Tacrolimus

Manufactured by Bio-Techne

Tacrolimus is a laboratory reagent used in research applications. It functions as a calcineurin inhibitor, which plays a role in the regulation of immune responses. Tacrolimus is commonly used in the study of cellular signaling pathways and immune system processes.

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Lab products found in correlation

Fk506, by Bio-Techne (2 mentions) Rs127445, by Bio-Techne (1 mentions) Cyclosporine a csa, by Bio-Techne (1 mentions) L glutamic acid, by Bio-Techne (1 mentions) D ap5, by Bio-Techne (1 mentions)

3 protocols using tacrolimus

Modulating Inflammation in Mouse Models

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All experiments were approved by the local Ethical Committee on Animal Research, Malmö/Lund (permission nos: M140-09, M189-12). Female BALB/c (LPS model) and C57Bl/6 (SEA model) mice obtained from Taconic, Denmark were acclimatized for a minimum of one week prior to use in any experiments. The mice were pre-treated with drug compounds (AM1030, RS127445, tacrolimus, cyclosporine A (CsA; Tocris Bioscience, Cat. no: 1101)) or vehicle (as described in Figure legends) before intraperitoneal injection of LPS (0.5 mg/kg in normal saline) or SEA (0.5 mg/kg in normal saline). The animals were sacrificed 90 (LPS) or 120 (SEA) min after LPS/SEA injection and blood was collected for plasma analyses. Samples were stored at -20 °C until analysis.

Palmqvist N., Siller M., Klint C, & Sjödin A. (2016). A human and animal model-based approach to investigating the anti-inflammatory profile and potential of the 5-HT2B receptor antagonist AM1030. Journal of Inflammation (London, England), 13, 20.

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Glutamate-Induced MEA Activity in SCN

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TTX, D-AP5, DNQX, L-glutamic acid and FK506 (Tacrolimus) were purchased from Tocris Bioscience, Sigma-Aldrich or Stratech. Drugs were dissolved in distilled water or dimethyl sulfoxide to make stock aliquots at 1000–10,000× final concentrations and stored at −20 °C until required. Stock aliquots were diluted with oxygenated aCSF to final concentration immediately before use.
To assess the effect of glutamate on MEA activity of acute SCN slices, we bath applied different concentrations of glutamate (0, 10, 30 and 100 µM) with recording commencing 5 min after starting perfusion and continuing for 2 min. The same slice was used with successively increasing concentrations of glutamate.

Lalic T., Steponenaite A., Wei L., Vasudevan S.R., Mathie A., Peirson S.N., Lall G.S, & Cader M.Z. (2020). TRESK is a key regulator of nocturnal suprachiasmatic nucleus dynamics and light adaptive responses. Nature Communications, 11, 4614.

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Tacrolimus Modulates Tfh Cells in Fracture Healing

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The calcineurin inhibitor tacrolimus (FK506, Tocris Bioscience) was used to identify the contributions of Tfh cells to the post fracture immune response. tacrolimus specifically suppresses both lymph node Tfh cells and circulating Tfh cells, but not their regulatory counterparts or other CD4 T cell subsets (Wallin et al., 2018 (link)). In the current study fracture mice were treated with FK506 (5 mg/kg) or vehicle via subcutaneous injection 3 times a week (once every other day from Monday to Friday) for 3 weeks, followed by cast removal, nociceptive testing and tissue harvesting. This dosage was derived from prior literature demonstrating FK506 efficacy in reducing autoimmune dermatitis in transgenic mice with mixed connective tissue disease (Loser et al., 2006 (link)).

Li W.W., Yang Y., Shi X.Y., Guo T.Z., Guang Q., Kingery W.S., Herzenberg L.A, & Clark J.D. (2020). Germinal Center Formation, Immunoglobulin Production and Hindlimb Nociceptive Sensitization after Tibia Fracture. Brain, behavior, and immunity, 88, 725-734.

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