A modular HPLC system (Shimadzu Prominence, Shimadzu Corporation, Kyoto, Kyoto Prefecture, Japan) equipped with a degasser (DGU-20A5), quaternary pump (LC-20AT), an autosampler (SIL-20 A HT), a system controller (CBM-20 A), a column oven (CTO-20 A), a photodiode array detector (SPD-M20AV) was used during all analysis. Chromatographic data management was performed using LC Solutions (Version. 5.2).
Spd m20av
Manufactured by Shimadzu
Sourced in Japan
The SPD-M20AV is a photodiode array (PDA) detector for high-performance liquid chromatography (HPLC) systems. It is designed to provide sensitive and reliable detection of a wide range of compounds. The SPD-M20AV collects and processes spectral data across a broad wavelength range, enabling comprehensive analysis of sample components.
Automatically generated - may contain errors
Lab products found in correlation
Sil 20a, by Shimadzu (5 mentions)
Microtof 2, by Bruker (4 mentions)
Lc 20 ad apparatus, by Shimadzu (4 mentions)
Esi qtof mass spectrometer, by Bruker (4 mentions)
Xcalibur 2, by Thermo Fisher Scientific (2 mentions)
Lc 20at, by Shimadzu (1 mentions)
Cbm 20a, by Shimadzu (1 mentions)
Sil 20a ht, by Shimadzu (1 mentions)
Cto 20a, by Shimadzu (1 mentions)
Dgu 20a5, by Shimadzu (1 mentions)
Dataanalysis 4, by Bruker (1 mentions)
Lc 20ar, by Shimadzu (1 mentions)
7 protocols using spd m20av
1
HPLC Analysis Using Shimadzu System
2
Identification of Bioactive Phytochemicals
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The U. alba DCM sub-extract (UaD),
being the most biologically active, was chemically profiled for the
identification of its putative secondary metabolites using LC-HR-ESIMS-QToF
performed on a Shimadzu LC-20 AD apparatus equipped with an autosampler
(SIL-20A, Shimadzu), diode array detector (SPD-M20AV, Shimadzu), and
coupled with a microToF II (Bruker Daltonics) ESI-QToF mass spectrometer.
HPLC column Chromolith Performance RP-18e (2.0 × 100 mm i.d.)
was used for the analysis. The eluents were acetonitrile and water
with 0.1% acetic acid. After injecting 5 μL of the UaD sub-extract,
flow elution was set at 0.2 mL/min. The effluents were monitored at
350 nm. The mass spectra were recorded in the mass range m/z 50 to 2000. The Bruker DataAnalysis 4.3 software
(Bruker, Germany) was used for data acquisition and analysis. Individual
components were identified by comparison of their m/z values in the total ion count profile with those
compounds described in literature or by matching their MS/MS spectra
with those reported in a public repository of mass spectral data called
MassBank.40 (link)
being the most biologically active, was chemically profiled for the
identification of its putative secondary metabolites using LC-HR-ESIMS-QToF
performed on a Shimadzu LC-20 AD apparatus equipped with an autosampler
(SIL-20A, Shimadzu), diode array detector (SPD-M20AV, Shimadzu), and
coupled with a microToF II (Bruker Daltonics) ESI-QToF mass spectrometer.
HPLC column Chromolith Performance RP-18e (2.0 × 100 mm i.d.)
was used for the analysis. The eluents were acetonitrile and water
with 0.1% acetic acid. After injecting 5 μL of the UaD sub-extract,
flow elution was set at 0.2 mL/min. The effluents were monitored at
350 nm. The mass spectra were recorded in the mass range m/z 50 to 2000. The Bruker DataAnalysis 4.3 software
(Bruker, Germany) was used for data acquisition and analysis. Individual
components were identified by comparison of their m/z values in the total ion count profile with those
compounds described in literature or by matching their MS/MS spectra
with those reported in a public repository of mass spectral data called
MassBank.40 (link)
3
Chemical Profiling of UaB Metabolites
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UaB was chemically profiled to identify its
putative secondary metabolites using untargeted LC-HRMS analysis.
This was performed on a Shimadzu LC-20 AD apparatus equipped with
an autosampler (SIL-20A, Shimadzu), a diode array detector (SPD-M20AV,
Shimadzu), and coupled with a microToF II (Bruker Daltonics) ESI-QToF
mass spectrometer. High-performance liquid chromatography column Chromolith
Performance RP-18e (2.0 × 100 mm2 i.d.) was used for
the analysis. The eluents were acetonitrile and water with 0.1% acetic
acid. After injecting 5 μL of the UaB fraction, flow elution
was set at 0.2 mL/min. The peaks were monitored at 350 nm. The mass
spectra were recorded in the mass range m/z 50–2000. Bruker DataAnalysis 4.3 software (Bruker,
Germany) was used for data acquisition and analysis. Individual components
were identified by comparison of their m/z values in the total ion count profile with those compounds
described in the literature or by matching their MS/MS spectra with
those reported in a public repository of mass spectral data called
MassBank.
putative secondary metabolites using untargeted LC-HRMS analysis.
