The miR-873a-5p mimic, negative control mimic (NC mimic) and agomir were purchased from RiboBio (China). They were dissolved and diluted according to the instructions provided by the manufacturer. The microglia were transfected with 100 nM aliquots of either the miR-873a-5p mimic or NC mimic using RiboFECT™ CP (RiboBio, China) as per the manufacturer’s protocol. After dissolving and mixing the miR-873a-5p agomir, it was allowed to stand for 15 min at room temperature and was then used for lateral ventricle injection. A Hamilton syringe (Gaoge, China) was inserted at 0.5 mm posterior and 1.0 mm lateral to the bregma and 3.0 mm ventral to the skull under the guidance of a stereotaxic instrument (RWD Life Science). A single dose of miR-873a-5p agomir (5 nM) was infused into the lateral ventricle 20 min after CCI.
Mir 873a 5p mimic
Manufactured by RiboBio
Sourced in China
MiR-873a-5p mimic is a synthetic RNA molecule that mimics the mature sequence of the microRNA miR-873a-5p. MicroRNAs are small, non-coding RNA molecules that play a role in regulating gene expression.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mir 873a 5p mimic
1
Mimic and Agomir Regulation of miR-873a-5p
2
Modulating miR-873a-5p in Traumatic Brain Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The miR-873a-5p mimic, negative control mimic (NC mimic), and agomir were purchased from RiboBio (China). They were dissolved and diluted according to the instructions provided by the manufacturer. The microglia were transfected with 100 nM aliquots of either the miR-873a-5p mimic or NC mimic using RiboFECT™ CP (RiboBio, China) per the manufacturer's protocol. After dissolving and mixing the miR-873a-5p agomir, it was allowed to stand for 15 minutes at room temperature and was then used for lateral ventricle injection. A Hamilton syringe (Gaoge, China) was inserted at 0.5 mm posterior and 1.0 mm lateral to the bregma and 3.0 mm ventral to the skull under the guidance of a stereotaxic instrument (RWD Life Science). Single dose of miR-873a-5p agomir (5 nM) were infused into the lateral ventricle 20 min after CCI.
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