Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12) and antibiotic–antimycotic (penicillin G and streptomycin) solution were purchased from Gibco, Invitrogen, USA. Fetal bovine serum (FBS) was procured from Himedia, India. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was supplied by BioVision, USA. The antibodies used in western blotting against β-actin, p53, Bax, Bcl-2, cleaved caspase-3, AKT, p-AKT473, mTOR, p-mTOR2448 were purchased from Cell Signaling Technology (Danvers, MA). Other chemicals were obtained from Sigma-Aldrich, USA and were of analytical grade.
P mtor2448
Manufactured by Cell Signaling Technology
P-mTOR2448 is an antibody that recognizes the phosphorylated form of mammalian target of rapamycin (mTOR) at the serine 2448 residue. mTOR is a serine/threonine protein kinase that plays a central role in the regulation of cell growth, proliferation, and survival.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin g, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by HiMedia (2 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (2 mentions)
Pakt473, by Cell Signaling Technology (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Hoechst 33342, by HiMedia (2 mentions)
Rhodamine 123 rh 123, by HiMedia (2 mentions)
Ethidium bromide etbr, by HiMedia (2 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Propidium iodide ribonuclease a rnase a 2 7 dichlorodihydrofluorescein diacetate dcfh da acridine orange, by HiMedia (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc propidium iodide apoptosis detection kit, by Abcam (1 mentions)
2 protocols using p mtor2448
1
DMEM/F-12 Media Cytotoxicity Assay
2
Apoptosis Induction Assay in Cells
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Dulbecco's Modi ed Eagle Medium Nutrient Mixture F-12 (DMEM/F-12) and antibiotic-antimycotic (penicillin G and streptomycin) solution were purchased from Gibco, Invitrogen, USA. Fetal bovine serum (FBS) was procured from Himedia, India. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydro uorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. Annexin V-uorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was supplied by BioVision, USA. The antibodies used in western blotting against β-actin, p53, Bax, Bcl-2, cleaved caspase-3, Akt, p-Akt 473 , mTOR, p-mTOR 2448 were purchased from Cell Signaling Technology (Danvers, MA). Other chemicals were obtained from Sigma-Aldrich, USA and were of analytical grade.
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