This was performed on a Shimadzu LC-20 AD apparatus equipped with
an autosampler (SIL-20A, Shimadzu), a diode array detector (SPD-M20AV,
Shimadzu), and coupled with a microToF II (Bruker Daltonics) ESI-QToF
mass spectrometer. High-performance liquid chromatography column Chromolith
Performance RP-18e (2.0 × 100 mm2 i.d.) was used for
the analysis. The eluents were acetonitrile and water with 0.1% acetic
acid. After injecting 5 μL of the UaB fraction, flow elution
was set at 0.2 mL/min. The peaks were monitored at 350 nm. The mass
spectra were recorded in the mass range m/z 50–2000. Bruker DataAnalysis 4.3 software (Bruker,
Germany) was used for data acquisition and analysis. Individual components
were identified by comparison of their m/z values in the total ion count profile with those compounds
described in the literature or by matching their MS/MS spectra with
those reported in a public repository of mass spectral data called
MassBank.
4
NIR-Triggered PTX Release from HCuSNPs
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In the study of long-term release of PTX from HCuSNPs-MS-PTX, a solution of HCuSNPs-MS-PTX (200 µL, 0.46 mg/mL, containing 2.285 µg/mL of PTX) containing 0.5% Tween 80 was aspirated and then placed into an EP vial in a 37 °C water bath. At various times three vials were centrifuged at 13,523 × g for 10 min, and the free PTX was quantified by a high performance liquid chromatography (HPLC) system (LC-20AR, Shimadzu, Japan) with an SPD-M20AV photodiode array detector (Shimadzu, Japan).
The NIR-light-triggered release of PTX was investigated with two samples, which were HCuSNPs-MS-PTX precipitate (10 mg) and a PTX-MS precipitate suspended with PBS (0.01 mol/L, 200 μL, pH 7.4) containing Tween 80 (0.5%, w/v). The two samples were exposed to 915-nm NIR laser light at a power density of 0.5 W/cm2 for 3 min. The above procedure was repeated three times and the solution was centrifuged at 3214 × g for 5 min to obtain the supernatant fraction for free PTX determination. The HCuSNPs-MS-PTX solution that did not receive laser irradiation was used as control.
The NIR-light-triggered release of PTX was investigated with two samples, which were HCuSNPs-MS-PTX precipitate (10 mg) and a PTX-MS precipitate suspended with PBS (0.01 mol/L, 200 μL, pH 7.4) containing Tween 80 (0.5%, w/v). The two samples were exposed to 915-nm NIR laser light at a power density of 0.5 W/cm2 for 3 min. The above procedure was repeated three times and the solution was centrifuged at 3214 × g for 5 min to obtain the supernatant fraction for free PTX determination. The HCuSNPs-MS-PTX solution that did not receive laser irradiation was used as control.
5
High-Res LC-MS and Low-Res LC-MS Analyses
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High resolution analyses by LC-MS were performed on a Shimadzu LC-20 AD apparatus equipped with an autosampler (SIL-20A, Shimadzu), diode array detector (SPD-M20AV, Shimadzu) and coupled with a micrOTOFII (Bruker Daltonics) ESI-qTOF mass spectrometer. The LC conditions were the same applied at High Performance Liquid Chromatography coupled with diode array (HPLC-DAD) Analysis . The column eluent was split at a ratio of 7:3, where the larger flow went to the DAD detector and the lower one went to the mass spectrometer.
Low resolution applied a similar Shimadzu LC-20 AD apparatus coupled with an ESI-ion trap mass spectrometer (amaZon, Bruker Daltonics). Again, the LC conditions were the same as described onHigh Performance Liquid Chromatography coupled with diode array (HPLC-DAD) Analysis . The column eluent was split at a ratio of 7:3, where the larger flow went to the DAD detector and the lower one went to the mass spectrometer.
Low resolution applied a similar Shimadzu LC-20 AD apparatus coupled with an ESI-ion trap mass spectrometer (amaZon, Bruker Daltonics). Again, the LC conditions were the same as described on
6
Chemical Profiling of U. alba DCM Sub-extract
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The U. alba DCM sub-extract (UaD), being the most biologically active, was chemically profiled for the identification of its putative secondary metabolites using LC-HR-ESIMS-QToF performed on a Shimadzu LC-20 AD apparatus equipped with an autosampler (SIL-20A, Shimadzu), diode array detector (SPD-M20AV, Shimadzu) and coupled with a micrOTOFII (Bruker Daltonics) ESI-qTOF mass spectrometer. HPLC column Chromolith Performance RP-18e (2.0 x 100 mm i.d.) was used for the analysis. The eluents were acetonitrile and water with 0.1% acetic acid. After injecting 5 μL of the UaD sub-extract, flow elution was set at 2 mL/min. The effluents were monitored at 350 nm. The mass spectra were recorded in the mass range m/z 50 to 2000. The Xcalibur 2.2 software (Thermo Fisher Scientific, USA) was used for data acquisition and analysis.
7
Chemical Profiling of U. alba DCM Sub-extract
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The U. alba DCM sub-extract (UaD), being the most biologically active, was chemically profiled for the identification of its putative secondary metabolites using LC-HR-ESIMS-QToF performed on a Shimadzu LC-20 AD apparatus equipped with an autosampler (SIL-20A, Shimadzu), diode array detector (SPD-M20AV, Shimadzu) and coupled with a micrOTOFII (Bruker Daltonics) ESI-qTOF mass spectrometer. HPLC column Chromolith Performance RP-18e (2.0 x 100 mm i.d.) was used for the analysis. The eluents were acetonitrile and water with 0.1% acetic acid. After injecting 5 μL of the UaD sub-extract, flow elution was set at 2 mL/min. The effluents were monitored at 350 nm. The mass spectra were recorded in the mass range m/z 50 to 2000. The Xcalibur 2.2 software (Thermo Fisher Scientific, USA) was used for data acquisition and analysis.
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