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Qev column

Manufactured by PerkinElmer
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The QEV column is a laboratory equipment designed for sample preparation and purification. It utilizes a proprietary resin to efficiently extract and concentrate analytes from complex matrices. The column's core function is to facilitate the isolation and concentration of target compounds prior to further analysis or processing.

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Lab products found in correlation

Alexa fluor 488 nhs, by Thermo Fisher Scientific (2 mentions) Nanosight ns300, by Malvern Panalytical (2 mentions) Micro bca assay, by Thermo Fisher Scientific (2 mentions) Enspire, by PerkinElmer (2 mentions)

2 protocols using qev column

1

Fluorescent Labeling of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
To conduct the EV labelling reaction, 2 × 1011 purified LAC-CM-EVs were diluted into a total volume of 500 μL PBS. The EV solution was then mixed with 5 μL of 10 mg mL−1 NHS-Alexa Fluor 488 (Molecular Probes, Thermo Fisher Scientific) to ensure a homogeneous dye distribution. The labelling reaction was allowed to proceed for 12 h at 4 °C. To purify LAC-CM-EVs, qEV columns (Izon Sciences) were used, with elution profiles being characterised for the (1) unlabelled EVs, (2) labelled LAC-CM-EVs and (3) free labelling buffer. Each 500 μL eluting from the qEV column was collected and assayed for total fluorescence intensity (Enspire, PerkinElmer), protein content was assayed by Micro-BCA assay (Thermo Fisher Scientific) and particle concentration was assessed using NTA as described previously (Nanosight NS300, Malvern Instruments).
Zwi-Dantsis L., Winter C.W., Kauscher U., Ferrini A., Wang B., Whittaker T.E., Hood S.R., Terracciano C.M, & Stevens M.M. (2020). Highly purified extracellular vesicles from human cardiomyocytes demonstrate preferential uptake by human endothelial cells. Nanoscale, 12(38), 19844-19854.
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2

Fluorescent Labeling of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
To conduct the EV labelling reaction, 2 × 1011 purified LAC-CM-EVs were diluted into a total volume of 500 μL PBS. The EV solution was then mixed with 5 μL of 10 mg mL−1 NHS-Alexa Fluor 488 (Molecular Probes, Thermo Fisher Scientific) to ensure a homogeneous dye distribution. The labelling reaction was allowed to proceed for 12 h at 4 °C. To purify LAC-CM-EVs, qEV columns (Izon Sciences) were used, with elution profiles being characterised for the (1) unlabelled EVs, (2) labelled LAC-CM-EVs and (3) free labelling buffer. Each 500 μL eluting from the qEV column was collected and assayed for total fluorescence intensity (Enspire, PerkinElmer), protein content was assayed by Micro-BCA assay (Thermo Fisher Scientific) and particle concentration was assessed using NTA as described previously (Nanosight NS300, Malvern Instruments).
Zwi-Dantsis L., Winter C.W., Kauscher U., Ferrini A., Wang B., Whittaker T.E., Hood S.R., Terracciano C.M, & Stevens M.M. (2020). Highly purified extracellular vesicles from human cardiomyocytes demonstrate preferential uptake by human endothelial cells. Nanoscale, 12(38), 19844-19854.
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