Bx61 fluoview confocal microscope

Manufactured by Olympus

Sourced in United States

The Olympus BX61 Fluoview confocal microscope is a versatile imaging system designed for high-resolution fluorescence microscopy. It features a laser-scanning confocal architecture that enables optical sectioning and the acquisition of three-dimensional images. The microscope is equipped with multiple laser excitation wavelengths and sophisticated imaging capabilities to support a wide range of fluorescence-based applications.

Automatically generated - may contain errors

Lab products found in correlation

3 well slides, by Ibidi (1 mentions) Fit phalloidin based f actin red fluorescence, by Abcam (1 mentions) Alexa 488 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Cy3 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Fluoview microscope BX61 BX61 confocal microscope FluoView confocal microscope Fluoview BX61 BX61 microscope Confocal microscope

2 protocols using bx61 fluoview confocal microscope

Visualizing Cytoskeletal and Cell Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

The FIT-phalloidin-based F-actin red fluorescence (Abcam, Cambridge, UK) staining was done on HUVEC cells alone or HUVEC co-cultured with D283 and D425 cells on 3-well slides (ibidi, Fitchburg, WI, USA). The cells were grown for 24 h, fixed and stained according to the vendor’s instructions. Calcein AM green fluorescence (Invitrogen, Carlsbad, CA, USA) was used to stain D283 and D425 cells in the co-culture experiment. The images were captured with an Olympus BX61 Fluoview confocal microscope (Olympus, Center Valley, PA, USA) at 40× magnification. The immunostaining detection of B7-H3 protein on the MB tissue sections and cells was done following a standard protocol [21 (link)].

Purvis I.J., Avilala J., Guda M.R., Venkataraman S., Vibhakar R., Tsung A.J., Velpula K.K, & Asuthkar S. (2019). Role of MYC-miR-29-B7-H3 in Medulloblastoma Growth and Angiogenesis. Journal of Clinical Medicine, 8(8), 1158.

+ Open protocol

+ Expand

Immunofluorescence Imaging of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were grown to 50–75% confluency on 8 chamber slides (Nalge Lab-Tek II, ThermoFisher, Rochester, NY), washed in PBS, and fixed for 2 hrs. in Streck tissue fixative (Streck Laboratories, Omaha, NE). Cells were permeabilized with 0.5% Triton X-100 in 2% fetal bovine serum, 2% horse serum, phosphate-buffered saline, pH 7.4. Antigens were visualized indirectly using the secondary antibodies Alexa 488-conjugated goat anti-mouse IgG (Molecular Probes, Eugene, OR) or Cy3-conjugated goat anti-rabbit IgG (Jackson Immunoresearch Labs, West Grove, PA). Nuclei were stained with DAPI. Immunofluorescent micrographs were viewed employing an Olympus BX61 Fluoview confocal microscope with the 60X objective. Images were acquired using Olympus Fluoview software (version 1.7a). Isotype-matched IgG control antibodies yielded negative staining results in all cases.

Jemmerson R., Staskus K., Higgins L., Conklin K, & Kelekar A. (2021). Intracellular leucine-rich alpha-2-glycoprotein-1 competes with Apaf-1 for binding cytochrome c in protecting MCF-7 breast cancer cells from apoptosis. Apoptosis : an international journal on programmed cell death, 26(1-2), 71-82.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!