to obtain an image-derived %ID/g.

Wang H., Wang M., Chansaenpak K., Liu Y., Yuan H., Xie J., Yin H., Branca R.T., Li Z, & Wu Z. (2020). A Novel PET Probe for Brown Adipose Tissue Imaging in Rodents. Molecular imaging and biology, 22(3), 675-684.

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In-Vivo PET Imaging of Neuroreceptors

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PET scans were performed on a dedicated microPET/CT scanner (eXplore VISTA, GE Healthcare). All animals were anesthetized and maintained with 1% isoflurane at 1 L/min oxygen flow and placed on the prone position under a scanner. Rats received an intravenous bolus injection (0.2–0.5 mL/rat) of [¹¹C]ABP688 (7.0–17.1 MBq/100 g) and list-mode data were acquired for 60 min with the energy window 400–700 keV. These list mode data were framed into a dynamic sequence of 6×30 s, 7×60 s, and 5×600 s frames. The images were reconstructed by a 3-dimensional ordered-subsets expectation maximum (OSEM) algorithm with attenuation, random and scatter correction. The voxel size was 0.3875×0.3875×0.775 mm.

Choi H., Kim Y.K., Oh S.W., Im H.J., Hwang D.W., Kang H., Lee B., Lee Y.S., Jeong J.M., Kim E.E., Chung J.K, & Lee D.S. (2014). In Vivo Imaging of mGluR5 Changes during Epileptogenesis Using [11C]ABP688 PET in Pilocarpine-Induced Epilepsy Rat Model. PLoS ONE, 9(3), e92765.

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PET Imaging of Sonication-Induced Brain Metabolism

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After the sonication, the animal was removed from the sonication stage and was subjected to PET scanning (eXplore Vista; GE Healthcare, Waukesha, Wisconsin, USA). The reconstructed PET data consisted of 61 images of 175×175 in matrix size with 68×68 mm² in the field-of-view, resulting in voxel dimensions of 0.39×0.39×0.78 mm³, and the pixel-by-pixel average standardized uptake value (SUV) was calculated at 1 h after injection to detect the level of glucose metabolic activities in the brain 14 (link).
To detect the brain area showing an elevated SUV level because of sonication, the average SUV from the unsonicated area (which was 6×6 mm², marked as a black rectangle in Fig. 1a), which lies contralateral to the site of sonication, was measured to establish the baseline level of metabolic activity. Then, the pixels showing a greater SUV were located transversely and longitudinally along the sonication path (shown as arrows in Fig. 1a) to estimate the size of the stimulation profile.

Kim H., Lee S.D., Chiu A., Yoo S.S, & Park S. (2014). Estimation of the spatial profile of neuromodulation and the temporal latency in motor responses induced by focused ultrasound brain stimulation. Neuroreport, 25(7), 475-479.

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In Vivo PET Imaging of Mouse Brain

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Mice were anesthetized with 3% isoflurane (Baxter Medical) and medical
grade oxygen at a rate of 1 L/min. A CT scout scan was done first, followed by a
CT scan and a dynamic PET scan. Each mouse received the same dose per gram of
body weight, 1.75 Ci/g ^{18 (link)}Fluorodeoxyglucose (FDG) tracer solution by tail vein injection, followed by a
0.1 ml saline flush. Dynamic PET imaging for each mouse was immediately
performed for 1 hour (in vivo PET) using a small animal PET/CT scanner (eXplore
Vista; GE Healthcare). The spatial resolution of the PET scanner was 1.6 mm at
the center of the field-of-view. The data were acquired in 3D mode at the energy
window of 250–700 keV, which yields 4% count sensitivity. For each
timepoint, 5 or 6 3-D volumes, spanning cortical and subcortical regions, were
selected in the center of the brain of the animal, with volumes of .2
cm³ each, and used for quantification using the eXplore Vista
software. Averages of these regions were used as the standard uptake value (SUV)
for the animal.

Zullo J.M., Drake D., Aron L., O’Hern P., Dhamne S.C., Davidsohn N., Mao C.A., Klein W.H., Rotenberg A., Bennett D.A., Church G.M., Colaiácovo M.P, & Yankner B.A. (2019). Regulation of Lifespan by Neural Excitation and REST. Nature, 574(7778), 359-364.

