Nova nanosem 450 system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Nova NanoSEM 450 is a high-resolution scanning electron microscope system designed for advanced imaging and analysis applications. It features a field emission electron source, high-performance electron optics, and a range of detection capabilities to enable detailed characterization of nanoscale structures and materials.

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Lab products found in correlation

Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions) Asap 2020, by Micromeritics (1 mentions) Syringe pump, by Harvard Apparatus (1 mentions) Multianodephotomultiplier, by Nikon (1 mentions) Dimension icon system, by Bruker (1 mentions) A1r mp system, by Nikon (1 mentions) Eclipse ti, by Nikon (1 mentions) Avatar 360 nicolet instrument, by Thermo Fisher Scientific (1 mentions) Tecnai g2 f30 system, by Thermo Fisher Scientific (1 mentions) Chi660e electrochemical workstation, by Chenhua (1 mentions) 33 dual syringe pump, by Harvard Apparatus (1 mentions)

Close spelling variants of this product

Nova NanoSEM 450 Nova NanoSEM NanoSEM 450 Nova 450

8 protocols using nova nanosem 450 system

Scanning Electron Microscopy Protocol

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Scanning
electron microscopy (SEM) was performed with an FEI Nova NanoSEM 450
system, equipped with an energy-dispersive X-ray (EDX) detector and
operating at acceleration voltages of 5–10 kV (Scheme 1).

Moschetto S., Bolognesi M., Prescimone F., Brucale M., Mezzi A., Ortolani L., Caporali M., Pingue P., Serrano-Ruiz M., Pisignano D., Peruzzini M., Persano L, & Toffanin S. (2021). Large-Area Oxidized Phosphorene Nanoflakes Obtained by Electrospray for Energy-Harvesting Applications. ACS Applied Nano Materials, 4(4), 3476-3485.

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Comprehensive Material Characterization by Advanced Microscopy and Spectroscopy

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SEM images and EDS were obtained from a Nova Nano SEM 450 system (FEI, Hillsborough, OR, USA) equipped with the TEAMEDS energy dispersive spectrometer. The studied samples were casted on the surface of glassy carbon electrodes customized for electron microscopy (Chuxi Instruments Co., Shanghai, China) to form a thin film layer. The dehydrated specimens were sprayed with platinum before examination. FTIR spectra were measured by a Nicolet 6700 FTIR spectrometer (Thermo Fisher, Waltham, MA, USA). The samples were prepared by the KBr disc or film technique. The Zeta potential of different samples were determined by dynamic light scattering (DLS) (Zem4228, Malvern Instruments, Malvern, UK). The specific surface areas of different samples were calculated by the Brunauer–Emmett–Teller (BET) method (ASAP-2020, Micromeritics, Atlanta, GA, USA). All electrochemical measurements were carried out on a CHI660E electrochemical workstation (Chenhua Instruments Co., Shanghai, China). The three-electrode system was composed of a working electrode (GCE, 3.0 mm in diameter), a platinum wire counter electrode and a silver chloride reference electrode.

Li M., Wu J., Su H., Tu Y., Shang Y., He Y, & Liu H. (2019). Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms for Hydrogen Peroxide Sensing. Sensors (Basel, Switzerland), 19(3), 640.

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Electrospinning of BBQ/PMMA Fibers

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BBQ/PMMA solutions at 1% wt/wt are
prepared by CHCl₃ (PMMA content = 3.75 wt % compared to
solvent). The concentration is chosen in the range 0.1–2% following
preliminary tests performed to optimize the emission. The solution
is mixed by mechanical stirring for 12 h at room temperature prior
to electrospinning, which is performed by a 1 mL syringe and a 21
gauge stainless needle. Randomly oriented fibers are spun by an applied
voltage of 15 kV (EL60R0.6-22, Glassman High Voltage) and a needle-collector
distance of 20 cm. The electrospinning injection flow rate is kept
constant at 1 mL h^–1 using a syringe pump (Harvard
Apparatus). Aligned fibers are obtained using a collector having a
diameter of 8 cm and rotating at 4000 rpm, placed 10 cm from the needle.
Reference films are spin-cast from the same solution used for electrospinning.
Sample inspection is performed by a Nova NanoSEM 450 system (FEI).

Morello G., Manco R., Moffa M., Persano L., Camposeo A, & Pisignano D. (2015). Multifunctional Polymer Nanofibers: UV Emission, Optical Gain, Anisotropic Wetting, and High Hydrophobicity for Next Flexible Excitation Sources. ACS Applied Materials & Interfaces, 7(39), 21907-21912.

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Nanoscale Pt Deposition via FIB-SEM

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The Bi₂Te₃ flake with uniform surface quality was selected and milled by FIB with 30 KeV Ga⁺ ions inside a FEI Nova Nano SEM450 system (Hillsboro, OR, USA). The angle between the ion beam and the electron beam is 52°. After adding the gas-phase Pt, metallic nanostructures were deposited by scanning a 15 KeV electron beam on the selected area. The surface morphology was determined by scanning electron microscope (SEM), and the elementary composition was confirmed by energy-dispersive X-ray spectroscopy (EDX) in the same chamber.

