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Eq bead standards

Manufactured by Standard BioTools
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EQ bead standards are a set of beads designed for instrument calibration and performance verification. They are used to ensure the accurate measurement of particle size and concentration in various analytical instruments.

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Lab products found in correlation

Cell id cisplatin 195pt, by Standard BioTools (3 mentions) Human trustain fcx, by BioLegend (2 mentions) Helios cytof 3, by Standard BioTools (2 mentions) Human trustain fcx blocking solution, by BioLegend (1 mentions)

3 protocols using eq bead standards

1

Comprehensive PBMC Immunophenotyping by CyTOF

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5x106 peripheral blood mononuclear cells (PBMCs) were stained with Cell-ID Cisplatin-195Pt (Fluidigm, South San Francisco, CA) to discriminate dead cells. PBMCs were then FcR blocked with Human TruStain FcX (Biolegend, San Diego, CA), stained with the antibody mix as shown in Table S2, fixed with 1.6% paraformaldehyde, and stained with Cell-ID intercalator-Ir. Stained PBMCs were analyzed in a Helios CyTOF 3.0 (Fluidigm) following manufacturer’s instructions at the Cancer and Immunology Core at Vanderbilt University. The data was exported as a Flow Cytometry Standard file (FCS) and normalized using EQ bead standards (Fluidigm) following manufacturer’s protocol.
Garcia-Solis B., Van Den Rym A., Pérez-Caraballo J.J., Al–Ayoubi A., Alazami A.M., Lorenzo L., Cubillos-Zapata C., López-Collazo E., Pérez-Martínez A., Allende L.M., Markle J., Fernández-Arquero M., Sánchez-Ramón S., Recio M.J., Casanova J.L., Mohammed R., Martinez-Barricarte R, & Pérez de Diego R. (2021). Clinical and Immunological Features of Human BCL10 Deficiency. Frontiers in Immunology, 12, 786572.
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2

Profiling Dead PBMC Subsets

Cited 1 time
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In total, 5 × 106 PBMCs were stained with Cell-ID Cisplatin-195Pt (Fluidigm [now Standard BioTools], South San Francisco, Calif) to detect dead cells. The PBMCs were then subjected to FcR blockade with Human TruStain FcX blocking solution (BioLegend, San Diego, Calif), stained with the antibody mixture, as previously described16 (link) (see Table E1 in this article’s Online Repository at www.jacionline.org), fixed with 1.6% paraformaldehyde, and stained with Cell-ID intercalator-Ir. The stained PBMCs were then analyzed in a Helios CyTOF 3.0 cytometer (Fluidigm) at the Vanderbilt University Cancer and Immunology Core. The data were exported as a flow cytometry standard (FCS) file and normalized against EQ bead standards (Fluidigm) according to the manufacturer’s protocol.
García-Solís B., Van Den Rym A., Martinez-Martínez L., Franco T., Pérez-Caraballo J.J., Markle J., Cubillos-Zapata C., Marín A.V., Recio M.J., Regueiro J.R., Navarro-Zapata A., Mestre-Durín C., Ferreras C., Cotázar C.M., Mena R., de la Calle-Fabregat C., López-Lera A., Arquero M.F., Pérez-Martínez A., López-Collazo E., Sánchez-Ramón S., Casanova J.L., Martínez-Barricarte R., de la Calle-Martín O, & de Diego R.P. (2023). Inherited human ezrin deficiency impairs adaptive immunity. The Journal of allergy and clinical immunology, 152(4), 997-1009.e11.
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3

CyTOF Phenotyping of PBMC

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Staining and data acquisition: 5x10 6 peripheral blood mononuclear cells (PBMCs) were stained with Cell-ID Cisplatin-195 Pt (Fluidigm, South San Francisco, CA) to discriminate dead cells. PBMCs were then FcR blocked with Human TruStain FcX (Biolegend, San Diego, CA), stained with the antibody mix as shown in table S1, xed with 1.6% paraformaldehyde, and stained with Cell-ID intercalator-Ir. Stained PBMCs were analyzed in a Helios CyTOF 3.0 (Fluidigm) following manufacturer's instructions at the Cancer and Immunology Core at Vanderbilt University. The data was exported as a Flow Cytometry Standard le (FCS) and normalized using EQ bead standards (Fluidigm) following manufacturer's protocol.
Amador-Borrero B., Rym A.M.V.D., Pérez-Caraballo J.J., –Ayoubi A.A., Lorenzo L., Cubillos‐Zapata C., López‐Collazo E., Markle J., Fernández‐Arquero M., Sánchez‐Ramón S., Recio M.J., Casanova J., Mohammed R., Martínez‐Barricarte R., & Diego R.P.d. (2021). Clinical and Immunological Features of Human BCL10 Deficiency.
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