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In Vivo Evaluation of 64Cu-DOTA-NT-Cy5.5

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Animal procedures were performed according to a protocol approved by the University of North Carolina Institutional Animal Care and Use Committee. In brief, each mouse bearing HT-29 xenograft was injected with approximately 3.7 MBq of ⁶⁴Cu-DOTA-NT-Cy5.5 via the tail vein (n = 3/group). The same amount of activity was injected into the blocking group with unlabeled NT (100 μg) through the tail vein. The imaging data were achieved at 1 and 4 h p.i. using a small animal PET scanner (GE eXplore Vista), with the mice under anesthesia using isoflurane (3% for induction and 2% for maintenance). The regions of interest (ROIs) were converted to counts per gram per minute based on the assumption of 1 g/mL tissue density. Dividing counts per gram per minute by injected dose gave the image ROI-derived percentage injected dose per gram tissue (%ID/g) values.
Biodistributions were performed in nude mice bearing HT-29 tumor. Animals were sacrificed under inhalation anesthesia at 4 h postinjection of 3.7 MBq of ⁶⁴Cu-DOTA-NT-Cy5.5. Tissues and organs of interest were excised and weighed. Radioactivity in each excised specimen was measured using a γ-counter (PerkinElmer). The mean uptake (%ID/g) was calculated for each group of animals.

Deng H., Wang H., Wang M., Li Z, & Wu Z. (2015). Synthesis and Evaluation of 64Cu-DOTA-NT-Cy5.5 as a Dual-Modality PET/Fluorescence Probe to Image Neurotensin Receptor-Positive Tumor. Molecular pharmaceutics, 12(8), 3054-3061.

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Quantifying mGluR5 Availability in Neuropathic Pain

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mGluR5 availability levels were measured using PET imaging data acquired in our previous study [24 (link)]. Briefly, the SNL group and sham group rats underwent PET scans with a mGluR5-specific radiotracer, [¹¹C] ABP688, using a microPET scanner (eXplore VISTA, GE Healthcare, Waukesha, Wisconsin, USA) at 16–25 days after surgery. The list-mode data for 60 min were acquired and reconstructed into a single static image and multiple dynamic frames. mGluR5 availability was measured by calculating the non-displaceable binding potential (BP_ND) of [¹¹C] ABP688 using a simplified reference tissue model with the cerebellum as a reference region. All images were co-registered, normalized, resampled, smoothed, and analyzed using SPM8 and SPM12 software. A voxel-by-voxel two-sample t-test was performed and the statistical map was overlaid on the magnetic resonance imaging (MRI) template [30 (link)] for visualization. To compare mGluR5 availability in the S1DZ in this study, a 0.5 mm radius spherical region-of-interest (ROI) was defined using the MarsBaR toolbox [31 (link)], at the location of S1DZ with the following coordinates: ML ±4.6 mm, AP −0.2 mm and DV −3.4 mm from the bregma. Proportionally scaled values were extracted for each subject.

Chung G., Yun Y.C., Kim C.Y., Kim S.K, & Kim S.J. (2022). Metabotropic Glutamate Receptor 5 in the Dysgranular Zone of Primary Somatosensory Cortex Mediates Neuropathic Pain in Rats. Biomedicines, 10(7), 1633.

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PET and CT Imaging in Mice

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A dedicated small animal PET scanner (eXplore VISTA; GE Healthcare) and small animal CT scanner (X-SPECT/CT; Gamma Medica) were used. In all experiments, the PET session was conducted for two mice simultaneously with the same batch of [¹¹C]A836339. One mouse from a pair represented a control group (NTG or no-treatment group) whereas the other mouse represented an experimental group (transgenic or treatment group). Mice were induced and anesthetized with isoflurane. Dynamic PET scans were acquired for 30 min (20 sec x 3, 30 sec x 2, 1 min x 2, 2 min x 3, 5 min x 4) immediately after an intravenous bolus injection of [¹¹C]A836339 (0.11–0.14 mCi, specific radioactivity 8,600 ± 2,100 mCi/μmol). A CT scan was acquired shortly after the PET scan as a reference for localization of brain regional radiotracer uptake through PET-CT image fusion performed off-line. In all PET experiments, a 250–700 keV energy window was used, and the data were reconstructed using an iterative 2D ordered-subject expectation-maximization method, using a trans-axial pixel size of 0.4 mm and axial slice thickness of 0.8 mm [46 (link)]. No attenuation and scatter corrections were applied, as they have relatively small impact on mouse brain imaging.

Savonenko A.V., Melnikova T., Wang Y., Ravert H., Gao Y., Koppel J., Lee D., Pletnikova O., Cho E., Sayyida N., Hiatt A., Troncoso J., Davies P., Dannals R.F., Pomper M.G, & Horti A.G. (2015). Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation. PLoS ONE, 10(6), e0129618.

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