Yan Q., Li X, & Liang B. (2020). Plasmonic Emission of Bullseye Nanoemitters on Bi2Te3 Nanoflakes. Materials, 13(7), 1531.

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Multi-Modal Characterization of Nanopatterned Surfaces

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Confocal micrographs are acquired by an inverted microscope Eclipse Ti equipped with a confocal A1R-MP system (Nikon), using an Argon ion laser (excitation wavelength, λ = 488 nm). The sample emission is collected by a 60× (oil immersion NA = 1.40, Nikon) objectives and the fluorescence signal is detected by a spectral detection unit equipped with a multi-anode photomultiplier (Nikon).
The AFM characterization of the nanopatterned surfaces is carried out by “peak force” imaging mode in air using a Bruker Dimension Icon system equipped with a Nanoscope V controller. The used silicon tip (nominal radius of curvature of 2 nm) is mounted on silicon nitride cantilever with 0.4 N/m nominal spring constant. SEM is performed with a Nova NanoSEM 450 system (FEI), using an acceleration voltage around 8 kV and an aperture size of 30 mm.

Portone A., Ganzer L., Branchi F., Ramos R., Caldas M.J., Pisignano D., Molinari E., Cerullo G., Persano L., Prezzi D, & Virgili T. (2019). Tailoring optical properties and stimulated emission in nanostructured polythiophene. Scientific Reports, 9, 7370.

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Characterization of Cu-TCPP Catalyst

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The X-ray diffraction (XRD, X’Pert PRO diffractometer, Cu Ka, Panalytical Company, Almelo, The Netherlands) was employed to characterize the crystal structure. A Fourier transform infrared (FT-IR) was analyzed on an Avatar 360 Nicolet instrument (Thermo Fisher Scientific, Shanghai, China). Scanning electron microscopic images and transmission electron microscopic images were measured using a Nova Nano SEM 450 system and the Tecnai G2 F30 system, respectively (FEI Company, Eindhoven, The Netherlands). A micromeritics ASAP 2020 analyzer (Norcross, GA, USA) was used to test Nitrogen adsorption–desorption isotherms.
A CHI 660E electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd., Shanghai, China) was used for electrochemical measurements. The Cu-TCPP modified glassy carbon electrode (GCE, diameter: 3 mm), saturated calomel electrode (SCE), and platinum wire were used as the working electrode, reference electrode, and counter electrode, respectively.

Ji L., Wang Q., Peng L., Li X., Zhu X, & Hu P. (2022). Cu-TCPP Nanosheets-Sensitized Electrode for Simultaneous Determination of Hydroquinone and Catechol. Materials, 15(13), 4625.

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Electrospinning of MEH-PPV Nanofibers

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Fibers
are produced by
electrospinning a solution (70–200 μM polymer) of poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene]
(MEH-PPV) (molecular weight 380 000 g/mol, American Dye Source
Inc.). Sprayed films of microbeads and microfibers are obtained at
concentrations >200 μM. The polymer is dissolved in a 1:4
(weight:weight)
mixture of dimethyl sulfoxide (DMSO) and tetrahydrofuran (THF). The
electrospinning system consists of a microprocessor dual drive syringe
pump (33 Dual Syringe Pump, Harvard Apparatus Inc.), feeding the polymer
solution through the metallic needle at constant rate (10 μL/min).
A 11 kV bias is applied between the needle and a metallic collector
(needle-collector distance 6 cm), made of two Al stripes positioned
at a mutual distance of 2 cm. The MEH-PPV nanofibers are collected
on a 1 × 1 cm² quartz substrate for optical investigation.
Arrays of uniaxially aligned nanofibers are also produced by using
a rotating collector (4000 rpm, corresponding to a linear velocity
of 30 m/s at the disk edge) for emission polarization measurements,
featuring similar morphology and optical properties as samples deposited
on the Al stripes. The fiber morphology is investigated by scanning
electron microscopy (SEM) using a Nova NanoSEM 450 system (FEI), with
an acceleration voltage of 5–10 kV.

Camposeo A., Greenfeld I., Tantussi F., Moffa M., Fuso F., Allegrini M., Zussman E, & Pisignano D. (2014). Conformational Evolution of Elongated Polymer Solutions Tailors the Polarization of Light-Emission from Organic Nanofibers. Macromolecules, 47(14), 4704-4710.

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Knee Joint Matrix Ultrastructural Analysis

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After fixation of the knee joints, the samples were placed in 70% ethanol. The bone tunnel was carefully cut open along the sagittal axis with the matrix intact. The matrix was divided into three segments: intra-articular region, mid-tunnel, and tunnel exit region, followed by dehydration in increasing concentration of ethanol (80% to 100%). Samples were then dried with a critical point dryer. Samples were mounted onto stubs and the base coated with silver paint to enhance the conductivity of the sample. Samples were imaged on the FEI Nova NanoSEM 450 system.

Mengsteab P.Y., Conroy P., Badon M., Otsuka T., Kan H.M., Vella A.T., Nair L.S, & Laurencin C.T. (2020). Evaluation of a bioengineered ACL matrix’s osteointegration with BMP-2 supplementation. PLoS ONE, 15(1), e0227181.

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