Luminex multi analyte profiling system xmap

Manufactured by DiaSorin

Sourced in United States

The Luminex Multi-Analyte Profiling System (xMAP) is a versatile laboratory equipment for multiplex analysis. The system utilizes magnetic microbeads and flow cytometry technology to simultaneously detect and quantify multiple analytes in a single sample.

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Lab products found in correlation

Labtype sso hla dpa1 dpb1 kit, by Thermo Fisher Scientific (2 mentions) Qiaamp dna blood kit, by Qiagen (1 mentions) Vacutainer cell preparation tube, by BD (1 mentions) Genomiphi v2 dna amplification kit, by GE Healthcare (1 mentions)

6 protocols using luminex multi analyte profiling system xmap

HLA-DRB1 Genotyping and ACPA Assessment

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The methods for determining the HLA-DRB1 alleles have previously been reported.28 (link) In brief, the four-digit HLA-DRB1 genotyping was performed by using the LABType HD Class II DRB1 sequence specific oligonucleotide assay (One Lambda, California, USA) with the Luminex Multi-Analyte Profiling System (xMAP, Luminex Corporation, Texas, USA). The HLA-DRB1 SE alleles were defined as the presence of DRB1*01:01, DRB1*01:02, DRB1*01:07, DRB1*04:01, DRB1*04:04, DRB1*04:05, DRB1*04:08, DRB1*04:10, DRB1*10:01 and DRB1*10:03. Individuals with one or two SE alleles are categorised as SE-positive. ACPA status was assessed by using an anti-cyclic citrullinated peptide (anti-CCP) second-generation ELISA kits (Immunoscan RA, Malmö, Sweden). Samples with results >25 AU/mL were defined as positive.28 (link)
31 (link)

Too C.L., Muhamad N.A., Ilar A., Padyukov L., Alfredsson L., Klareskog L., Murad S, & Bengtsson C. (2015). Occupational exposure to textile dust increases the risk of rheumatoid arthritis: results from a Malaysian population-based case–control study. Annals of the Rheumatic Diseases, 75(6), 997-1002.

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Isolation and HLA Typing of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were separated from heparinized blood samples by density centrifugation using BD Vacutainer Cell Preparation Tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Genomic DNA was isolated from PBMCs with QIAamp DNA Blood Kits (Qiagen, Germantown, MD, USA) for HLA typing using the Luminex Multi-Analyte profiling system (xMAP; Luminex, Austin, TX, USA) [17 (link)].

Nagai H., Suzukawa M., Sakakibara Y., Ohta K., Reche P.A., Suzuki K, & Hoshino Y. (2014). Immunological Responses and Epitope Mapping by Tuberculosis-Associated Antigens within the RD1 Region in Japanese Patients. Journal of Immunology Research, 2014, 764028.

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High-Resolution HLA Genotyping in Asia

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High resolution (4-digit) genotyping of HLA-DPA1 and -DPB1 alleles was performed for HBV patients, resolved individuals, and healthy controls in Japan, Korea, Hong Kong, and Thailand. LABType SSO HLA DPA1/DPB1 kit (One Lambda, CA) and a Luminex Multi-Analyte Profiling system (xMAP; Luminex, Austin, TX) were used for genotyping, in according with the manufacturer's protocol. Because of the small quantity of genomic DNA in some Korean samples, we performed whole genome amplification for a total of 486 samples using GenomiPhi v2 DNA Amplification kit (GE Healthcare Life Sciences, UK), in accordance with the manufacturer's instruction.
A total of 2,895 samples were successfully genotyped and characteristics of these samples are summarized in Table S1.

Nishida N., Sawai H., Kashiwase K., Minami M., Sugiyama M., Seto W.K., Yuen M.F., Posuwan N., Poovorawan Y., Ahn S.H., Han K.H., Matsuura K., Tanaka Y., Kurosaki M., Asahina Y., Izumi N., Kang J.H., Hige S., Ide T., Yamamoto K., Sakaida I., Murawaki Y., Itoh Y., Tamori A., Orito E., Hiasa Y., Honda M., Kaneko S., Mita E., Suzuki K., Hino K., Tanaka E., Mochida S., Watanabe M., Eguchi Y., Masaki N., Murata K., Korenaga M., Mawatari Y., Ohashi J., Kawashima M., Tokunaga K, & Mizokami M. (2014). New Susceptibility and Resistance HLA-DP Alleles to HBV-Related Diseases Identified by a Trans-Ethnic Association Study in Asia. PLoS ONE, 9(2), e86449.

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HLA Genotyping in SLE Patients

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All patients with SLE were genotyped for classical HLA class I (ie, HLA-A, HLA-B and HLA-C) and HLA class II alleles (ie, HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1) using PCR sequence-specific oligonucleotides (PCR-SSO) probe hybridisation method (LIFECODES HLA SSO Typing Kit, Gene Probe, USA) with Luminex Multi-Analyte Profiling System (xMAP, Luminex, Texas, USA), according to manufacturer’s instructions. The assignment of the specific HLA alleles was accomplished using the Quick Type for Life Match 2.6.1 software for Gen-Probe analysis.

Selvaraja M., Too C.L., Tan L.K., Koay B.T., Abdullah M., Shah A.M., Arip M, & Amin-Nordin S. (2022). Human leucocyte antigens profiling in Malay female patients with systemic lupus erythematosus: are we the same or different?. Lupus Science & Medicine, 9(1), e000554.

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HLA Genotyping Using PCR-SSO

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The experimental classical HLA genotyping for HLA-A, HLA-B, HLA-C, HLA-DRB1, and HLA-DQB1 genes was performed previously and described elsewhere [9 (link), 25 (link)]. In brief, the HLA genotyping was performed for all DNA samples using the polymerase chain reaction and sequence-specific oligonucleotide probe hybridization (PCR-SSO) method (LABType® HLA test kits, One Lambda Inc., CA, USA) on the Luminex Multi-Analyte Profiling System (xMAP, Luminex Corporation, TX, USA). The HLA typing assignment was accomplished using the HLA Fusion software (version 1.3.0) provided by the manufacturer (One Lambda Inc., CA, USA).

Tan L.K., Too C.L., Diaz-Gallo L.M., Wahinuddin S., Lau I.S., Heselynn H., Nor-Shuhaila S., Gun S.C., Eashwary M., Mohd-Shahrir M.S., Ainon M.M., Azmillah R., Muhaini O., Shahnaz M., Alfredsson L., Klareskog L, & Padyukov L. (2021). The spectrum of association in HLA region with rheumatoid arthritis in a diverse Asian population: evidence from the MyEIRA case-control study. Arthritis Research & Therapy, 23, 46.

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HLA Typing and Imputation Concordance

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HLA typing was conducted using LABType SSO HLA DPA1/DPB1 kit (One Lambda, CA) and a Luminex Multi-Analyte Profiling system (xMAP; Luminex, Austin, TX) according to the manufacturer’s protocol. The genotyping results were compared to the imputation data and concordance rates were calculated.

Ashouri S., Khor S.S., Hitomi Y., Sawai H., Nishida N., Sugiyama M., Kawai Y., Posuwan N., Tangkijvanich P., Komolmit P., Tsuiji M., Shotelersuk V., Poovorawan Y., Mizokami M, & Tokunaga K. (2022). Genome-Wide Association Study for Chronic Hepatitis B Infection in the Thai Population. Frontiers in Genetics, 13, 887121.

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